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Concentration polarization of high-density lipoprotein and its relation with shear stress in an in vitro model.

Meng W, Yu F, Chen H, Zhang J, Zhang E, Dian K, Shi Y - J. Biomed. Biotechnol. (2009)

Bottom Line: HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus.With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased.The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, West China Medical Center, Sichuan University, Chengdu, China.

ABSTRACT
The purpose of this study was to determine the concentration polarization of high-density lipoprotein (HDL) at the surface of the carotid artery under conditions of steady flow and to establish its relationship with shear stress using an in vitro vascular simulation model of carotid bifurcation. Shear stress, HDL concentration at the surface, and the ratio of HDL concentration at the surface to concentration in bulk flow were measured at different locations within the model under high-speed (1.451 m/s) and low-speed (0.559 m/s) flow. HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus. With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased. The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).

Show MeSH
(a) Pictoral illustration of the carotid bifurcation vascular model. CCA: common carotid artery, ICA: internal carotid artery, ECA: external carotid artery, ICAS: internal carotid artery sinus. (b) The distribution of the shear stress in the carotid bifurcation vascular model. Color changes show the degree of shear stress. Blue represents the lowest shear stress while red is the highest. 1 and 2: control locations of CCA and ICA; 3 and 4: anterior and posterior edges of the low shear stress region, 5: core region of the low shear stress area.
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fig2: (a) Pictoral illustration of the carotid bifurcation vascular model. CCA: common carotid artery, ICA: internal carotid artery, ECA: external carotid artery, ICAS: internal carotid artery sinus. (b) The distribution of the shear stress in the carotid bifurcation vascular model. Color changes show the degree of shear stress. Blue represents the lowest shear stress while red is the highest. 1 and 2: control locations of CCA and ICA; 3 and 4: anterior and posterior edges of the low shear stress region, 5: core region of the low shear stress area.

Mentions: As shown in Figure 2(a), the model included the common carotid artery (CCA), external carotid artery (ECA), internal carotid artery (ICA), and internal carotid artery sinus (ICAS). The low shear stress core region and its margins were marked on the model. The low shear stress core region was marked as point 5, and the anterior and posterior margins were marked as points 3 and 4 (Figure 2(b)). The measuring points of the inner diameter of the CCA and the inner diameter of the ICA were used as control points and marked as points 1 and 2 (Figure 2(b)). In addition, the final model is provided in the schematic diagram in Figure 3(a), and the schematic diagram of the experimental system was shown in Figure 3(b).


Concentration polarization of high-density lipoprotein and its relation with shear stress in an in vitro model.

Meng W, Yu F, Chen H, Zhang J, Zhang E, Dian K, Shi Y - J. Biomed. Biotechnol. (2009)

(a) Pictoral illustration of the carotid bifurcation vascular model. CCA: common carotid artery, ICA: internal carotid artery, ECA: external carotid artery, ICAS: internal carotid artery sinus. (b) The distribution of the shear stress in the carotid bifurcation vascular model. Color changes show the degree of shear stress. Blue represents the lowest shear stress while red is the highest. 1 and 2: control locations of CCA and ICA; 3 and 4: anterior and posterior edges of the low shear stress region, 5: core region of the low shear stress area.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2742649&req=5

fig2: (a) Pictoral illustration of the carotid bifurcation vascular model. CCA: common carotid artery, ICA: internal carotid artery, ECA: external carotid artery, ICAS: internal carotid artery sinus. (b) The distribution of the shear stress in the carotid bifurcation vascular model. Color changes show the degree of shear stress. Blue represents the lowest shear stress while red is the highest. 1 and 2: control locations of CCA and ICA; 3 and 4: anterior and posterior edges of the low shear stress region, 5: core region of the low shear stress area.
Mentions: As shown in Figure 2(a), the model included the common carotid artery (CCA), external carotid artery (ECA), internal carotid artery (ICA), and internal carotid artery sinus (ICAS). The low shear stress core region and its margins were marked on the model. The low shear stress core region was marked as point 5, and the anterior and posterior margins were marked as points 3 and 4 (Figure 2(b)). The measuring points of the inner diameter of the CCA and the inner diameter of the ICA were used as control points and marked as points 1 and 2 (Figure 2(b)). In addition, the final model is provided in the schematic diagram in Figure 3(a), and the schematic diagram of the experimental system was shown in Figure 3(b).

Bottom Line: HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus.With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased.The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, West China Medical Center, Sichuan University, Chengdu, China.

ABSTRACT
The purpose of this study was to determine the concentration polarization of high-density lipoprotein (HDL) at the surface of the carotid artery under conditions of steady flow and to establish its relationship with shear stress using an in vitro vascular simulation model of carotid bifurcation. Shear stress, HDL concentration at the surface, and the ratio of HDL concentration at the surface to concentration in bulk flow were measured at different locations within the model under high-speed (1.451 m/s) and low-speed (0.559 m/s) flow. HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus. With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased. The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).

Show MeSH