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Developmental expression profile of the YY2 gene in mice.

Drews D, Klar M, Dame C, Bräuer AU - BMC Dev. Biol. (2009)

Bottom Line: Again, only yy2 showed significantly altered gene expression levels among the cell types.For the first time, developmental changes of yy2 transcription became obvious in various areas of the brain.This suggests that yy2 is involved in developmental gene regulation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neonatology, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. david.drews@charite.de

ABSTRACT

Background: The transcription factor Yin Yang 2 (YY2) shares a structural and functional highly homologue DNA-binding domain with the ubiquitously expressed YY1 protein, which has been implicated in regulating fundamental biological processes. However, the biological relevance of YY2 has not been identified yet.

Results: Towards the understanding of YY2 biology, we analyzed in detail the expression pattern of yy2 in various organs during embryonic and postnatal mouse development till adulthood. Thereby, a constant yy2 level was detected in heart and lung tissue, whereas in different brain regions yy2 expression was dynamically regulated. Interestingly, in any analyzed tissue neither the homologue yy1 nor the mbtps2 gene showed changes in mRNA expression levels like yy2, although the intronless yy2 gene is located within the mbtps2 locus.Furthermore, we detected yy1, yy2, and mbtps2 mRNA in primary mouse neurons, microglia cells, and astrocytes. In comparison to yy2 and mbtps2, yy1 revealed the highest expression level in all cell types. Again, only yy2 showed significantly altered gene expression levels among the cell types. Higher yy2 expression levels were detected in microglia cells and astrocytes than in primary neurons.

Conclusion: Yy2 expression in the heart and lung is constitutively expressed during embryogenesis and in adult mice. For the first time, developmental changes of yy2 transcription became obvious in various areas of the brain. This suggests that yy2 is involved in developmental gene regulation.

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Quantification of yy2, mbtps2 and yy1 in developing neocortex. Quantitative expression analyses (TaqMan real-time PCR) of yy2, mbtps2 and yy1 transcripts in the neocortex during development and in adult mice (E14 to P60). The expression of yy2 reaches a minimum around day P5. Expression levels are normalized to β-actin. (n = 3 sets; * p < 0.05; ** p < 0.01; statistical significances refer to E14).
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Figure 5: Quantification of yy2, mbtps2 and yy1 in developing neocortex. Quantitative expression analyses (TaqMan real-time PCR) of yy2, mbtps2 and yy1 transcripts in the neocortex during development and in adult mice (E14 to P60). The expression of yy2 reaches a minimum around day P5. Expression levels are normalized to β-actin. (n = 3 sets; * p < 0.05; ** p < 0.01; statistical significances refer to E14).

Mentions: Previously, the expression of yy2, yy1 and mbtps2 in the neocortex and cerebellum of adult mice has been shown by in situ hybridization, but not quantified [7]. To analyze the gene expression during development we used the quantitative real-time PCR technique. As shown in Figure 4 and 5, there were significant changes of yy2 expression from day E14 to P60 by means of TaqMan real-time PCR. By contrast, the yy1 levels remained stable. Interestingly, in cerebellum the amount of yy2 increased up to 6-fold with maturation. In the neocortex yy2 expression significantly decreased during intrauterine gestation, reached a minimum in the early neonatal period, before it increased again in adult mice (P60). However, the content of yy2 transcripts in the hippocampus remained stable on a lower level compared to those of cerebellum and neocortex (Figure 6). In all brain regions, mbtps2 and yy1 were consistently higher expressed (up to 10-fold) than yy2. In contrast to yy2, transcripts of yy1 and mbtps2 were almost constitutively expressed during development. The combined data indicate that yy2 expression underlies a tissue- and developmental-stage specific regulation.


Developmental expression profile of the YY2 gene in mice.

Drews D, Klar M, Dame C, Bräuer AU - BMC Dev. Biol. (2009)

Quantification of yy2, mbtps2 and yy1 in developing neocortex. Quantitative expression analyses (TaqMan real-time PCR) of yy2, mbtps2 and yy1 transcripts in the neocortex during development and in adult mice (E14 to P60). The expression of yy2 reaches a minimum around day P5. Expression levels are normalized to β-actin. (n = 3 sets; * p < 0.05; ** p < 0.01; statistical significances refer to E14).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2724487&req=5

Figure 5: Quantification of yy2, mbtps2 and yy1 in developing neocortex. Quantitative expression analyses (TaqMan real-time PCR) of yy2, mbtps2 and yy1 transcripts in the neocortex during development and in adult mice (E14 to P60). The expression of yy2 reaches a minimum around day P5. Expression levels are normalized to β-actin. (n = 3 sets; * p < 0.05; ** p < 0.01; statistical significances refer to E14).
Mentions: Previously, the expression of yy2, yy1 and mbtps2 in the neocortex and cerebellum of adult mice has been shown by in situ hybridization, but not quantified [7]. To analyze the gene expression during development we used the quantitative real-time PCR technique. As shown in Figure 4 and 5, there were significant changes of yy2 expression from day E14 to P60 by means of TaqMan real-time PCR. By contrast, the yy1 levels remained stable. Interestingly, in cerebellum the amount of yy2 increased up to 6-fold with maturation. In the neocortex yy2 expression significantly decreased during intrauterine gestation, reached a minimum in the early neonatal period, before it increased again in adult mice (P60). However, the content of yy2 transcripts in the hippocampus remained stable on a lower level compared to those of cerebellum and neocortex (Figure 6). In all brain regions, mbtps2 and yy1 were consistently higher expressed (up to 10-fold) than yy2. In contrast to yy2, transcripts of yy1 and mbtps2 were almost constitutively expressed during development. The combined data indicate that yy2 expression underlies a tissue- and developmental-stage specific regulation.

Bottom Line: Again, only yy2 showed significantly altered gene expression levels among the cell types.For the first time, developmental changes of yy2 transcription became obvious in various areas of the brain.This suggests that yy2 is involved in developmental gene regulation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neonatology, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. david.drews@charite.de

ABSTRACT

Background: The transcription factor Yin Yang 2 (YY2) shares a structural and functional highly homologue DNA-binding domain with the ubiquitously expressed YY1 protein, which has been implicated in regulating fundamental biological processes. However, the biological relevance of YY2 has not been identified yet.

Results: Towards the understanding of YY2 biology, we analyzed in detail the expression pattern of yy2 in various organs during embryonic and postnatal mouse development till adulthood. Thereby, a constant yy2 level was detected in heart and lung tissue, whereas in different brain regions yy2 expression was dynamically regulated. Interestingly, in any analyzed tissue neither the homologue yy1 nor the mbtps2 gene showed changes in mRNA expression levels like yy2, although the intronless yy2 gene is located within the mbtps2 locus.Furthermore, we detected yy1, yy2, and mbtps2 mRNA in primary mouse neurons, microglia cells, and astrocytes. In comparison to yy2 and mbtps2, yy1 revealed the highest expression level in all cell types. Again, only yy2 showed significantly altered gene expression levels among the cell types. Higher yy2 expression levels were detected in microglia cells and astrocytes than in primary neurons.

Conclusion: Yy2 expression in the heart and lung is constitutively expressed during embryogenesis and in adult mice. For the first time, developmental changes of yy2 transcription became obvious in various areas of the brain. This suggests that yy2 is involved in developmental gene regulation.

Show MeSH