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Expression of naive/memory (CD45RA/CD45RO) markers by peripheral blood CD4+ and CD8 + T cells in children with asthma.

Machura E, Mazur B, Pieniazek W, Karczewska K - Arch. Immunol. Ther. Exp. (Warsz.) (2008)

Bottom Line: Peripheral blood mononuclear cells were isolated from children with allergic asthma and healthy children.T cell subsets were analyzed by flow cytometry for the expressions of CD45RA, CD45RO, and CD25.There were some differences in the memory compartment of peripheral blood T cells between asthmatic children and healthy controls.

View Article: PubMed Central - PubMed

Affiliation: Chair and Department of Pediatric Gastroenterology, Allergology, and Developmental Disorders, Medical University of Silesia, Zabrze, Poland.

ABSTRACT

Introduction: The role of CD4+ T cells in the immunopathogenesis of asthma is well documented. Little is known about the role of CD8+ T cells. The aim of this study was to assess peripheral blood subsets of CD4+ and CD8+ T cells expressing naive/memory markers (CD45RA+/RO+) and the activation marker (CD25+) in children with allergic asthma.

Materials and methods: Peripheral blood mononuclear cells were isolated from children with allergic asthma and healthy children. T cell subsets were analyzed by flow cytometry for the expressions of CD45RA, CD45RO, and CD25. In this study, some differences in the memory compartment of peripheral blood T cells between asthmatic children and healthy controls were detected.

Results: The absolute number of CD8+ T cells expressing CD45RO was significantly elevated and the percentages of CD3+ T cells expressing activation marker CD25 and of CD4+ T cells expressing memory marker CD45RO were significantly lower in children with asthma compared with controls. No correlation was found between severity of asthma and peripheral blood lymphocyte subsets.

Conclusions: There were some differences in the memory compartment of peripheral blood T cells between asthmatic children and healthy controls. The increase in the number of CD8+ T cells expressing the memory marker (CD45RO) in children with allergic asthma may indicate that CD8+ T cells play a role in the pathogenesis of asthma.

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Related in: MedlinePlus

Percentages and absolute numbers of the CD4+ and CD8+ peripheral blood T-memory marker CD45RO in the study groups. The percentage of CD4+CD45RO+ T cells in children with asthma was significantly lower than in healthy children (p<0.0002), but the absolute numbers did not differ. The percentage of CD8+CD45RO+ T cells tended to be higher (p=0.1) and the number was significantly elevated in children with asthma compared with healthy children (p<0.025). The Mann-Whitney U-test was used to determine significant differences between the two groups.
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Fig1: Percentages and absolute numbers of the CD4+ and CD8+ peripheral blood T-memory marker CD45RO in the study groups. The percentage of CD4+CD45RO+ T cells in children with asthma was significantly lower than in healthy children (p<0.0002), but the absolute numbers did not differ. The percentage of CD8+CD45RO+ T cells tended to be higher (p=0.1) and the number was significantly elevated in children with asthma compared with healthy children (p<0.025). The Mann-Whitney U-test was used to determine significant differences between the two groups.

Mentions: The mean percentages, counts, and SE for all the lymphocyte populations are shown in Table 2. Flow-cytometric analysis revealed no statistically significant differences in the percentages of CD3+, CD4+, CD8+ CD45RA+, CD4+CD45RA+, CD8+CD45RA+, CD45RO+, and CD8+CD45RO+ between the asthma group and healthy controls. The percentage of CD4+CD45RO+ was significantly lower in the asthmatic children (p<0.0002), but the absolute count did not differ between the groups. The CD8+CD45RO+ T cell count was significantly higher in the asthma group (p<0.025) than in the healthy children (Fig. 1).


Expression of naive/memory (CD45RA/CD45RO) markers by peripheral blood CD4+ and CD8 + T cells in children with asthma.

Machura E, Mazur B, Pieniazek W, Karczewska K - Arch. Immunol. Ther. Exp. (Warsz.) (2008)

Percentages and absolute numbers of the CD4+ and CD8+ peripheral blood T-memory marker CD45RO in the study groups. The percentage of CD4+CD45RO+ T cells in children with asthma was significantly lower than in healthy children (p<0.0002), but the absolute numbers did not differ. The percentage of CD8+CD45RO+ T cells tended to be higher (p=0.1) and the number was significantly elevated in children with asthma compared with healthy children (p<0.025). The Mann-Whitney U-test was used to determine significant differences between the two groups.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2734248&req=5

Fig1: Percentages and absolute numbers of the CD4+ and CD8+ peripheral blood T-memory marker CD45RO in the study groups. The percentage of CD4+CD45RO+ T cells in children with asthma was significantly lower than in healthy children (p<0.0002), but the absolute numbers did not differ. The percentage of CD8+CD45RO+ T cells tended to be higher (p=0.1) and the number was significantly elevated in children with asthma compared with healthy children (p<0.025). The Mann-Whitney U-test was used to determine significant differences between the two groups.
Mentions: The mean percentages, counts, and SE for all the lymphocyte populations are shown in Table 2. Flow-cytometric analysis revealed no statistically significant differences in the percentages of CD3+, CD4+, CD8+ CD45RA+, CD4+CD45RA+, CD8+CD45RA+, CD45RO+, and CD8+CD45RO+ between the asthma group and healthy controls. The percentage of CD4+CD45RO+ was significantly lower in the asthmatic children (p<0.0002), but the absolute count did not differ between the groups. The CD8+CD45RO+ T cell count was significantly higher in the asthma group (p<0.025) than in the healthy children (Fig. 1).

Bottom Line: Peripheral blood mononuclear cells were isolated from children with allergic asthma and healthy children.T cell subsets were analyzed by flow cytometry for the expressions of CD45RA, CD45RO, and CD25.There were some differences in the memory compartment of peripheral blood T cells between asthmatic children and healthy controls.

View Article: PubMed Central - PubMed

Affiliation: Chair and Department of Pediatric Gastroenterology, Allergology, and Developmental Disorders, Medical University of Silesia, Zabrze, Poland.

ABSTRACT

Introduction: The role of CD4+ T cells in the immunopathogenesis of asthma is well documented. Little is known about the role of CD8+ T cells. The aim of this study was to assess peripheral blood subsets of CD4+ and CD8+ T cells expressing naive/memory markers (CD45RA+/RO+) and the activation marker (CD25+) in children with allergic asthma.

Materials and methods: Peripheral blood mononuclear cells were isolated from children with allergic asthma and healthy children. T cell subsets were analyzed by flow cytometry for the expressions of CD45RA, CD45RO, and CD25. In this study, some differences in the memory compartment of peripheral blood T cells between asthmatic children and healthy controls were detected.

Results: The absolute number of CD8+ T cells expressing CD45RO was significantly elevated and the percentages of CD3+ T cells expressing activation marker CD25 and of CD4+ T cells expressing memory marker CD45RO were significantly lower in children with asthma compared with controls. No correlation was found between severity of asthma and peripheral blood lymphocyte subsets.

Conclusions: There were some differences in the memory compartment of peripheral blood T cells between asthmatic children and healthy controls. The increase in the number of CD8+ T cells expressing the memory marker (CD45RO) in children with allergic asthma may indicate that CD8+ T cells play a role in the pathogenesis of asthma.

Show MeSH
Related in: MedlinePlus