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Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage.

Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, von Korff M, Varshney RK, Graner A, Valkoun J - J. Exp. Bot. (2009)

Bottom Line: Moreover, 17 genes were abundantly expressed in Martin and HS41-1 compared with Moroc9-75 under both drought and control conditions.Among them, seven known annotated genes might enhance drought tolerance through signalling [such as calcium-dependent protein kinase (CDPK) and membrane steroid binding protein (MSBP)], anti-senescence (G2 pea dark accumulated protein, GDA2), and detoxification (glutathione S-transferase, GST) pathways.These results could provide new insights into further understanding of drought-tolerance mechanisms in barley.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Guangzhou University, Guangzhou 510006, China.

ABSTRACT
Drought tolerance is a key trait for increasing and stabilizing barley productivity in dry areas worldwide. Identification of the genes responsible for drought tolerance in barley (Hordeum vulgare L.) will facilitate understanding of the molecular mechanisms of drought tolerance, and also facilitate the genetic improvement of barley through marker-assisted selection or gene transformation. To monitor the changes in gene expression at the transcriptional level in barley leaves during the reproductive stage under drought conditions, the 22K Affymetrix Barley 1 microarray was used to screen two drought-tolerant barley genotypes, Martin and Hordeum spontaneum 41-1 (HS41-1), and one drought-sensitive genotype Moroc9-75. Seventeen genes were expressed exclusively in the two drought-tolerant genotypes under drought stress, and their encoded proteins may play significant roles in enhancing drought tolerance through controlling stomatal closure via carbon metabolism (NADP malic enzyme, NADP-ME, and pyruvate dehydrogenase, PDH), synthesizing the osmoprotectant glycine-betaine (C-4 sterol methyl oxidase, CSMO), generating protectants against reactive-oxygen-species scavenging (aldehyde dehydrogenase,ALDH, ascorbate-dependent oxidoreductase, ADOR), and stabilizing membranes and proteins (heat-shock protein 17.8, HSP17.8, and dehydrin 3, DHN3). Moreover, 17 genes were abundantly expressed in Martin and HS41-1 compared with Moroc9-75 under both drought and control conditions. These genes were possibly constitutively expressed in drought-tolerant genotypes. Among them, seven known annotated genes might enhance drought tolerance through signalling [such as calcium-dependent protein kinase (CDPK) and membrane steroid binding protein (MSBP)], anti-senescence (G2 pea dark accumulated protein, GDA2), and detoxification (glutathione S-transferase, GST) pathways. In addition, 18 genes, including those encoding Delta(l)-pyrroline-5-carboxylate synthetase (P5CS), protein phosphatase 2C-like protein (PP2C), and several chaperones, were differentially expressed in all genotypes under drought; thus they were more likely to be general drought-responsive genes in barley. These results could provide new insights into further understanding of drought-tolerance mechanisms in barley.

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Related in: MedlinePlus

Chlorophyll contents and maximum quantum yield of PSII (Fv/Fm) of three genotypes under well-watered conditions (70% available water in the soil) and drought stress (10% available water in the soil). Results are presented as mean ±SD of six individual measurements. (A) and (B) represent relative chlorophyll content and ratio of Fv/Fm, respectively, for three genotypes (Martin, HS41-1, and Moroc9-75).
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fig1: Chlorophyll contents and maximum quantum yield of PSII (Fv/Fm) of three genotypes under well-watered conditions (70% available water in the soil) and drought stress (10% available water in the soil). Results are presented as mean ±SD of six individual measurements. (A) and (B) represent relative chlorophyll content and ratio of Fv/Fm, respectively, for three genotypes (Martin, HS41-1, and Moroc9-75).

Mentions: Although chlorophyll content decreased in all three genotypes under post-anthesis drought conditions (Fig. 1a), there were differential responses between drought-tolerant and drought-sensitive genotypes. A visible decline in chlorophyll content started 3 d after drought stress in the drought-sensitive Moroc9-75 and at 5 d in drought-tolerant Martin and HS41-1. After 13 d of drought stress, the relative reduction in chlorophyll content was at least double in Moroc9-75 (41.8%) compared with Martin (24.9%) and HS41-1 (19.4%).


Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage.

Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, von Korff M, Varshney RK, Graner A, Valkoun J - J. Exp. Bot. (2009)

Chlorophyll contents and maximum quantum yield of PSII (Fv/Fm) of three genotypes under well-watered conditions (70% available water in the soil) and drought stress (10% available water in the soil). Results are presented as mean ±SD of six individual measurements. (A) and (B) represent relative chlorophyll content and ratio of Fv/Fm, respectively, for three genotypes (Martin, HS41-1, and Moroc9-75).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2724701&req=5

fig1: Chlorophyll contents and maximum quantum yield of PSII (Fv/Fm) of three genotypes under well-watered conditions (70% available water in the soil) and drought stress (10% available water in the soil). Results are presented as mean ±SD of six individual measurements. (A) and (B) represent relative chlorophyll content and ratio of Fv/Fm, respectively, for three genotypes (Martin, HS41-1, and Moroc9-75).
Mentions: Although chlorophyll content decreased in all three genotypes under post-anthesis drought conditions (Fig. 1a), there were differential responses between drought-tolerant and drought-sensitive genotypes. A visible decline in chlorophyll content started 3 d after drought stress in the drought-sensitive Moroc9-75 and at 5 d in drought-tolerant Martin and HS41-1. After 13 d of drought stress, the relative reduction in chlorophyll content was at least double in Moroc9-75 (41.8%) compared with Martin (24.9%) and HS41-1 (19.4%).

Bottom Line: Moreover, 17 genes were abundantly expressed in Martin and HS41-1 compared with Moroc9-75 under both drought and control conditions.Among them, seven known annotated genes might enhance drought tolerance through signalling [such as calcium-dependent protein kinase (CDPK) and membrane steroid binding protein (MSBP)], anti-senescence (G2 pea dark accumulated protein, GDA2), and detoxification (glutathione S-transferase, GST) pathways.These results could provide new insights into further understanding of drought-tolerance mechanisms in barley.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Guangzhou University, Guangzhou 510006, China.

ABSTRACT
Drought tolerance is a key trait for increasing and stabilizing barley productivity in dry areas worldwide. Identification of the genes responsible for drought tolerance in barley (Hordeum vulgare L.) will facilitate understanding of the molecular mechanisms of drought tolerance, and also facilitate the genetic improvement of barley through marker-assisted selection or gene transformation. To monitor the changes in gene expression at the transcriptional level in barley leaves during the reproductive stage under drought conditions, the 22K Affymetrix Barley 1 microarray was used to screen two drought-tolerant barley genotypes, Martin and Hordeum spontaneum 41-1 (HS41-1), and one drought-sensitive genotype Moroc9-75. Seventeen genes were expressed exclusively in the two drought-tolerant genotypes under drought stress, and their encoded proteins may play significant roles in enhancing drought tolerance through controlling stomatal closure via carbon metabolism (NADP malic enzyme, NADP-ME, and pyruvate dehydrogenase, PDH), synthesizing the osmoprotectant glycine-betaine (C-4 sterol methyl oxidase, CSMO), generating protectants against reactive-oxygen-species scavenging (aldehyde dehydrogenase,ALDH, ascorbate-dependent oxidoreductase, ADOR), and stabilizing membranes and proteins (heat-shock protein 17.8, HSP17.8, and dehydrin 3, DHN3). Moreover, 17 genes were abundantly expressed in Martin and HS41-1 compared with Moroc9-75 under both drought and control conditions. These genes were possibly constitutively expressed in drought-tolerant genotypes. Among them, seven known annotated genes might enhance drought tolerance through signalling [such as calcium-dependent protein kinase (CDPK) and membrane steroid binding protein (MSBP)], anti-senescence (G2 pea dark accumulated protein, GDA2), and detoxification (glutathione S-transferase, GST) pathways. In addition, 18 genes, including those encoding Delta(l)-pyrroline-5-carboxylate synthetase (P5CS), protein phosphatase 2C-like protein (PP2C), and several chaperones, were differentially expressed in all genotypes under drought; thus they were more likely to be general drought-responsive genes in barley. These results could provide new insights into further understanding of drought-tolerance mechanisms in barley.

Show MeSH
Related in: MedlinePlus