Limits...
Basic study on active changes in biological function of mouse liver graft in cold storage after low-dose x-irradiation.

Kataoka T, Yoshimoto M, Nakagawa S, Mizuguchi Y, Taguchi T, Yamaoka K - J Clin Biochem Nutr (2009)

Bottom Line: The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase.These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts.Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Okayama 700-8558, Japan.

ABSTRACT
We previously reported that low-dose X-irradiation alleviates ischemia-reperfusion injury such as mouse paw edema. In this study, we examined active changes in the biological function of mouse liver grafts in cold storage after low-dose X-irradiation. Mouse livers were sham-irradiated or were irradiated with 0.25, 0.5, 1.0, or 5.0 Gy of X-ray and stored for 4, 8, 24, or 48 h in preservation or saline solution. The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase. Following storage for 4, 8, or 48 h in preservation solution, lipid peroxide levels of the 0.5 Gy irradiated group were significantly lower than those of the sham irradiated group. Following storage for 24 h in preservation solution, the activity of SOD and catalase of the 1.0 Gy irradiated group were significantly higher than those of the sham irradiated group. Hepatocytes stored in saline solution were vacuolated. However, no vacuole formation was observed in hepatocytes stored in preservation solution. These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts. Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.

No MeSH data available.


Related in: MedlinePlus

Changes in catalase activity of mouse liver in preservation after 0.5 Gy irradiation. Mouse livers were treated as described for Fig. 1 and catalase activity was measured by a spectrophotometric assay.The data, number of mouse livers and significance are as described Fig. 1.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2735636&req=5

Figure 4: Changes in catalase activity of mouse liver in preservation after 0.5 Gy irradiation. Mouse livers were treated as described for Fig. 1 and catalase activity was measured by a spectrophotometric assay.The data, number of mouse livers and significance are as described Fig. 1.

Mentions: Catalase activity of mouse livers kept in cold storage for 4 or 24 h in saline solution after 0.5 Gy irradiation was significantly increased. The catalase activities of mouse livers kept for 24 h in cold storage in saline solution after 0.5 Gy irradiation were significantly higher than those after sham irradiation (Fig. 4). These data indicate that parameters of oxidation in mouse livers are highly responsive to irradiation and storage conditions.


Basic study on active changes in biological function of mouse liver graft in cold storage after low-dose x-irradiation.

Kataoka T, Yoshimoto M, Nakagawa S, Mizuguchi Y, Taguchi T, Yamaoka K - J Clin Biochem Nutr (2009)

Changes in catalase activity of mouse liver in preservation after 0.5 Gy irradiation. Mouse livers were treated as described for Fig. 1 and catalase activity was measured by a spectrophotometric assay.The data, number of mouse livers and significance are as described Fig. 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2735636&req=5

Figure 4: Changes in catalase activity of mouse liver in preservation after 0.5 Gy irradiation. Mouse livers were treated as described for Fig. 1 and catalase activity was measured by a spectrophotometric assay.The data, number of mouse livers and significance are as described Fig. 1.
Mentions: Catalase activity of mouse livers kept in cold storage for 4 or 24 h in saline solution after 0.5 Gy irradiation was significantly increased. The catalase activities of mouse livers kept for 24 h in cold storage in saline solution after 0.5 Gy irradiation were significantly higher than those after sham irradiation (Fig. 4). These data indicate that parameters of oxidation in mouse livers are highly responsive to irradiation and storage conditions.

Bottom Line: The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase.These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts.Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Okayama 700-8558, Japan.

ABSTRACT
We previously reported that low-dose X-irradiation alleviates ischemia-reperfusion injury such as mouse paw edema. In this study, we examined active changes in the biological function of mouse liver grafts in cold storage after low-dose X-irradiation. Mouse livers were sham-irradiated or were irradiated with 0.25, 0.5, 1.0, or 5.0 Gy of X-ray and stored for 4, 8, 24, or 48 h in preservation or saline solution. The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase. Following storage for 4, 8, or 48 h in preservation solution, lipid peroxide levels of the 0.5 Gy irradiated group were significantly lower than those of the sham irradiated group. Following storage for 24 h in preservation solution, the activity of SOD and catalase of the 1.0 Gy irradiated group were significantly higher than those of the sham irradiated group. Hepatocytes stored in saline solution were vacuolated. However, no vacuole formation was observed in hepatocytes stored in preservation solution. These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts. Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.

No MeSH data available.


Related in: MedlinePlus