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Prenatal and early, but not late, postnatal exposure of mice to sidestream tobacco smoke increases airway hyperresponsiveness later in life.

Wu ZX, Hunter DD, Kish VL, Benders KM, Batchelor TP, Dey RD - Environ. Health Perspect. (2009)

Bottom Line: Methacholine (MCh) dose response for lung resistance (R(L)) was significantly elevated, and dynamic pulmonary compliance (C(dyn)) was significantly decreased, in the GD7 and PND2 SS exposure groups compared with the FA groups after SS exposure on PND59.At the same time points, the percent area of SP nerve fibers in tracheal smooth muscle and the levels of NGF were significantly elevated.MCh dose-response curves for R(L) and C(dyn), SP nerve fiber density, and the level of NGF were not significantly changed in the PND21 exposure group after SS exposure on PND59.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurobiology and Anatomy, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV 26506, USA. zwu@hsc.wvu.edu

ABSTRACT

Background: Cigarette smoke exposure in utero and during early postnatal development increases the incidence of asthma and airway hyperresponsiveness (AHR) later in life, suggesting that a possible critical period of developmental sensitivity exists in the prenatal and early postnatal periods.

Objective: We investigated mechanisms of susceptibility during critical developmental periods to sidestream smoke (SS) exposure and evaluated the possible effects of SS on neural responses.

Methods: We exposed three different age groups of mice to either SS or filtered air (FA) for 10 consecutive days beginning on gestation day (GD) 7 by maternal exposure or beginning on postnatal day (PND) 2 or PND21 by direct inhalation. Lung function, airway substance P (SP) innervation, and nerve growth factor (NGF) levels in broncho alveolar lavage fluid were measured after a single SS exposure on PND59.

Results: Methacholine (MCh) dose response for lung resistance (R(L)) was significantly elevated, and dynamic pulmonary compliance (C(dyn)) was significantly decreased, in the GD7 and PND2 SS exposure groups compared with the FA groups after SS exposure on PND59. At the same time points, the percent area of SP nerve fibers in tracheal smooth muscle and the levels of NGF were significantly elevated. MCh dose-response curves for R(L) and C(dyn), SP nerve fiber density, and the level of NGF were not significantly changed in the PND21 exposure group after SS exposure on PND59.

Conclusions: These results suggest that a critical period of susceptibility to SS exposure exists in the prenatal and early postnatal period of development in mice that results in increased SP innervation, increased NGF levels in the airway, and enhanced MCh AHR later in life.

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Related in: MedlinePlus

Levels of NGF release in BALF in GD7 (A), PND2 (B), and PND21 (C) FA- or SS-exposed mice after SS exposure on PND59. Data are mean ± SE of 6/group. NGF was measured by ELISA.*Significant difference between FA- and SS-exposed mice (p ≤ 0.05).
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f6-ehp-117-1434: Levels of NGF release in BALF in GD7 (A), PND2 (B), and PND21 (C) FA- or SS-exposed mice after SS exposure on PND59. Data are mean ± SE of 6/group. NGF was measured by ELISA.*Significant difference between FA- and SS-exposed mice (p ≤ 0.05).

Mentions: Thee concentrations of NGF in BALF were significantly higher in SS- versus FA-exposed mice in the GD7 and PND2 exposure groups 16 hr after SS exposure on PND59 (95% CI, 167–375 vs. 23–143 pg/mL and 214–450 vs. 58–180 pg/mL, respectively). At the same time, the level of NGF was not significantly different in SS versus FA mice in the PND21 group (95% CI, 70–234 vs. 24–166 pg/mL; Figure 6).


Prenatal and early, but not late, postnatal exposure of mice to sidestream tobacco smoke increases airway hyperresponsiveness later in life.

Wu ZX, Hunter DD, Kish VL, Benders KM, Batchelor TP, Dey RD - Environ. Health Perspect. (2009)

Levels of NGF release in BALF in GD7 (A), PND2 (B), and PND21 (C) FA- or SS-exposed mice after SS exposure on PND59. Data are mean ± SE of 6/group. NGF was measured by ELISA.*Significant difference between FA- and SS-exposed mice (p ≤ 0.05).
© Copyright Policy - public-domain
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2737022&req=5

f6-ehp-117-1434: Levels of NGF release in BALF in GD7 (A), PND2 (B), and PND21 (C) FA- or SS-exposed mice after SS exposure on PND59. Data are mean ± SE of 6/group. NGF was measured by ELISA.*Significant difference between FA- and SS-exposed mice (p ≤ 0.05).
Mentions: Thee concentrations of NGF in BALF were significantly higher in SS- versus FA-exposed mice in the GD7 and PND2 exposure groups 16 hr after SS exposure on PND59 (95% CI, 167–375 vs. 23–143 pg/mL and 214–450 vs. 58–180 pg/mL, respectively). At the same time, the level of NGF was not significantly different in SS versus FA mice in the PND21 group (95% CI, 70–234 vs. 24–166 pg/mL; Figure 6).

Bottom Line: Methacholine (MCh) dose response for lung resistance (R(L)) was significantly elevated, and dynamic pulmonary compliance (C(dyn)) was significantly decreased, in the GD7 and PND2 SS exposure groups compared with the FA groups after SS exposure on PND59.At the same time points, the percent area of SP nerve fibers in tracheal smooth muscle and the levels of NGF were significantly elevated.MCh dose-response curves for R(L) and C(dyn), SP nerve fiber density, and the level of NGF were not significantly changed in the PND21 exposure group after SS exposure on PND59.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurobiology and Anatomy, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV 26506, USA. zwu@hsc.wvu.edu

ABSTRACT

Background: Cigarette smoke exposure in utero and during early postnatal development increases the incidence of asthma and airway hyperresponsiveness (AHR) later in life, suggesting that a possible critical period of developmental sensitivity exists in the prenatal and early postnatal periods.

Objective: We investigated mechanisms of susceptibility during critical developmental periods to sidestream smoke (SS) exposure and evaluated the possible effects of SS on neural responses.

Methods: We exposed three different age groups of mice to either SS or filtered air (FA) for 10 consecutive days beginning on gestation day (GD) 7 by maternal exposure or beginning on postnatal day (PND) 2 or PND21 by direct inhalation. Lung function, airway substance P (SP) innervation, and nerve growth factor (NGF) levels in broncho alveolar lavage fluid were measured after a single SS exposure on PND59.

Results: Methacholine (MCh) dose response for lung resistance (R(L)) was significantly elevated, and dynamic pulmonary compliance (C(dyn)) was significantly decreased, in the GD7 and PND2 SS exposure groups compared with the FA groups after SS exposure on PND59. At the same time points, the percent area of SP nerve fibers in tracheal smooth muscle and the levels of NGF were significantly elevated. MCh dose-response curves for R(L) and C(dyn), SP nerve fiber density, and the level of NGF were not significantly changed in the PND21 exposure group after SS exposure on PND59.

Conclusions: These results suggest that a critical period of susceptibility to SS exposure exists in the prenatal and early postnatal period of development in mice that results in increased SP innervation, increased NGF levels in the airway, and enhanced MCh AHR later in life.

Show MeSH
Related in: MedlinePlus