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Effects of Lipid Peroxidation-Derived Products on the Growth of Human Colorectal Cancer Cell Line HT-29.

Sakuma S, Sumi H, Kohda T, Arakawa Y, Fujimoto Y - J Clin Biochem Nutr (2009)

Bottom Line: However, to date the effects of lipid peroxidation-derived products that are formed from fat (especially free or esterified unsaturated fatty acids) on the initiation or progression of colorectal cancer have not been investigated extensively.Therefore, in the present study, we examined the effects of fatty acids, fatty acid hydroperoxides and aldehydes on the growth of human colorectal cancer cell line HT-29.At concentrations of 1 and 10 microM, linoleic, arachidonic and eicosapentaenoic acids, and 13-hydroperoxyoctadecadienoic and 15-hydroperoxyeicosapentaenoic acids had no significant effects on the growth of HT-29 cells. 4-Hydroxynonenal and 4-hydroxyhexenal had no significant effects on the growth of HT-29 cells up to 10 microM, whereas 4-oxononenal potently inhibited HT-29 cell growth (1-10 microM, 16-85% inhibition).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.

ABSTRACT
Epidemiologic investigations indicate a close relationship between colorectal cancer and fat intake. However, to date the effects of lipid peroxidation-derived products that are formed from fat (especially free or esterified unsaturated fatty acids) on the initiation or progression of colorectal cancer have not been investigated extensively. Therefore, in the present study, we examined the effects of fatty acids, fatty acid hydroperoxides and aldehydes on the growth of human colorectal cancer cell line HT-29. At concentrations of 1 and 10 microM, linoleic, arachidonic and eicosapentaenoic acids, and 13-hydroperoxyoctadecadienoic and 15-hydroperoxyeicosapentaenoic acids had no significant effects on the growth of HT-29 cells. 4-Hydroxynonenal and 4-hydroxyhexenal had no significant effects on the growth of HT-29 cells up to 10 microM, whereas 4-oxononenal potently inhibited HT-29 cell growth (1-10 microM, 16-85% inhibition). Further experiments concerning DNA fragmentation, expression levels of Bax and Bcl-2 mRNA, expression levels of pro-caspase-3 and caspase-3 proteins, and activity of caspase-3 suggested that 4-oxononenal may increase the sensitivity of HT-29 cells to apoptosis through a decreased expression level of Bcl-2 and then increased formation of caspase-3 from pro-caspase-3.

No MeSH data available.


Related in: MedlinePlus

Effects of linoleic (LA), arachidonic (AA) and eicosapentaenoic (EPA) acids, and 13-hydroperoxyoctadecadienoic (13-HPODE) and 15-hydroperoxyeicosapentaenoic (15-HPEPE) acids on the growth of HT-29 cells. HT-29 cells were incubated with or without 1 or 10 µM LA, AA, EPA, 13-HPODE and 15-HPEPE for 48 h at 37°C in a humidified atmosphere of 5% CO2. Cell growth was assayed by an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Each bar indicates the mean of 3 experiments; vertical lines show SE. PUFA, polyunsaturated fatty acid.
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Figure 1: Effects of linoleic (LA), arachidonic (AA) and eicosapentaenoic (EPA) acids, and 13-hydroperoxyoctadecadienoic (13-HPODE) and 15-hydroperoxyeicosapentaenoic (15-HPEPE) acids on the growth of HT-29 cells. HT-29 cells were incubated with or without 1 or 10 µM LA, AA, EPA, 13-HPODE and 15-HPEPE for 48 h at 37°C in a humidified atmosphere of 5% CO2. Cell growth was assayed by an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Each bar indicates the mean of 3 experiments; vertical lines show SE. PUFA, polyunsaturated fatty acid.

Mentions: Fig. 1 shows the results of cell viability after 48 h treatment with n-6 (LA and AA) and n-3 (EPA) PUFAs, and hydroperoxy adducts of LA (13-HPODE) and EPA (15-HPEPE). These fatty acids and hydroperoxides had no significant effect on the growth of HT-29 cells at concentrations of 1 and 10 µM. On the other hand, Fig. 2 illustrates the efficacies of unsaturated aldehydes generated from 13-HPODE (4-HNE and 4-ONE) and from 15-HPEPE (4-HHE) [6] on the growth of HT-29 cells. No significant alteration was observed with 4-HNE and 4-HHE at concentrations ranging from 1 to 10 µM, whereas 4-ONE potently reduced HT-29 cell growth at concentrations of 5 and 10 µM (59 and 85% inhibition).


