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Effects of Lansoprazole on the Lipopolysaccharide-Stimulated Toll-Like Receptor 4 Signal Transduction Systems: A Study Using the 293hTLR4/MD2-CD14 Cells.

Ohara T, Kanoh Y, Yoshino K, Kitajima M - J Clin Biochem Nutr (2009)

Bottom Line: The present study was undertaken to evaluate the effects of lansoprazole (LPZ) on lipopolysaccharide (LPS)-stimulated toll-like receptor 4 (TLR4) signal transduction systems using the 293hTLR4/MD2-CD14 cells.The cells were incubated and then divided into the following groups: (a) untreated group, (b) non-LPZ treated (1h) group, (c) LPZ-treated (1h) plus non LPS-stimulated (1h) group, (d) LPZ-treated (1h) plus non LPS-stimulated (6h) group, (e) LPZ-treated (1h) plus LPS-stimulated (1h) group, (f) LPZ-treated (1h) plus LPS-stimulated (6h) group, (g) non LPZ-treated (1h) plus LPS-stimulated (1h) group and (h) non LPZ-treated (1h) plus LPS-stimulated (6h) group.Samples from each group were subjected to western blotting for analysis of IkB phosphorylation, intranuclear transfer of NF-kB, phosphorylation of MAP kinase (MAPK), intranuclear transfer of interferon regulatory factor 5 (IRF5), and expression of suppressor of cytokine signaling-1 (SOCS1).

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, International University of Health and Welfare Hospital, 537-3 Iguchi, Nasushiobara, Tochigi 329-2763, Japan.

ABSTRACT
The present study was undertaken to evaluate the effects of lansoprazole (LPZ) on lipopolysaccharide (LPS)-stimulated toll-like receptor 4 (TLR4) signal transduction systems using the 293hTLR4/MD2-CD14 cells. The cells were incubated and then divided into the following groups: (a) untreated group, (b) non-LPZ treated (1h) group, (c) LPZ-treated (1h) plus non LPS-stimulated (1h) group, (d) LPZ-treated (1h) plus non LPS-stimulated (6h) group, (e) LPZ-treated (1h) plus LPS-stimulated (1h) group, (f) LPZ-treated (1h) plus LPS-stimulated (6h) group, (g) non LPZ-treated (1h) plus LPS-stimulated (1h) group and (h) non LPZ-treated (1h) plus LPS-stimulated (6h) group. Samples from each group were subjected to western blotting for analysis of IkB phosphorylation, intranuclear transfer of NF-kB, phosphorylation of MAP kinase (MAPK), intranuclear transfer of interferon regulatory factor 5 (IRF5), and expression of suppressor of cytokine signaling-1 (SOCS1). In the LPZ-treated groups, neither phosphorylation of MAPK nor intranuclear transfer of IRF5 was suppressed under stimulation with LPS, and enhanced intranuclear transfer of NF-kB and increased expression of SOCS1 were noted by comparison with the group treated with LPS alone. These results suggest that LPZ stimulates the expression of SOCS1 and regulates protein phosphorylation through its activity on TLR4 signal transduction under LPS stimulation.

No MeSH data available.


The phosphorylation of p38 (phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody) analyzed by western-blotting. An arrow indicates the induction of phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody, respectively.
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Figure 4: The phosphorylation of p38 (phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody) analyzed by western-blotting. An arrow indicates the induction of phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody, respectively.

Mentions: In the LPS-stimulated group, the phosphorylation of IkB, phospho-p44/42 MAPK antibody and p44/42 MAPK antibody, phospho-SAPK/JNK (Thr183/Tyr185) antibody) and phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody was suppressed after 6 h of stimulation as compared to that observed after 1h of stimulation (Figs. 1, 2, 3 and 4), and intranuclear transfer of IFR5 and NF-kB was noted (Figs. 5 and 6). In the LPZ-treated group, the phosphorylation of IkB, phospho-p44/42 MAPK antibody and p44/42, phospho-SAPK/JNK (Thr183/Tyr185) antibody) and phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody was not suppressed (Figs. 1, 2, 3 and 4), and intranuclear transfer of IFR5 was not suppressed under stimulation with LPS (Fig. 6). The induction of SAPK/JNK (56G8) rabbit mAb was yet presence in all groups (Fig. 3). And then in the LPZ-treated groups, enhanced intranuclear transfer of NF-kB and increased expression of SOCS1 were noted, as compared to the observations in the group stimulated with LPS alone (Fig. 7).


