Limits...
Expression of cyclin D1, D3, E, and p27 in human renal cell carcinoma analysed by tissue microarray.

Hedberg Y, Ljungberg B, Roos G, Landberg G - Br. J. Cancer (2003)

Bottom Line: In addition, conventional RCCs often expressed high cyclin D1 protein levels, while papillary RCCs exhibited high cyclin E.In summary, we have shown that G1/S regulatory defects are present in RCC and are associated with clinico-pathological parameters.The pattern of cell cycle regulatory defects also differed between RCC subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.

ABSTRACT
Aberrations in the G1/S transition of the cell cycle have been observed in many malignancies and seem to be critical in the transformation process. Few studies have delineated the presence of G1/S regulatory defects and their clinical relevance in renal cell carcinoma (RCC). Therefore, we have examined the protein contents of cyclin D1, D3, E, and p27 in 218 RCCs, using tissue microarray and immunohistochemistry. The results from a subset of tumours were confirmed by Western blotting and immunohistochemical staining of regular tissue sections. Interestingly, low protein contents of cyclin D1 and p27 were associated with high nuclear grade, large tumour size, and poor prognosis for patients with conventional tumours. We further observed substantial differences in the pattern of G1/S regulatory defects between the different RCC subtypes. The majority of both conventional and papillary cases expressed p27; however, chromophobe tumours generally lacked p27 staining. In addition, conventional RCCs often expressed high cyclin D1 protein levels, while papillary RCCs exhibited high cyclin E. In summary, we have shown that G1/S regulatory defects are present in RCC and are associated with clinico-pathological parameters. The pattern of cell cycle regulatory defects also differed between RCC subtypes.

Show MeSH

Related in: MedlinePlus

Comparison between TMA-based immunohistochemistry analysis of cyclin D1, D3, E, and p27 (1–4) with (A) Western-blot-based analysis (cyclin D1, D3, and E) or (B) immunohistochemistry of regular sections (p27).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2741051&req=5

fig3: Comparison between TMA-based immunohistochemistry analysis of cyclin D1, D3, E, and p27 (1–4) with (A) Western-blot-based analysis (cyclin D1, D3, and E) or (B) immunohistochemistry of regular sections (p27).

Mentions: Illustration of Western blot analysis of cyclin D1, D3, E, and actin in RCC. Actin was used as a loading control. The first lane, marked 9003 N, represents corresponding kidney cortex from patient 9003, whereas the tumour sample was loaded in lane 2. The other six lanes contain tumour samples to illustrate the variation in protein contents between the tumours.


Expression of cyclin D1, D3, E, and p27 in human renal cell carcinoma analysed by tissue microarray.

Hedberg Y, Ljungberg B, Roos G, Landberg G - Br. J. Cancer (2003)

Comparison between TMA-based immunohistochemistry analysis of cyclin D1, D3, E, and p27 (1–4) with (A) Western-blot-based analysis (cyclin D1, D3, and E) or (B) immunohistochemistry of regular sections (p27).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2741051&req=5

fig3: Comparison between TMA-based immunohistochemistry analysis of cyclin D1, D3, E, and p27 (1–4) with (A) Western-blot-based analysis (cyclin D1, D3, and E) or (B) immunohistochemistry of regular sections (p27).
Mentions: Illustration of Western blot analysis of cyclin D1, D3, E, and actin in RCC. Actin was used as a loading control. The first lane, marked 9003 N, represents corresponding kidney cortex from patient 9003, whereas the tumour sample was loaded in lane 2. The other six lanes contain tumour samples to illustrate the variation in protein contents between the tumours.

Bottom Line: In addition, conventional RCCs often expressed high cyclin D1 protein levels, while papillary RCCs exhibited high cyclin E.In summary, we have shown that G1/S regulatory defects are present in RCC and are associated with clinico-pathological parameters.The pattern of cell cycle regulatory defects also differed between RCC subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.

ABSTRACT
Aberrations in the G1/S transition of the cell cycle have been observed in many malignancies and seem to be critical in the transformation process. Few studies have delineated the presence of G1/S regulatory defects and their clinical relevance in renal cell carcinoma (RCC). Therefore, we have examined the protein contents of cyclin D1, D3, E, and p27 in 218 RCCs, using tissue microarray and immunohistochemistry. The results from a subset of tumours were confirmed by Western blotting and immunohistochemical staining of regular tissue sections. Interestingly, low protein contents of cyclin D1 and p27 were associated with high nuclear grade, large tumour size, and poor prognosis for patients with conventional tumours. We further observed substantial differences in the pattern of G1/S regulatory defects between the different RCC subtypes. The majority of both conventional and papillary cases expressed p27; however, chromophobe tumours generally lacked p27 staining. In addition, conventional RCCs often expressed high cyclin D1 protein levels, while papillary RCCs exhibited high cyclin E. In summary, we have shown that G1/S regulatory defects are present in RCC and are associated with clinico-pathological parameters. The pattern of cell cycle regulatory defects also differed between RCC subtypes.

Show MeSH
Related in: MedlinePlus