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Changes in frequency of spontaneous oscillations in procerebrum correlate to behavioural choice in terrestrial snails.

Samarova E, Balaban P - Front Cell Neurosci (2009)

Bottom Line: In vivo recordings in freely behaving naïve snails showed a significant decrease of spontaneous PC oscillations frequency during a stage of tentacle withdrawal to odor presentation.No significant difference in responses to 5% and 20% cineole was noted.Obtained results suggest that frequency of the PC lobe spontaneous oscillations correlate to the choice of behavior in snails: withdrawal (decrease in frequency) or approach (increase in frequency) to the source of odor.

View Article: PubMed Central - PubMed

Affiliation: Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Science Moscow, Russia.

ABSTRACT
The aim of our study was to understand functional significance of spontaneous oscillations of local field potential in the olfactory brain lobe of terrestrial snail, the procerebrum (PC). We compared changes in frequency of oscillations in semi-intact preparations from snails trained to percept the same conditioned odor as positive (associated with food reinforcement) or negative (associated with noxious reinforcement). In vivo recordings in freely behaving naïve snails showed a significant decrease of spontaneous PC oscillations frequency during a stage of tentacle withdrawal to odor presentation. In in vitro preparations from naïve snails, a similar decrease in frequency of the PC oscillations to odor presentation was observed. Changes in frequency of the oscillations to cineole presentations in the "aversive" group of snails (demonstrating withdrawal) were much more pronounced than in naïve snails. No significant difference in responses to 5% and 20% cineole was noted. Changes in the spontaneous oscillations frequency in the snails trained to respond with positive reaction (approach) to cineole depended on the concentration of the applied odor, and these responses were qualitatively similar to responses of other groups during the first 10 s of responses to odor, but significantly different (increase in PC oscillations frequency) from the responses of the aversively trained and naïve snails in the interval 11-30 s, which corresponds to the end of the tentacle withdrawal and timing of decision making (approach or escape) in the free behaving snails. Obtained results suggest that frequency of the PC lobe spontaneous oscillations correlate to the choice of behavior in snails: withdrawal (decrease in frequency) or approach (increase in frequency) to the source of odor.

No MeSH data available.


(A) The olfactometer used for odor application; (B) Scheme of the experimental arrangement for training snails to odor cues and testing in the open-field experiments (locations of odor source are marked by crosses). The snail is shown in a start position; (C) Scheme of the semi-intact preparation of the CNS with the attached intact olfactory nerve, additional chamber for recording activity of buccal nerves, and the nose positioned on a small platform.
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Figure 1: (A) The olfactometer used for odor application; (B) Scheme of the experimental arrangement for training snails to odor cues and testing in the open-field experiments (locations of odor source are marked by crosses). The snail is shown in a start position; (C) Scheme of the semi-intact preparation of the CNS with the attached intact olfactory nerve, additional chamber for recording activity of buccal nerves, and the nose positioned on a small platform.

Mentions: The experiments were performed both in vivo in adult snails (Helix lucorum L.) and in vitro using semi-intact preparations with intact olfactory nerves and sensory pads on the tip of the tentacles (Figure 1C). The snails were kept in an active state, maintained in a humid environment and were fed cabbage. All efforts were made to minimize pain and quantity of animals. The Ethics Commission of Institute of Higher Nervous Activity, Russian Academy of Sciences, approved the experimental protocol.


Changes in frequency of spontaneous oscillations in procerebrum correlate to behavioural choice in terrestrial snails.

Samarova E, Balaban P - Front Cell Neurosci (2009)

(A) The olfactometer used for odor application; (B) Scheme of the experimental arrangement for training snails to odor cues and testing in the open-field experiments (locations of odor source are marked by crosses). The snail is shown in a start position; (C) Scheme of the semi-intact preparation of the CNS with the attached intact olfactory nerve, additional chamber for recording activity of buccal nerves, and the nose positioned on a small platform.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2742667&req=5

Figure 1: (A) The olfactometer used for odor application; (B) Scheme of the experimental arrangement for training snails to odor cues and testing in the open-field experiments (locations of odor source are marked by crosses). The snail is shown in a start position; (C) Scheme of the semi-intact preparation of the CNS with the attached intact olfactory nerve, additional chamber for recording activity of buccal nerves, and the nose positioned on a small platform.
Mentions: The experiments were performed both in vivo in adult snails (Helix lucorum L.) and in vitro using semi-intact preparations with intact olfactory nerves and sensory pads on the tip of the tentacles (Figure 1C). The snails were kept in an active state, maintained in a humid environment and were fed cabbage. All efforts were made to minimize pain and quantity of animals. The Ethics Commission of Institute of Higher Nervous Activity, Russian Academy of Sciences, approved the experimental protocol.

Bottom Line: In vivo recordings in freely behaving naïve snails showed a significant decrease of spontaneous PC oscillations frequency during a stage of tentacle withdrawal to odor presentation.No significant difference in responses to 5% and 20% cineole was noted.Obtained results suggest that frequency of the PC lobe spontaneous oscillations correlate to the choice of behavior in snails: withdrawal (decrease in frequency) or approach (increase in frequency) to the source of odor.

View Article: PubMed Central - PubMed

Affiliation: Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Science Moscow, Russia.

ABSTRACT
The aim of our study was to understand functional significance of spontaneous oscillations of local field potential in the olfactory brain lobe of terrestrial snail, the procerebrum (PC). We compared changes in frequency of oscillations in semi-intact preparations from snails trained to percept the same conditioned odor as positive (associated with food reinforcement) or negative (associated with noxious reinforcement). In vivo recordings in freely behaving naïve snails showed a significant decrease of spontaneous PC oscillations frequency during a stage of tentacle withdrawal to odor presentation. In in vitro preparations from naïve snails, a similar decrease in frequency of the PC oscillations to odor presentation was observed. Changes in frequency of the oscillations to cineole presentations in the "aversive" group of snails (demonstrating withdrawal) were much more pronounced than in naïve snails. No significant difference in responses to 5% and 20% cineole was noted. Changes in the spontaneous oscillations frequency in the snails trained to respond with positive reaction (approach) to cineole depended on the concentration of the applied odor, and these responses were qualitatively similar to responses of other groups during the first 10 s of responses to odor, but significantly different (increase in PC oscillations frequency) from the responses of the aversively trained and naïve snails in the interval 11-30 s, which corresponds to the end of the tentacle withdrawal and timing of decision making (approach or escape) in the free behaving snails. Obtained results suggest that frequency of the PC lobe spontaneous oscillations correlate to the choice of behavior in snails: withdrawal (decrease in frequency) or approach (increase in frequency) to the source of odor.

No MeSH data available.