Limits...
Lipid and protein accumulation in developing seeds of three lupine species: Lupinus luteus L., Lupinus albus L., and Lupinus mutabilis Sweet.

Borek S, Pukacka S, Michalski K, Ratajczak L - J. Exp. Bot. (2009)

Bottom Line: Asparagine caused an increase in protein accumulation and simultaneously decreased the lipid content, but nitrate increased accumulation of both protein and lipid.The main fatty acid in yellow lupine cotyledons was linoleic acid, in white lupine it was oleic acid, and in Andean lupine it was both linoleic and oleic acids.The relationship between stimulation of lipid and protein accumulation by nitrate in developing lupine cotyledons and enhanced carbon flux through glycolysis caused by the inorganic nitrogen form is discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland. borek@amu.edu.pl

ABSTRACT
A comparative study was carried out on the dynamics of lipid accumulation in developing seeds of three lupine species. Lupine seeds differ in lipid content; yellow lupine (Lupinus luteus L.) seeds contain about 6%, white lupine (Lupinus albus L.) 7-14%, and Andean lupine (Lupinus mutabilis Sweet) about 20% of lipids by dry mass. Cotyledons from developing seeds were isolated and cultured in vitro for 96 h on Heller medium with 60 mM sucrose (+S) or without sucrose (-S). Each medium was additionally enriched with 35 mM asparagine or 35 mM NaNO3. Asparagine caused an increase in protein accumulation and simultaneously decreased the lipid content, but nitrate increased accumulation of both protein and lipid. Experiments with [1-14C]acetate and [2-14C]acetate showed that the decrease in lipid accumulation in developing lupine seeds resulted from exhaustion of lipid precursors rather than from degradation or modification of the enzymatic apparatus. The carbon atom from the C-1 position of acetate was liberated mainly as CO2, whereas the carbon atom from the C-2 position was preferentially used in anabolic pathways. The dominant phospholipid in the investigated lupine seed storage organs was phosphatidylcholine. The main fatty acid in yellow lupine cotyledons was linoleic acid, in white lupine it was oleic acid, and in Andean lupine it was both linoleic and oleic acids. The relationship between stimulation of lipid and protein accumulation by nitrate in developing lupine cotyledons and enhanced carbon flux through glycolysis caused by the inorganic nitrogen form is discussed.

Show MeSH

Related in: MedlinePlus

Total lipid level in whole seeds from five developmental stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC2724698&req=5

fig1: Total lipid level in whole seeds from five developmental stages.

Mentions: The total lipid level in the five developmental stages was determined. In the three lupine species the lipid level increased almost linearly during the seed developmental period and reached the maximal level in stage V. In that stage, the highest content of lipid was in Andean lupine seeds, whereas the lowest was noted in the seeds of yellow lupine (Fig. 1). For the following experiments, cotyledons from developmental stage III were used, because in that stage the lipid level was 45–50% of the maximum content. In consecutive stages the soluble protein content was also increased, but the highest protein accumulation took place in stages IV and V (Fig. 2). In these developmental stages, in contrast to the lipid level, the highest amount of protein was observed in the seeds of yellow lupine.


Lipid and protein accumulation in developing seeds of three lupine species: Lupinus luteus L., Lupinus albus L., and Lupinus mutabilis Sweet.

Borek S, Pukacka S, Michalski K, Ratajczak L - J. Exp. Bot. (2009)

Total lipid level in whole seeds from five developmental stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2724698&req=5

fig1: Total lipid level in whole seeds from five developmental stages.
Mentions: The total lipid level in the five developmental stages was determined. In the three lupine species the lipid level increased almost linearly during the seed developmental period and reached the maximal level in stage V. In that stage, the highest content of lipid was in Andean lupine seeds, whereas the lowest was noted in the seeds of yellow lupine (Fig. 1). For the following experiments, cotyledons from developmental stage III were used, because in that stage the lipid level was 45–50% of the maximum content. In consecutive stages the soluble protein content was also increased, but the highest protein accumulation took place in stages IV and V (Fig. 2). In these developmental stages, in contrast to the lipid level, the highest amount of protein was observed in the seeds of yellow lupine.

Bottom Line: Asparagine caused an increase in protein accumulation and simultaneously decreased the lipid content, but nitrate increased accumulation of both protein and lipid.The main fatty acid in yellow lupine cotyledons was linoleic acid, in white lupine it was oleic acid, and in Andean lupine it was both linoleic and oleic acids.The relationship between stimulation of lipid and protein accumulation by nitrate in developing lupine cotyledons and enhanced carbon flux through glycolysis caused by the inorganic nitrogen form is discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland. borek@amu.edu.pl

ABSTRACT
A comparative study was carried out on the dynamics of lipid accumulation in developing seeds of three lupine species. Lupine seeds differ in lipid content; yellow lupine (Lupinus luteus L.) seeds contain about 6%, white lupine (Lupinus albus L.) 7-14%, and Andean lupine (Lupinus mutabilis Sweet) about 20% of lipids by dry mass. Cotyledons from developing seeds were isolated and cultured in vitro for 96 h on Heller medium with 60 mM sucrose (+S) or without sucrose (-S). Each medium was additionally enriched with 35 mM asparagine or 35 mM NaNO3. Asparagine caused an increase in protein accumulation and simultaneously decreased the lipid content, but nitrate increased accumulation of both protein and lipid. Experiments with [1-14C]acetate and [2-14C]acetate showed that the decrease in lipid accumulation in developing lupine seeds resulted from exhaustion of lipid precursors rather than from degradation or modification of the enzymatic apparatus. The carbon atom from the C-1 position of acetate was liberated mainly as CO2, whereas the carbon atom from the C-2 position was preferentially used in anabolic pathways. The dominant phospholipid in the investigated lupine seed storage organs was phosphatidylcholine. The main fatty acid in yellow lupine cotyledons was linoleic acid, in white lupine it was oleic acid, and in Andean lupine it was both linoleic and oleic acids. The relationship between stimulation of lipid and protein accumulation by nitrate in developing lupine cotyledons and enhanced carbon flux through glycolysis caused by the inorganic nitrogen form is discussed.

Show MeSH
Related in: MedlinePlus