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Fungicidal action of hydroxyl radicals generated by ultrasound in water.

Iwasawa A, Saito K, Mokudai T, Kohno M, Ozawa T, Niwano Y - J Clin Biochem Nutr (2009)

Bottom Line: It is well known that hydroxyl radicals are generated by ultrasound in water.This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature.The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

View Article: PubMed Central - PubMed

Affiliation: Tissue Culture Laboratory, Showa University Fujigaoka Hospital, 1-30, Fujigaoka, Aoba-ku, Yokohama, Kanagawa 227-8501, Japan.

ABSTRACT
It is well known that hydroxyl radicals are generated by ultrasound in water. This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature. The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

No MeSH data available.


Related in: MedlinePlus

SEM images of T. mentagrophytes conidia after 5 min in 50°C water exposed (right) or not exposed (left) to ultrasound. Scale bar: 1 µm.
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Figure 6: SEM images of T. mentagrophytes conidia after 5 min in 50°C water exposed (right) or not exposed (left) to ultrasound. Scale bar: 1 µm.

Mentions: A SEM image of T. mentagrophytes conidia exposed for 5 min to ultrasound in water at 50°C (Fig. 6) shows that the ultrasound caused the conidia to collapse and their surfaces to exfoliate. TEM images of T. mentagrophytes conidia exposed to ultrasound radiation at 50°C for different periods of time (Fig. 7) indicate that the exposure degraded the cytoplasmic structure in ways that are likely to lead to necrosis [20–22]. In the references, the term “necrosis” is used to describe irreversible structural degeneration such as crushed, bent, and flattened cells.


Fungicidal action of hydroxyl radicals generated by ultrasound in water.

Iwasawa A, Saito K, Mokudai T, Kohno M, Ozawa T, Niwano Y - J Clin Biochem Nutr (2009)

SEM images of T. mentagrophytes conidia after 5 min in 50°C water exposed (right) or not exposed (left) to ultrasound. Scale bar: 1 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2735635&req=5

Figure 6: SEM images of T. mentagrophytes conidia after 5 min in 50°C water exposed (right) or not exposed (left) to ultrasound. Scale bar: 1 µm.
Mentions: A SEM image of T. mentagrophytes conidia exposed for 5 min to ultrasound in water at 50°C (Fig. 6) shows that the ultrasound caused the conidia to collapse and their surfaces to exfoliate. TEM images of T. mentagrophytes conidia exposed to ultrasound radiation at 50°C for different periods of time (Fig. 7) indicate that the exposure degraded the cytoplasmic structure in ways that are likely to lead to necrosis [20–22]. In the references, the term “necrosis” is used to describe irreversible structural degeneration such as crushed, bent, and flattened cells.

Bottom Line: It is well known that hydroxyl radicals are generated by ultrasound in water.This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature.The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

View Article: PubMed Central - PubMed

Affiliation: Tissue Culture Laboratory, Showa University Fujigaoka Hospital, 1-30, Fujigaoka, Aoba-ku, Yokohama, Kanagawa 227-8501, Japan.

ABSTRACT
It is well known that hydroxyl radicals are generated by ultrasound in water. This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature. The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

No MeSH data available.


Related in: MedlinePlus