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Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation.

Carlson PE, Carr KA, Janes BK, Anderson EC, Hanna PC - PLoS ONE (2009)

Bottom Line: Two genes encoding putative internalin proteins were chosen for further study.This attenuation was amplified in a double mutant strain.These data define the transcriptional changes induced during growth in low iron conditions and illustrate the potential of this dataset in the identification of putative virulence determinants for future study.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America.

ABSTRACT
Lack of available iron is one of many environmental challenges that a bacterium encounters during infection and adaptation to iron starvation is important for the pathogen to efficiently replicate within the host. Here we define the transcriptional response of B. anthracis Sterne (34F(2)) to iron depleted conditions. Genome-wide transcript analysis showed that B. anthracis undergoes considerable changes in gene expression during growth in iron-depleted media, including the regulation of known and candidate virulence factors. Two genes encoding putative internalin proteins were chosen for further study. Deletion of either gene (GBAA0552 or GBAA1340) resulted in attenuation in a murine model of infection. This attenuation was amplified in a double mutant strain. These data define the transcriptional changes induced during growth in low iron conditions and illustrate the potential of this dataset in the identification of putative virulence determinants for future study.

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Related in: MedlinePlus

Categorical analysis of genes induced during iron starvation.Pie charts represent the COG classification for the genes uniquely regulated in the conditions listed. Each pie represents 100% of the genes exhibiting significant gene expression changes following growth in iron depleted media. COG categories were identified for each differentially regulated gene based on genomic annotation (GenBank accession, NC_007530). To simplify the analysis, the COG categories were grouped into general categories as follows: Categories A, J, K, L, and RNA genes were merged into “Transcription/translation/DNA replication/RNA”; C, E, F, G, H, I, P, and Q were combined into “Transport and metabolism”; COGs N, T, and U were combined into “Cellular functions/trafficking/secretion/signal transduction”; and categories R and S were grouped with uncategorized genes and classified as “Unknown.” Other COG categories listed are reported as they were originally defined.
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pone-0006988-g004: Categorical analysis of genes induced during iron starvation.Pie charts represent the COG classification for the genes uniquely regulated in the conditions listed. Each pie represents 100% of the genes exhibiting significant gene expression changes following growth in iron depleted media. COG categories were identified for each differentially regulated gene based on genomic annotation (GenBank accession, NC_007530). To simplify the analysis, the COG categories were grouped into general categories as follows: Categories A, J, K, L, and RNA genes were merged into “Transcription/translation/DNA replication/RNA”; C, E, F, G, H, I, P, and Q were combined into “Transport and metabolism”; COGs N, T, and U were combined into “Cellular functions/trafficking/secretion/signal transduction”; and categories R and S were grouped with uncategorized genes and classified as “Unknown.” Other COG categories listed are reported as they were originally defined.

Mentions: In order to more thoroughly examine these results, the uniquely regulated genes were grouped based on the annotated COGs (Clusters of Orthologous Groups). Related COGs were grouped together into the categories shown in Figure 4. The pie charts show the categorical designation of the unique genes that are induced or repressed after four hours in IDM (Figure 4). Each chart represents 100% of the COG designations for the genes that were induced or repressed as discussed above. Each slice within a given chart represents the percentage of genes identified from a given category. By examining these general relationships between genes regulated by iron starvation, many interesting observations can be made.


Transcriptional profiling of Bacillus anthracis Sterne (34F2) during iron starvation.

Carlson PE, Carr KA, Janes BK, Anderson EC, Hanna PC - PLoS ONE (2009)

Categorical analysis of genes induced during iron starvation.Pie charts represent the COG classification for the genes uniquely regulated in the conditions listed. Each pie represents 100% of the genes exhibiting significant gene expression changes following growth in iron depleted media. COG categories were identified for each differentially regulated gene based on genomic annotation (GenBank accession, NC_007530). To simplify the analysis, the COG categories were grouped into general categories as follows: Categories A, J, K, L, and RNA genes were merged into “Transcription/translation/DNA replication/RNA”; C, E, F, G, H, I, P, and Q were combined into “Transport and metabolism”; COGs N, T, and U were combined into “Cellular functions/trafficking/secretion/signal transduction”; and categories R and S were grouped with uncategorized genes and classified as “Unknown.” Other COG categories listed are reported as they were originally defined.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2742718&req=5

pone-0006988-g004: Categorical analysis of genes induced during iron starvation.Pie charts represent the COG classification for the genes uniquely regulated in the conditions listed. Each pie represents 100% of the genes exhibiting significant gene expression changes following growth in iron depleted media. COG categories were identified for each differentially regulated gene based on genomic annotation (GenBank accession, NC_007530). To simplify the analysis, the COG categories were grouped into general categories as follows: Categories A, J, K, L, and RNA genes were merged into “Transcription/translation/DNA replication/RNA”; C, E, F, G, H, I, P, and Q were combined into “Transport and metabolism”; COGs N, T, and U were combined into “Cellular functions/trafficking/secretion/signal transduction”; and categories R and S were grouped with uncategorized genes and classified as “Unknown.” Other COG categories listed are reported as they were originally defined.
Mentions: In order to more thoroughly examine these results, the uniquely regulated genes were grouped based on the annotated COGs (Clusters of Orthologous Groups). Related COGs were grouped together into the categories shown in Figure 4. The pie charts show the categorical designation of the unique genes that are induced or repressed after four hours in IDM (Figure 4). Each chart represents 100% of the COG designations for the genes that were induced or repressed as discussed above. Each slice within a given chart represents the percentage of genes identified from a given category. By examining these general relationships between genes regulated by iron starvation, many interesting observations can be made.

Bottom Line: Two genes encoding putative internalin proteins were chosen for further study.This attenuation was amplified in a double mutant strain.These data define the transcriptional changes induced during growth in low iron conditions and illustrate the potential of this dataset in the identification of putative virulence determinants for future study.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America.

ABSTRACT
Lack of available iron is one of many environmental challenges that a bacterium encounters during infection and adaptation to iron starvation is important for the pathogen to efficiently replicate within the host. Here we define the transcriptional response of B. anthracis Sterne (34F(2)) to iron depleted conditions. Genome-wide transcript analysis showed that B. anthracis undergoes considerable changes in gene expression during growth in iron-depleted media, including the regulation of known and candidate virulence factors. Two genes encoding putative internalin proteins were chosen for further study. Deletion of either gene (GBAA0552 or GBAA1340) resulted in attenuation in a murine model of infection. This attenuation was amplified in a double mutant strain. These data define the transcriptional changes induced during growth in low iron conditions and illustrate the potential of this dataset in the identification of putative virulence determinants for future study.

Show MeSH
Related in: MedlinePlus