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AtTRP1 encodes a novel TPR protein that interacts with the ethylene receptor ERS1 and modulates development in Arabidopsis.

Lin Z, Ho CW, Grierson D - J. Exp. Bot. (2009)

Bottom Line: This association was confirmed by in vivo co-immunoprecipitation.Plants overexpressing AtTRP1 also showed a reduced response to exogenous IAA and altered expression of a subset of auxin early responsive genes.A model for AtTRP1 action is proposed.

View Article: PubMed Central - PubMed

Affiliation: Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK.

ABSTRACT
Arabidopsis AtTRP1 is an orthologue of SlTPR1, a tomato tetratricopeptide repeat protein that interacts with the tomato ethylene receptors LeETR1 and NR in yeast 2-hybrid assays and in vitro, and modulates plant development. AtTRP1 is encoded by a single copy gene in the Arabidopsis genome, and is related to TCC1, a human protein that competes with Raf-1 for Ras binding, and distantly related to the immunophilin-like FK-binding proteins TWD1 and PAS1. The former is involved in auxin transport and the latter is translocated to the nucleus in response to auxin. AtTRP1 interacted preferentially with the Arabidopsis ethylene receptor ERS1 in yeast two-hybrid assays. This association was confirmed by in vivo co-immunoprecipitation. AtTRP1 promoter-GUS was highly expressed in vascular tissue, mature anthers, the abscission zone, and was induced by ACC. Overexpression of AtTRP1 in wild-type Arabidopsis resulted in dwarf plants with reduced fertility, altered leaf/silique morphology, and enhanced expression of the ethylene responsive gene AtChitB. Exogenous GA did not reverse the dwarf habit. Etiolated transgenic seedlings overexpressing AtTRP1 displayed enhanced sensitivity to low ACC and this was correlated with the transgene expression. Seedlings overexpressing AtTRP1 at high levels exhibited shortened and swollen hypocotyls, inhibited root growth, and an altered apical hook. Plants overexpressing AtTRP1 also showed a reduced response to exogenous IAA and altered expression of a subset of auxin early responsive genes. These results indicated that overexpression of AtTRP1 affects cross-talk between ethylene and auxin signalling and enhances some ethylene responses and alters some auxin responses. A model for AtTRP1 action is proposed.

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Expression pattern of AtTRP1 by Northern analysis. RNA samples were isolated from a range of tissues at different developmental stages from wild-type plants (Columbia). YB, unopened flower buds; OB, flower buds starting to open; OF, fully opened flowers; SF, senescing flowers; YS, young siliques; MS, mature siliques; SS, senescing siliques; ST, stems; EL, emerging leaves; ML, fully expanded leaves; SL, senescing leaves. 10 μg total RNA was used for the electrophoresis and blotting and the full-length AtTRP1 cDNA was used as probe. The ethidium bromide-stained rRNA gel below indicates the sample loading.
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fig3: Expression pattern of AtTRP1 by Northern analysis. RNA samples were isolated from a range of tissues at different developmental stages from wild-type plants (Columbia). YB, unopened flower buds; OB, flower buds starting to open; OF, fully opened flowers; SF, senescing flowers; YS, young siliques; MS, mature siliques; SS, senescing siliques; ST, stems; EL, emerging leaves; ML, fully expanded leaves; SL, senescing leaves. 10 μg total RNA was used for the electrophoresis and blotting and the full-length AtTRP1 cDNA was used as probe. The ethidium bromide-stained rRNA gel below indicates the sample loading.

Mentions: Northern analysis using the full-length AtTRP1 cDNA as probe indicated that AtTRP1 mRNA was consistently highly expressed in all stages of developing flowers, i.e. unopened, early-opened, fully-opened, and senescing flowers, stems, and leaves (including emerging, mature, and senescing leaves), but was absent or present at a low level in siliques (Fig. 3).


AtTRP1 encodes a novel TPR protein that interacts with the ethylene receptor ERS1 and modulates development in Arabidopsis.

Lin Z, Ho CW, Grierson D - J. Exp. Bot. (2009)

Expression pattern of AtTRP1 by Northern analysis. RNA samples were isolated from a range of tissues at different developmental stages from wild-type plants (Columbia). YB, unopened flower buds; OB, flower buds starting to open; OF, fully opened flowers; SF, senescing flowers; YS, young siliques; MS, mature siliques; SS, senescing siliques; ST, stems; EL, emerging leaves; ML, fully expanded leaves; SL, senescing leaves. 10 μg total RNA was used for the electrophoresis and blotting and the full-length AtTRP1 cDNA was used as probe. The ethidium bromide-stained rRNA gel below indicates the sample loading.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2736885&req=5

fig3: Expression pattern of AtTRP1 by Northern analysis. RNA samples were isolated from a range of tissues at different developmental stages from wild-type plants (Columbia). YB, unopened flower buds; OB, flower buds starting to open; OF, fully opened flowers; SF, senescing flowers; YS, young siliques; MS, mature siliques; SS, senescing siliques; ST, stems; EL, emerging leaves; ML, fully expanded leaves; SL, senescing leaves. 10 μg total RNA was used for the electrophoresis and blotting and the full-length AtTRP1 cDNA was used as probe. The ethidium bromide-stained rRNA gel below indicates the sample loading.
Mentions: Northern analysis using the full-length AtTRP1 cDNA as probe indicated that AtTRP1 mRNA was consistently highly expressed in all stages of developing flowers, i.e. unopened, early-opened, fully-opened, and senescing flowers, stems, and leaves (including emerging, mature, and senescing leaves), but was absent or present at a low level in siliques (Fig. 3).

Bottom Line: This association was confirmed by in vivo co-immunoprecipitation.Plants overexpressing AtTRP1 also showed a reduced response to exogenous IAA and altered expression of a subset of auxin early responsive genes.A model for AtTRP1 action is proposed.

View Article: PubMed Central - PubMed

Affiliation: Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK.

ABSTRACT
Arabidopsis AtTRP1 is an orthologue of SlTPR1, a tomato tetratricopeptide repeat protein that interacts with the tomato ethylene receptors LeETR1 and NR in yeast 2-hybrid assays and in vitro, and modulates plant development. AtTRP1 is encoded by a single copy gene in the Arabidopsis genome, and is related to TCC1, a human protein that competes with Raf-1 for Ras binding, and distantly related to the immunophilin-like FK-binding proteins TWD1 and PAS1. The former is involved in auxin transport and the latter is translocated to the nucleus in response to auxin. AtTRP1 interacted preferentially with the Arabidopsis ethylene receptor ERS1 in yeast two-hybrid assays. This association was confirmed by in vivo co-immunoprecipitation. AtTRP1 promoter-GUS was highly expressed in vascular tissue, mature anthers, the abscission zone, and was induced by ACC. Overexpression of AtTRP1 in wild-type Arabidopsis resulted in dwarf plants with reduced fertility, altered leaf/silique morphology, and enhanced expression of the ethylene responsive gene AtChitB. Exogenous GA did not reverse the dwarf habit. Etiolated transgenic seedlings overexpressing AtTRP1 displayed enhanced sensitivity to low ACC and this was correlated with the transgene expression. Seedlings overexpressing AtTRP1 at high levels exhibited shortened and swollen hypocotyls, inhibited root growth, and an altered apical hook. Plants overexpressing AtTRP1 also showed a reduced response to exogenous IAA and altered expression of a subset of auxin early responsive genes. These results indicated that overexpression of AtTRP1 affects cross-talk between ethylene and auxin signalling and enhances some ethylene responses and alters some auxin responses. A model for AtTRP1 action is proposed.

Show MeSH
Related in: MedlinePlus