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Fungicidal action of hydroxyl radicals generated by ultrasound in water.

Iwasawa A, Saito K, Mokudai T, Kohno M, Ozawa T, Niwano Y - J Clin Biochem Nutr (2009)

Bottom Line: It is well known that hydroxyl radicals are generated by ultrasound in water.This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature.The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

View Article: PubMed Central - PubMed

Affiliation: Tissue Culture Laboratory, Showa University Fujigaoka Hospital, 1-30, Fujigaoka, Aoba-ku, Yokohama, Kanagawa 227-8501, Japan.

ABSTRACT
It is well known that hydroxyl radicals are generated by ultrasound in water. This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature. The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

No MeSH data available.


Related in: MedlinePlus

Representative ESR spectra of DMPO-OH generated by the ultrasound system at 20°C and 40°C.
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Figure 2: Representative ESR spectra of DMPO-OH generated by the ultrasound system at 20°C and 40°C.

Mentions: Fig. 2 shows the representative ESR spectra of DMPO-OH generated by ultrasound in water. The hyperfine coupling constants of the ESR signal were aN = aH = 1.49 mT. The effects of water temperature and exposure time on the amount of ·OH generated when water was exposed to ultrasound are summarized in Figs. 3 and 4, where one sees that the DMPO-OH concentration increased with time and with increasing temperature. The effects of water temperature and exposure time on the fungicidal activity of water exposed to ultrasound are shown in Fig. 5, where one sees that the fungicidal effect of ultrasound radiation was enhanced with time and increasing water temperature and that four of the five strains tested did not survive 10 min in 50°C water exposed to ultrasound. One also sees from the bottom-right bar in Fig. 5 that 50°C without ultrasound had almost no fungicidal effect. Since hydroxyl radical generation increased with ultrasound duration and water temperature, the fungicidal action shown in Fig. 5 is likely to be due to attributable to hydroxyl radicals generated by ultrasound in water.


Fungicidal action of hydroxyl radicals generated by ultrasound in water.

Iwasawa A, Saito K, Mokudai T, Kohno M, Ozawa T, Niwano Y - J Clin Biochem Nutr (2009)

Representative ESR spectra of DMPO-OH generated by the ultrasound system at 20°C and 40°C.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2735635&req=5

Figure 2: Representative ESR spectra of DMPO-OH generated by the ultrasound system at 20°C and 40°C.
Mentions: Fig. 2 shows the representative ESR spectra of DMPO-OH generated by ultrasound in water. The hyperfine coupling constants of the ESR signal were aN = aH = 1.49 mT. The effects of water temperature and exposure time on the amount of ·OH generated when water was exposed to ultrasound are summarized in Figs. 3 and 4, where one sees that the DMPO-OH concentration increased with time and with increasing temperature. The effects of water temperature and exposure time on the fungicidal activity of water exposed to ultrasound are shown in Fig. 5, where one sees that the fungicidal effect of ultrasound radiation was enhanced with time and increasing water temperature and that four of the five strains tested did not survive 10 min in 50°C water exposed to ultrasound. One also sees from the bottom-right bar in Fig. 5 that 50°C without ultrasound had almost no fungicidal effect. Since hydroxyl radical generation increased with ultrasound duration and water temperature, the fungicidal action shown in Fig. 5 is likely to be due to attributable to hydroxyl radicals generated by ultrasound in water.

Bottom Line: It is well known that hydroxyl radicals are generated by ultrasound in water.This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature.The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

View Article: PubMed Central - PubMed

Affiliation: Tissue Culture Laboratory, Showa University Fujigaoka Hospital, 1-30, Fujigaoka, Aoba-ku, Yokohama, Kanagawa 227-8501, Japan.

ABSTRACT
It is well known that hydroxyl radicals are generated by ultrasound in water. This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature. The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

No MeSH data available.


Related in: MedlinePlus