Effects of Lansoprazole on the Lipopolysaccharide-Stimulated Toll-Like Receptor 4 Signal Transduction Systems: A Study Using the 293hTLR4/MD2-CD14 Cells.

Ohara T, Kanoh Y, Yoshino K, Kitajima M - J Clin Biochem Nutr (2009)

The phosphorylation of p38 (phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody) analyzed by western-blotting. An arrow indicates the induction of phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2735639&req=5

Figure 4: The phosphorylation of p38 (phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody) analyzed by western-blotting. An arrow indicates the induction of phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody, respectively.
Mentions: In the LPS-stimulated group, the phosphorylation of IkB, phospho-p44/42 MAPK antibody and p44/42 MAPK antibody, phospho-SAPK/JNK (Thr183/Tyr185) antibody) and phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody was suppressed after 6 h of stimulation as compared to that observed after 1h of stimulation (Figs. 1, 2, 3 and 4), and intranuclear transfer of IFR5 and NF-kB was noted (Figs. 5 and 6). In the LPZ-treated group, the phosphorylation of IkB, phospho-p44/42 MAPK antibody and p44/42, phospho-SAPK/JNK (Thr183/Tyr185) antibody) and phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody was not suppressed (Figs. 1, 2, 3 and 4), and intranuclear transfer of IFR5 was not suppressed under stimulation with LPS (Fig. 6). The induction of SAPK/JNK (56G8) rabbit mAb was yet presence in all groups (Fig. 3). And then in the LPZ-treated groups, enhanced intranuclear transfer of NF-kB and increased expression of SOCS1 were noted, as compared to the observations in the group stimulated with LPS alone (Fig. 7).

Bottom Line: The present study was undertaken to evaluate the effects of lansoprazole (LPZ) on lipopolysaccharide (LPS)-stimulated toll-like receptor 4 (TLR4) signal transduction systems using the 293hTLR4/MD2-CD14 cells.The cells were incubated and then divided into the following groups: (a) untreated group, (b) non-LPZ treated (1h) group, (c) LPZ-treated (1h) plus non LPS-stimulated (1h) group, (d) LPZ-treated (1h) plus non LPS-stimulated (6h) group, (e) LPZ-treated (1h) plus LPS-stimulated (1h) group, (f) LPZ-treated (1h) plus LPS-stimulated (6h) group, (g) non LPZ-treated (1h) plus LPS-stimulated (1h) group and (h) non LPZ-treated (1h) plus LPS-stimulated (6h) group.Samples from each group were subjected to western blotting for analysis of IkB phosphorylation, intranuclear transfer of NF-kB, phosphorylation of MAP kinase (MAPK), intranuclear transfer of interferon regulatory factor 5 (IRF5), and expression of suppressor of cytokine signaling-1 (SOCS1).

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, International University of Health and Welfare Hospital, 537-3 Iguchi, Nasushiobara, Tochigi 329-2763, Japan.

ABSTRACT
The present study was undertaken to evaluate the effects of lansoprazole (LPZ) on lipopolysaccharide (LPS)-stimulated toll-like receptor 4 (TLR4) signal transduction systems using the 293hTLR4/MD2-CD14 cells. The cells were incubated and then divided into the following groups: (a) untreated group, (b) non-LPZ treated (1h) group, (c) LPZ-treated (1h) plus non LPS-stimulated (1h) group, (d) LPZ-treated (1h) plus non LPS-stimulated (6h) group, (e) LPZ-treated (1h) plus LPS-stimulated (1h) group, (f) LPZ-treated (1h) plus LPS-stimulated (6h) group, (g) non LPZ-treated (1h) plus LPS-stimulated (1h) group and (h) non LPZ-treated (1h) plus LPS-stimulated (6h) group. Samples from each group were subjected to western blotting for analysis of IkB phosphorylation, intranuclear transfer of NF-kB, phosphorylation of MAP kinase (MAPK), intranuclear transfer of interferon regulatory factor 5 (IRF5), and expression of suppressor of cytokine signaling-1 (SOCS1). In the LPZ-treated groups, neither phosphorylation of MAPK nor intranuclear transfer of IRF5 was suppressed under stimulation with LPS, and enhanced intranuclear transfer of NF-kB and increased expression of SOCS1 were noted by comparison with the group treated with LPS alone. These results suggest that LPZ stimulates the expression of SOCS1 and regulates protein phosphorylation through its activity on TLR4 signal transduction under LPS stimulation.

No MeSH data available.