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Expression of cyclin D1, D3, E, and p27 in human renal cell carcinoma analysed by tissue microarray.

Hedberg Y, Ljungberg B, Roos G, Landberg G - Br. J. Cancer (2003)

Bottom Line: In addition, conventional RCCs often expressed high cyclin D1 protein levels, while papillary RCCs exhibited high cyclin E.In summary, we have shown that G1/S regulatory defects are present in RCC and are associated with clinico-pathological parameters.The pattern of cell cycle regulatory defects also differed between RCC subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.

ABSTRACT
Aberrations in the G1/S transition of the cell cycle have been observed in many malignancies and seem to be critical in the transformation process. Few studies have delineated the presence of G1/S regulatory defects and their clinical relevance in renal cell carcinoma (RCC). Therefore, we have examined the protein contents of cyclin D1, D3, E, and p27 in 218 RCCs, using tissue microarray and immunohistochemistry. The results from a subset of tumours were confirmed by Western blotting and immunohistochemical staining of regular tissue sections. Interestingly, low protein contents of cyclin D1 and p27 were associated with high nuclear grade, large tumour size, and poor prognosis for patients with conventional tumours. We further observed substantial differences in the pattern of G1/S regulatory defects between the different RCC subtypes. The majority of both conventional and papillary cases expressed p27; however, chromophobe tumours generally lacked p27 staining. In addition, conventional RCCs often expressed high cyclin D1 protein levels, while papillary RCCs exhibited high cyclin E. In summary, we have shown that G1/S regulatory defects are present in RCC and are associated with clinico-pathological parameters. The pattern of cell cycle regulatory defects also differed between RCC subtypes.

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Related in: MedlinePlus

Illustration of cyclin D1, D3, E, and p27 immunohistochemistry staining of RCC using TMA technique. The left column illustrates a low protein expression, and the right column shows tumours with high content of the cell-cycle-regulating proteins.
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fig1: Illustration of cyclin D1, D3, E, and p27 immunohistochemistry staining of RCC using TMA technique. The left column illustrates a low protein expression, and the right column shows tumours with high content of the cell-cycle-regulating proteins.

Mentions: Examples of immunohistochemistry staining of cyclin D1, D3, E, and p27 are shown in Figure 1Figure 1


Expression of cyclin D1, D3, E, and p27 in human renal cell carcinoma analysed by tissue microarray.

Hedberg Y, Ljungberg B, Roos G, Landberg G - Br. J. Cancer (2003)

Illustration of cyclin D1, D3, E, and p27 immunohistochemistry staining of RCC using TMA technique. The left column illustrates a low protein expression, and the right column shows tumours with high content of the cell-cycle-regulating proteins.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2741051&req=5

fig1: Illustration of cyclin D1, D3, E, and p27 immunohistochemistry staining of RCC using TMA technique. The left column illustrates a low protein expression, and the right column shows tumours with high content of the cell-cycle-regulating proteins.
Mentions: Examples of immunohistochemistry staining of cyclin D1, D3, E, and p27 are shown in Figure 1Figure 1

Bottom Line: In addition, conventional RCCs often expressed high cyclin D1 protein levels, while papillary RCCs exhibited high cyclin E.In summary, we have shown that G1/S regulatory defects are present in RCC and are associated with clinico-pathological parameters.The pattern of cell cycle regulatory defects also differed between RCC subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.

ABSTRACT
Aberrations in the G1/S transition of the cell cycle have been observed in many malignancies and seem to be critical in the transformation process. Few studies have delineated the presence of G1/S regulatory defects and their clinical relevance in renal cell carcinoma (RCC). Therefore, we have examined the protein contents of cyclin D1, D3, E, and p27 in 218 RCCs, using tissue microarray and immunohistochemistry. The results from a subset of tumours were confirmed by Western blotting and immunohistochemical staining of regular tissue sections. Interestingly, low protein contents of cyclin D1 and p27 were associated with high nuclear grade, large tumour size, and poor prognosis for patients with conventional tumours. We further observed substantial differences in the pattern of G1/S regulatory defects between the different RCC subtypes. The majority of both conventional and papillary cases expressed p27; however, chromophobe tumours generally lacked p27 staining. In addition, conventional RCCs often expressed high cyclin D1 protein levels, while papillary RCCs exhibited high cyclin E. In summary, we have shown that G1/S regulatory defects are present in RCC and are associated with clinico-pathological parameters. The pattern of cell cycle regulatory defects also differed between RCC subtypes.

Show MeSH
Related in: MedlinePlus