Limits...
Rational design of improved pharmabiotics.

Sleator RD, Hill C - J. Biomed. Biotechnol. (2009)

Bottom Line: Herein we review the most recent advances in probiotic research and applications with particular emphasis on the novel concept of patho-biotechnology: the application of pathogen-derived (ex vivo and in vivo) stress survival strategies for the design of more technologically robust and effective probiotic cultures with improved biotechnological and clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Alimentary Pharmabiotic Centre, University College Cork, Ireland. roy.sleator@cit.ie

ABSTRACT
Herein we review the most recent advances in probiotic research and applications with particular emphasis on the novel concept of patho-biotechnology: the application of pathogen-derived (ex vivo and in vivo) stress survival strategies for the design of more technologically robust and effective probiotic cultures with improved biotechnological and clinical applications.

Show MeSH
The patho-biotechnology concept involves three approaches to generating improved probiotic cultures, adapted from Sleator and Hill [19].
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2742647&req=5

fig1: The patho-biotechnology concept involves three approaches to generating improved probiotic cultures, adapted from Sleator and Hill [19].

Mentions: The patho-biotechnology concept [5–7] seeks to attain this goal, ultimately leading to the development of improved probiotic strains. A primary focus of this approach involves equipping probiotic bacteria with the genetic elements necessary to overcome the many stresses encountered during the probiotic life cycle (both external and internal to the host) as well as enabling probiotics to better deal with invading pathogens [8, 9]. This strategy can be divided into three distinct approaches (Figure 1). The first tackles the issue of probiotic storage and delivery by cloning and expression of pathogen specific stress survival mechanisms (facilitating improved survival at extremes of temperature and water availability), thus countering reductions in probiotic numbers which can occur during manufacture and storage of delivery matrices (such as foods and tablet formulations). The second approach aims to improve host persistence by expression of host specific survival strategies (or virulence associated factors—such as the ability to cope with bile; an important component of the bodies physicochemical defence system) thereby positively affecting the therapeutic efficacy of the probiotic. The final approach involves the development of the so-called “designer probiotics;” strains which specifically target invading pathogens by blocking crucial ligand-receptor interactions between the pathogen and host cell [10].


Rational design of improved pharmabiotics.

Sleator RD, Hill C - J. Biomed. Biotechnol. (2009)

The patho-biotechnology concept involves three approaches to generating improved probiotic cultures, adapted from Sleator and Hill [19].
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2742647&req=5

fig1: The patho-biotechnology concept involves three approaches to generating improved probiotic cultures, adapted from Sleator and Hill [19].
Mentions: The patho-biotechnology concept [5–7] seeks to attain this goal, ultimately leading to the development of improved probiotic strains. A primary focus of this approach involves equipping probiotic bacteria with the genetic elements necessary to overcome the many stresses encountered during the probiotic life cycle (both external and internal to the host) as well as enabling probiotics to better deal with invading pathogens [8, 9]. This strategy can be divided into three distinct approaches (Figure 1). The first tackles the issue of probiotic storage and delivery by cloning and expression of pathogen specific stress survival mechanisms (facilitating improved survival at extremes of temperature and water availability), thus countering reductions in probiotic numbers which can occur during manufacture and storage of delivery matrices (such as foods and tablet formulations). The second approach aims to improve host persistence by expression of host specific survival strategies (or virulence associated factors—such as the ability to cope with bile; an important component of the bodies physicochemical defence system) thereby positively affecting the therapeutic efficacy of the probiotic. The final approach involves the development of the so-called “designer probiotics;” strains which specifically target invading pathogens by blocking crucial ligand-receptor interactions between the pathogen and host cell [10].

Bottom Line: Herein we review the most recent advances in probiotic research and applications with particular emphasis on the novel concept of patho-biotechnology: the application of pathogen-derived (ex vivo and in vivo) stress survival strategies for the design of more technologically robust and effective probiotic cultures with improved biotechnological and clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Alimentary Pharmabiotic Centre, University College Cork, Ireland. roy.sleator@cit.ie

ABSTRACT
Herein we review the most recent advances in probiotic research and applications with particular emphasis on the novel concept of patho-biotechnology: the application of pathogen-derived (ex vivo and in vivo) stress survival strategies for the design of more technologically robust and effective probiotic cultures with improved biotechnological and clinical applications.

Show MeSH