Effects of Lipid Peroxidation-Derived Products on the Growth of Human Colorectal Cancer Cell Line HT-29.

Sakuma S, Sumi H, Kohda T, Arakawa Y, Fujimoto Y - J Clin Biochem Nutr (2009)

Effects of linoleic (LA), arachidonic (AA) and eicosapentaenoic (EPA) acids, and 13-hydroperoxyoctadecadienoic (13-HPODE) and 15-hydroperoxyeicosapentaenoic (15-HPEPE) acids on the growth of HT-29 cells. HT-29 cells were incubated with or without 1 or 10 µM LA, AA, EPA, 13-HPODE and 15-HPEPE for 48 h at 37°C in a humidified atmosphere of 5% CO2. Cell growth was assayed by an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Each bar indicates the mean of 3 experiments; vertical lines show SE. PUFA, polyunsaturated fatty acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2735629&req=5

Figure 1: Effects of linoleic (LA), arachidonic (AA) and eicosapentaenoic (EPA) acids, and 13-hydroperoxyoctadecadienoic (13-HPODE) and 15-hydroperoxyeicosapentaenoic (15-HPEPE) acids on the growth of HT-29 cells. HT-29 cells were incubated with or without 1 or 10 µM LA, AA, EPA, 13-HPODE and 15-HPEPE for 48 h at 37°C in a humidified atmosphere of 5% CO2. Cell growth was assayed by an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Each bar indicates the mean of 3 experiments; vertical lines show SE. PUFA, polyunsaturated fatty acid.
Mentions: Fig. 1 shows the results of cell viability after 48 h treatment with n-6 (LA and AA) and n-3 (EPA) PUFAs, and hydroperoxy adducts of LA (13-HPODE) and EPA (15-HPEPE). These fatty acids and hydroperoxides had no significant effect on the growth of HT-29 cells at concentrations of 1 and 10 µM. On the other hand, Fig. 2 illustrates the efficacies of unsaturated aldehydes generated from 13-HPODE (4-HNE and 4-ONE) and from 15-HPEPE (4-HHE) [6] on the growth of HT-29 cells. No significant alteration was observed with 4-HNE and 4-HHE at concentrations ranging from 1 to 10 µM, whereas 4-ONE potently reduced HT-29 cell growth at concentrations of 5 and 10 µM (59 and 85% inhibition).

Bottom Line: However, to date the effects of lipid peroxidation-derived products that are formed from fat (especially free or esterified unsaturated fatty acids) on the initiation or progression of colorectal cancer have not been investigated extensively.Therefore, in the present study, we examined the effects of fatty acids, fatty acid hydroperoxides and aldehydes on the growth of human colorectal cancer cell line HT-29.At concentrations of 1 and 10 microM, linoleic, arachidonic and eicosapentaenoic acids, and 13-hydroperoxyoctadecadienoic and 15-hydroperoxyeicosapentaenoic acids had no significant effects on the growth of HT-29 cells. 4-Hydroxynonenal and 4-hydroxyhexenal had no significant effects on the growth of HT-29 cells up to 10 microM, whereas 4-oxononenal potently inhibited HT-29 cell growth (1-10 microM, 16-85% inhibition).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.

ABSTRACT
Epidemiologic investigations indicate a close relationship between colorectal cancer and fat intake. However, to date the effects of lipid peroxidation-derived products that are formed from fat (especially free or esterified unsaturated fatty acids) on the initiation or progression of colorectal cancer have not been investigated extensively. Therefore, in the present study, we examined the effects of fatty acids, fatty acid hydroperoxides and aldehydes on the growth of human colorectal cancer cell line HT-29. At concentrations of 1 and 10 microM, linoleic, arachidonic and eicosapentaenoic acids, and 13-hydroperoxyoctadecadienoic and 15-hydroperoxyeicosapentaenoic acids had no significant effects on the growth of HT-29 cells. 4-Hydroxynonenal and 4-hydroxyhexenal had no significant effects on the growth of HT-29 cells up to 10 microM, whereas 4-oxononenal potently inhibited HT-29 cell growth (1-10 microM, 16-85% inhibition). Further experiments concerning DNA fragmentation, expression levels of Bax and Bcl-2 mRNA, expression levels of pro-caspase-3 and caspase-3 proteins, and activity of caspase-3 suggested that 4-oxononenal may increase the sensitivity of HT-29 cells to apoptosis through a decreased expression level of Bcl-2 and then increased formation of caspase-3 from pro-caspase-3.

No MeSH data available.


Related in: MedlinePlus