Limits...
Basic study on active changes in biological function of mouse liver graft in cold storage after low-dose x-irradiation.

Kataoka T, Yoshimoto M, Nakagawa S, Mizuguchi Y, Taguchi T, Yamaoka K - J Clin Biochem Nutr (2009)

Bottom Line: The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase.These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts.Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Okayama 700-8558, Japan.

ABSTRACT
We previously reported that low-dose X-irradiation alleviates ischemia-reperfusion injury such as mouse paw edema. In this study, we examined active changes in the biological function of mouse liver grafts in cold storage after low-dose X-irradiation. Mouse livers were sham-irradiated or were irradiated with 0.25, 0.5, 1.0, or 5.0 Gy of X-ray and stored for 4, 8, 24, or 48 h in preservation or saline solution. The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase. Following storage for 4, 8, or 48 h in preservation solution, lipid peroxide levels of the 0.5 Gy irradiated group were significantly lower than those of the sham irradiated group. Following storage for 24 h in preservation solution, the activity of SOD and catalase of the 1.0 Gy irradiated group were significantly higher than those of the sham irradiated group. Hepatocytes stored in saline solution were vacuolated. However, no vacuole formation was observed in hepatocytes stored in preservation solution. These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts. Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.

No MeSH data available.


Related in: MedlinePlus

Histological changes in mouse liver in preservation after irradiation. Mouse livers were examined histologically following preservation under the following conditions. A) Control, B) Cold storage for 4 h in physiological saline solution after sham irradiation, C) Cold storage for 24 h in preservation solution after sham irradiation, D) Cold storage for 24 h in physiological saline solution after sham irradiation, E) Cold storage for 48 h in preservation solution after sham irradiation, F) Cold storage for 48 h in physiological saline solution after sham irradiation, G) Cold storage for 24 h in preservation solution after 1.0 Gy irradiation, and H) Cold storage for 24 h in physiological saline solution after 1.0 Gy irradiation. The arrow shows vacuole formation in the liver. The length of the scale bar is 20 µm. For all figures HE staining was used.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2735636&req=5

Figure 6: Histological changes in mouse liver in preservation after irradiation. Mouse livers were examined histologically following preservation under the following conditions. A) Control, B) Cold storage for 4 h in physiological saline solution after sham irradiation, C) Cold storage for 24 h in preservation solution after sham irradiation, D) Cold storage for 24 h in physiological saline solution after sham irradiation, E) Cold storage for 48 h in preservation solution after sham irradiation, F) Cold storage for 48 h in physiological saline solution after sham irradiation, G) Cold storage for 24 h in preservation solution after 1.0 Gy irradiation, and H) Cold storage for 24 h in physiological saline solution after 1.0 Gy irradiation. The arrow shows vacuole formation in the liver. The length of the scale bar is 20 µm. For all figures HE staining was used.

Mentions: Histological changes in mouse livers following irradiation and storage in saline or preservation solution were examined. Histological examination of hepatocytes kept in cold storage in saline solution following irradiation indicated that the hepatocytes were vacuolated under these conditions. In contrast, no vacuole formation was observed in hepatocytes kept in cold storage in preservation solution following irradiation (Fig. 6).


Basic study on active changes in biological function of mouse liver graft in cold storage after low-dose x-irradiation.

Kataoka T, Yoshimoto M, Nakagawa S, Mizuguchi Y, Taguchi T, Yamaoka K - J Clin Biochem Nutr (2009)

Histological changes in mouse liver in preservation after irradiation. Mouse livers were examined histologically following preservation under the following conditions. A) Control, B) Cold storage for 4 h in physiological saline solution after sham irradiation, C) Cold storage for 24 h in preservation solution after sham irradiation, D) Cold storage for 24 h in physiological saline solution after sham irradiation, E) Cold storage for 48 h in preservation solution after sham irradiation, F) Cold storage for 48 h in physiological saline solution after sham irradiation, G) Cold storage for 24 h in preservation solution after 1.0 Gy irradiation, and H) Cold storage for 24 h in physiological saline solution after 1.0 Gy irradiation. The arrow shows vacuole formation in the liver. The length of the scale bar is 20 µm. For all figures HE staining was used.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2735636&req=5

Figure 6: Histological changes in mouse liver in preservation after irradiation. Mouse livers were examined histologically following preservation under the following conditions. A) Control, B) Cold storage for 4 h in physiological saline solution after sham irradiation, C) Cold storage for 24 h in preservation solution after sham irradiation, D) Cold storage for 24 h in physiological saline solution after sham irradiation, E) Cold storage for 48 h in preservation solution after sham irradiation, F) Cold storage for 48 h in physiological saline solution after sham irradiation, G) Cold storage for 24 h in preservation solution after 1.0 Gy irradiation, and H) Cold storage for 24 h in physiological saline solution after 1.0 Gy irradiation. The arrow shows vacuole formation in the liver. The length of the scale bar is 20 µm. For all figures HE staining was used.
Mentions: Histological changes in mouse livers following irradiation and storage in saline or preservation solution were examined. Histological examination of hepatocytes kept in cold storage in saline solution following irradiation indicated that the hepatocytes were vacuolated under these conditions. In contrast, no vacuole formation was observed in hepatocytes kept in cold storage in preservation solution following irradiation (Fig. 6).

Bottom Line: The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase.These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts.Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Okayama 700-8558, Japan.

ABSTRACT
We previously reported that low-dose X-irradiation alleviates ischemia-reperfusion injury such as mouse paw edema. In this study, we examined active changes in the biological function of mouse liver grafts in cold storage after low-dose X-irradiation. Mouse livers were sham-irradiated or were irradiated with 0.25, 0.5, 1.0, or 5.0 Gy of X-ray and stored for 4, 8, 24, or 48 h in preservation or saline solution. The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase. Following storage for 4, 8, or 48 h in preservation solution, lipid peroxide levels of the 0.5 Gy irradiated group were significantly lower than those of the sham irradiated group. Following storage for 24 h in preservation solution, the activity of SOD and catalase of the 1.0 Gy irradiated group were significantly higher than those of the sham irradiated group. Hepatocytes stored in saline solution were vacuolated. However, no vacuole formation was observed in hepatocytes stored in preservation solution. These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts. Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.

No MeSH data available.


Related in: MedlinePlus