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Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity.

Witzel K, Weidner A, Surabhi GK, Börner A, Mock HP - J. Exp. Bot. (2009)

Bottom Line: Multivariate analysis of the resulting protein patterns revealed cultivar-specific and salt stress-responsive protein expression.Hierarchical clustering was applied to detect similar protein expression patterns.This study emphasizes the role of proteins involved in ROS detoxification during salinity stress, and identified potential candidates for increasing salt tolerance in barley.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.

ABSTRACT
In addition to drought and extreme temperatures, soil salinity represents a growing threat to crop productivity. Among the cereal crops, barley is considered as notably salt tolerant, and cultivars show considerable variation for tolerance towards salinity stress. In order to unravel the molecular mechanisms underlying salt stress tolerance and to utilize the natural genetic variation of barley accessions, a series of hydroponics-based salinity stress experiments was conducted using two genetic mapping parents, cvs Steptoe and Morex, which display contrasting levels of salinity tolerance. The proteome of roots from both genotypes was investigated as displayed by two-dimensional gel electrophoresis, and comparisons were made between plants grown under non-saline and saline conditions. Multivariate analysis of the resulting protein patterns revealed cultivar-specific and salt stress-responsive protein expression. Mass spectrometry-based identification was successful for 26 out of 39 selected protein spots. Hierarchical clustering was applied to detect similar protein expression patterns. Among those, two proteins involved in the glutathione-based detoxification of reactive oxygen species (ROS) were more abundant in the tolerant genotype, while proteins involved in iron uptake were expressed at a higher level in the sensitive one. This study emphasizes the role of proteins involved in ROS detoxification during salinity stress, and identified potential candidates for increasing salt tolerance in barley.

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Related in: MedlinePlus

Assessment of technical and biological variation in protein expression profiles of control and treated samples from cvs Steptoe and Morex. Used for calculation were differentially regulated spots with P <0.05. Principle component (PC) 1 revealed genotype-specific expression and accounted for 38.6% of the variation, while PC 2 showed treatment-responsive expression and accounted for 20.9% of the variation.
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fig2: Assessment of technical and biological variation in protein expression profiles of control and treated samples from cvs Steptoe and Morex. Used for calculation were differentially regulated spots with P <0.05. Principle component (PC) 1 revealed genotype-specific expression and accounted for 38.6% of the variation, while PC 2 showed treatment-responsive expression and accounted for 20.9% of the variation.

Mentions: 2-D gels from three biological experiments were analysed to detect proteins that were reproducibly regulated in the same manner either cultivar specifically or in response to the stress treatment. Approximately 1100 protein spots were detected on the 2-D gels, of which ∼760 were matched between groups of technical replicates. PCA indicated a grouping of technical replicates and a distinct separation of genotypes as well as treatments (Fig. 2). The contrast between salinity-stressed and non-stressed samples identified salinity-induced alterations in the protein profile. Based on the greater difference between control and treated profiles in cv. Steptoe as compared with cv. Morex, it is concluded that more proteins are affected by stress-specific regulation in the less tolerant genotype.


Salt stress-induced alterations in the root proteome of barley genotypes with contrasting response towards salinity.

Witzel K, Weidner A, Surabhi GK, Börner A, Mock HP - J. Exp. Bot. (2009)

Assessment of technical and biological variation in protein expression profiles of control and treated samples from cvs Steptoe and Morex. Used for calculation were differentially regulated spots with P <0.05. Principle component (PC) 1 revealed genotype-specific expression and accounted for 38.6% of the variation, while PC 2 showed treatment-responsive expression and accounted for 20.9% of the variation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2724703&req=5

fig2: Assessment of technical and biological variation in protein expression profiles of control and treated samples from cvs Steptoe and Morex. Used for calculation were differentially regulated spots with P <0.05. Principle component (PC) 1 revealed genotype-specific expression and accounted for 38.6% of the variation, while PC 2 showed treatment-responsive expression and accounted for 20.9% of the variation.
Mentions: 2-D gels from three biological experiments were analysed to detect proteins that were reproducibly regulated in the same manner either cultivar specifically or in response to the stress treatment. Approximately 1100 protein spots were detected on the 2-D gels, of which ∼760 were matched between groups of technical replicates. PCA indicated a grouping of technical replicates and a distinct separation of genotypes as well as treatments (Fig. 2). The contrast between salinity-stressed and non-stressed samples identified salinity-induced alterations in the protein profile. Based on the greater difference between control and treated profiles in cv. Steptoe as compared with cv. Morex, it is concluded that more proteins are affected by stress-specific regulation in the less tolerant genotype.

Bottom Line: Multivariate analysis of the resulting protein patterns revealed cultivar-specific and salt stress-responsive protein expression.Hierarchical clustering was applied to detect similar protein expression patterns.This study emphasizes the role of proteins involved in ROS detoxification during salinity stress, and identified potential candidates for increasing salt tolerance in barley.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.

ABSTRACT
In addition to drought and extreme temperatures, soil salinity represents a growing threat to crop productivity. Among the cereal crops, barley is considered as notably salt tolerant, and cultivars show considerable variation for tolerance towards salinity stress. In order to unravel the molecular mechanisms underlying salt stress tolerance and to utilize the natural genetic variation of barley accessions, a series of hydroponics-based salinity stress experiments was conducted using two genetic mapping parents, cvs Steptoe and Morex, which display contrasting levels of salinity tolerance. The proteome of roots from both genotypes was investigated as displayed by two-dimensional gel electrophoresis, and comparisons were made between plants grown under non-saline and saline conditions. Multivariate analysis of the resulting protein patterns revealed cultivar-specific and salt stress-responsive protein expression. Mass spectrometry-based identification was successful for 26 out of 39 selected protein spots. Hierarchical clustering was applied to detect similar protein expression patterns. Among those, two proteins involved in the glutathione-based detoxification of reactive oxygen species (ROS) were more abundant in the tolerant genotype, while proteins involved in iron uptake were expressed at a higher level in the sensitive one. This study emphasizes the role of proteins involved in ROS detoxification during salinity stress, and identified potential candidates for increasing salt tolerance in barley.

Show MeSH
Related in: MedlinePlus