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Identification of genes responsive to solar simulated UV radiation in human monocyte-derived dendritic cells.

de la Fuente H, Lamana A, Mittelbrunn M, Perez-Gala S, Gonzalez S, García-Diez A, Vega M, Sanchez-Madrid F - PLoS ONE (2009)

Bottom Line: Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system.Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7.These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC-mediated immune responses.

View Article: PubMed Central - PubMed

Affiliation: Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Madrid, Spain.

ABSTRACT
Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC-mediated immune responses.

Show MeSH
Summary of Genes modified on DCs by Solar-Simulated irradiation.Genes down-regulated or up-regulated in UV irradiated human DCs, compared to non-irradiated DCs were grouped by functional groups, using Gene Ontology designations (WebGestalt) and published literature. Genes bold and italic correspond to down-regulated-genes. (*) Genes selected to corroborate by PCR.
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pone-0006735-g004: Summary of Genes modified on DCs by Solar-Simulated irradiation.Genes down-regulated or up-regulated in UV irradiated human DCs, compared to non-irradiated DCs were grouped by functional groups, using Gene Ontology designations (WebGestalt) and published literature. Genes bold and italic correspond to down-regulated-genes. (*) Genes selected to corroborate by PCR.

Mentions: The classification of genes according of their cellular function, following an extensive review of the literature, facilitates the understanding of cellular processes affected in DCs by UV radiation. Fig. 4 shows a summary of the genes modulated by UV solar simulated irradiation clustered by functional groups. In all functional groups showed, the major percentage of genes was up-regulated. However, other functional groups including DNA and RNA Metabolism and Immune System Process mainly had down-regulated genes.


Identification of genes responsive to solar simulated UV radiation in human monocyte-derived dendritic cells.

de la Fuente H, Lamana A, Mittelbrunn M, Perez-Gala S, Gonzalez S, García-Diez A, Vega M, Sanchez-Madrid F - PLoS ONE (2009)

Summary of Genes modified on DCs by Solar-Simulated irradiation.Genes down-regulated or up-regulated in UV irradiated human DCs, compared to non-irradiated DCs were grouped by functional groups, using Gene Ontology designations (WebGestalt) and published literature. Genes bold and italic correspond to down-regulated-genes. (*) Genes selected to corroborate by PCR.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2727914&req=5

pone-0006735-g004: Summary of Genes modified on DCs by Solar-Simulated irradiation.Genes down-regulated or up-regulated in UV irradiated human DCs, compared to non-irradiated DCs were grouped by functional groups, using Gene Ontology designations (WebGestalt) and published literature. Genes bold and italic correspond to down-regulated-genes. (*) Genes selected to corroborate by PCR.
Mentions: The classification of genes according of their cellular function, following an extensive review of the literature, facilitates the understanding of cellular processes affected in DCs by UV radiation. Fig. 4 shows a summary of the genes modulated by UV solar simulated irradiation clustered by functional groups. In all functional groups showed, the major percentage of genes was up-regulated. However, other functional groups including DNA and RNA Metabolism and Immune System Process mainly had down-regulated genes.

Bottom Line: Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system.Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7.These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC-mediated immune responses.

View Article: PubMed Central - PubMed

Affiliation: Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Madrid, Spain.

ABSTRACT
Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC-mediated immune responses.

Show MeSH