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Concentration polarization of high-density lipoprotein and its relation with shear stress in an in vitro model.

Meng W, Yu F, Chen H, Zhang J, Zhang E, Dian K, Shi Y - J. Biomed. Biotechnol. (2009)

Bottom Line: HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus.With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased.The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, West China Medical Center, Sichuan University, Chengdu, China.

ABSTRACT
The purpose of this study was to determine the concentration polarization of high-density lipoprotein (HDL) at the surface of the carotid artery under conditions of steady flow and to establish its relationship with shear stress using an in vitro vascular simulation model of carotid bifurcation. Shear stress, HDL concentration at the surface, and the ratio of HDL concentration at the surface to concentration in bulk flow were measured at different locations within the model under high-speed (1.451 m/s) and low-speed (0.559 m/s) flow. HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus. With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased. The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).

Show MeSH
Observation of cell growth and cell arrangement with a converted microscope after implanting the inner surface of the carotid bifurcation model (magnification  ×  100).
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig1: Observation of cell growth and cell arrangement with a converted microscope after implanting the inner surface of the carotid bifurcation model (magnification × 100).

Mentions: Semipermeable poly tetra fluor ethylene (PTFE) was employed to create the carotid bifurcation model. The inner diameter of each region of a human carotid artery was measured by Doppler ultrasound and computerized tomography angiography. Because a previous model at 1:1 resulted in uneven endothelialization and other technical difficulties, these measurements were amplified at a rate of 1:1.5 (Table 1) experimentally to create a bifurcation model of the carotid artery that was subsequently coated with endothelial cells on the inner wall of the model to induce the endothelialization of the inner wall of the model as shown in Figure 1 [26].


Concentration polarization of high-density lipoprotein and its relation with shear stress in an in vitro model.

Meng W, Yu F, Chen H, Zhang J, Zhang E, Dian K, Shi Y - J. Biomed. Biotechnol. (2009)

Observation of cell growth and cell arrangement with a converted microscope after implanting the inner surface of the carotid bifurcation model (magnification  ×  100).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2742649&req=5

fig1: Observation of cell growth and cell arrangement with a converted microscope after implanting the inner surface of the carotid bifurcation model (magnification × 100).
Mentions: Semipermeable poly tetra fluor ethylene (PTFE) was employed to create the carotid bifurcation model. The inner diameter of each region of a human carotid artery was measured by Doppler ultrasound and computerized tomography angiography. Because a previous model at 1:1 resulted in uneven endothelialization and other technical difficulties, these measurements were amplified at a rate of 1:1.5 (Table 1) experimentally to create a bifurcation model of the carotid artery that was subsequently coated with endothelial cells on the inner wall of the model to induce the endothelialization of the inner wall of the model as shown in Figure 1 [26].

Bottom Line: HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus.With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased.The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, West China Medical Center, Sichuan University, Chengdu, China.

ABSTRACT
The purpose of this study was to determine the concentration polarization of high-density lipoprotein (HDL) at the surface of the carotid artery under conditions of steady flow and to establish its relationship with shear stress using an in vitro vascular simulation model of carotid bifurcation. Shear stress, HDL concentration at the surface, and the ratio of HDL concentration at the surface to concentration in bulk flow were measured at different locations within the model under high-speed (1.451 m/s) and low-speed (0.559 m/s) flow. HDL showed concentration polarization at the surface of the carotid artery model, particularly in the internal carotid artery sinus. With decreasing flow velocity, the shear stress at the surface also decreased, and HDL concentration polarization increased. The concentration polarization of HDL was negatively and strongly correlated with shear stress at both low- (r = -0.872, P < .001) and high-speed flow (r = -0.592, P = .0018).

Show MeSH