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DNA copy number changes in young gastric cancer patients with special reference to chromosome 19.

Varis A, van Rees B, Weterman M, Ristimäki A, Offerhaus J, Knuutila S - Br. J. Cancer (2003)

Bottom Line: To confirm the CGH results and to characterise the amplicon region on the most frequently amplified chromosome, chromosome 19, we carried out fluorescence in situ hybridisation (FISH) analysis and Southern blot analysis.Fluorescence in situ hybridisation with the bacterial artificial chromosome (BAC) clone mapped to 19q12 indicated a copy number increase in all eight tumour specimens studied.Cyclin E protein overexpression was verified in tumours with amplification on chromosome 19.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pathology and Medical Genetics, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, POB 400 (Haartmaninkatu 3, 4th floor), FIN-00029 HUS, Helsinki, Finland.

ABSTRACT
Only a few cytogenetic and genetic studies have been performed in gastric cancer patients in young age groups. In the present study we used the comparative genomic hybridisation (CGH) method to characterise frequent DNA copy number changes in 22 gastric cancer patients of 45 years or younger and three gastric cancer cell lines established from patients younger than 45 years. Analysis of DNA copy number changes revealed frequent DNA copy number increases at chromosomes 17q (52%), 19q (68%) and 20q (64%). To confirm the CGH results and to characterise the amplicon region on the most frequently amplified chromosome, chromosome 19, we carried out fluorescence in situ hybridisation (FISH) analysis and Southern blot analysis. Fluorescence in situ hybridisation with the bacterial artificial chromosome (BAC) clone mapped to 19q12 indicated a copy number increase in all eight tumour specimens studied. Southern blot analysis of six tumour specimens and three tumour cell lines, with five probes mapped to the 19q12-13.2 region, suggested cyclin E to be one of the candidate target genes in the 19q region for gastric cancer tumorigenesis. Cyclin E protein overexpression was verified in tumours with amplification on chromosome 19. Further studies are required to investigate the biological and clinical significance of 19q amplicon and cyclin E upregulation in gastric cancer of young patients.

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Immunohistochemical analysis of cyclin E protein expression in gastric cancer tumours. (A) Diffuse type of gastric cancer tumour without 19q amplicon, no detection of cyclin E overexpression and (B) Intestinal type of gastric cancer with 19q amplicon, increased nuclear staining of cyclin E detected.
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fig2: Immunohistochemical analysis of cyclin E protein expression in gastric cancer tumours. (A) Diffuse type of gastric cancer tumour without 19q amplicon, no detection of cyclin E overexpression and (B) Intestinal type of gastric cancer with 19q amplicon, increased nuclear staining of cyclin E detected.

Mentions: Fluorescence in situ hybridisation and immunohistochemical analysis of intestinal type of gastric cancer tumour (Case No. 10). (A) Fluorescence in situ hybridisation image of hybridisation with BAC probe targeting the 19q12 region (green signals). Normal nucleus with two signals on the left side and nucleus with amplification on the right side. (B) The same tumour indicating positive staining of cyclin E protein.


DNA copy number changes in young gastric cancer patients with special reference to chromosome 19.

Varis A, van Rees B, Weterman M, Ristimäki A, Offerhaus J, Knuutila S - Br. J. Cancer (2003)

Immunohistochemical analysis of cyclin E protein expression in gastric cancer tumours. (A) Diffuse type of gastric cancer tumour without 19q amplicon, no detection of cyclin E overexpression and (B) Intestinal type of gastric cancer with 19q amplicon, increased nuclear staining of cyclin E detected.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2741104&req=5

fig2: Immunohistochemical analysis of cyclin E protein expression in gastric cancer tumours. (A) Diffuse type of gastric cancer tumour without 19q amplicon, no detection of cyclin E overexpression and (B) Intestinal type of gastric cancer with 19q amplicon, increased nuclear staining of cyclin E detected.
Mentions: Fluorescence in situ hybridisation and immunohistochemical analysis of intestinal type of gastric cancer tumour (Case No. 10). (A) Fluorescence in situ hybridisation image of hybridisation with BAC probe targeting the 19q12 region (green signals). Normal nucleus with two signals on the left side and nucleus with amplification on the right side. (B) The same tumour indicating positive staining of cyclin E protein.

Bottom Line: To confirm the CGH results and to characterise the amplicon region on the most frequently amplified chromosome, chromosome 19, we carried out fluorescence in situ hybridisation (FISH) analysis and Southern blot analysis.Fluorescence in situ hybridisation with the bacterial artificial chromosome (BAC) clone mapped to 19q12 indicated a copy number increase in all eight tumour specimens studied.Cyclin E protein overexpression was verified in tumours with amplification on chromosome 19.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pathology and Medical Genetics, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, POB 400 (Haartmaninkatu 3, 4th floor), FIN-00029 HUS, Helsinki, Finland.

ABSTRACT
Only a few cytogenetic and genetic studies have been performed in gastric cancer patients in young age groups. In the present study we used the comparative genomic hybridisation (CGH) method to characterise frequent DNA copy number changes in 22 gastric cancer patients of 45 years or younger and three gastric cancer cell lines established from patients younger than 45 years. Analysis of DNA copy number changes revealed frequent DNA copy number increases at chromosomes 17q (52%), 19q (68%) and 20q (64%). To confirm the CGH results and to characterise the amplicon region on the most frequently amplified chromosome, chromosome 19, we carried out fluorescence in situ hybridisation (FISH) analysis and Southern blot analysis. Fluorescence in situ hybridisation with the bacterial artificial chromosome (BAC) clone mapped to 19q12 indicated a copy number increase in all eight tumour specimens studied. Southern blot analysis of six tumour specimens and three tumour cell lines, with five probes mapped to the 19q12-13.2 region, suggested cyclin E to be one of the candidate target genes in the 19q region for gastric cancer tumorigenesis. Cyclin E protein overexpression was verified in tumours with amplification on chromosome 19. Further studies are required to investigate the biological and clinical significance of 19q amplicon and cyclin E upregulation in gastric cancer of young patients.

Show MeSH
Related in: MedlinePlus