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Developmental expression profile of the YY2 gene in mice.

Drews D, Klar M, Dame C, Bräuer AU - BMC Dev. Biol. (2009)

Bottom Line: Again, only yy2 showed significantly altered gene expression levels among the cell types.For the first time, developmental changes of yy2 transcription became obvious in various areas of the brain.This suggests that yy2 is involved in developmental gene regulation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neonatology, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. david.drews@charite.de

ABSTRACT

Background: The transcription factor Yin Yang 2 (YY2) shares a structural and functional highly homologue DNA-binding domain with the ubiquitously expressed YY1 protein, which has been implicated in regulating fundamental biological processes. However, the biological relevance of YY2 has not been identified yet.

Results: Towards the understanding of YY2 biology, we analyzed in detail the expression pattern of yy2 in various organs during embryonic and postnatal mouse development till adulthood. Thereby, a constant yy2 level was detected in heart and lung tissue, whereas in different brain regions yy2 expression was dynamically regulated. Interestingly, in any analyzed tissue neither the homologue yy1 nor the mbtps2 gene showed changes in mRNA expression levels like yy2, although the intronless yy2 gene is located within the mbtps2 locus.Furthermore, we detected yy1, yy2, and mbtps2 mRNA in primary mouse neurons, microglia cells, and astrocytes. In comparison to yy2 and mbtps2, yy1 revealed the highest expression level in all cell types. Again, only yy2 showed significantly altered gene expression levels among the cell types. Higher yy2 expression levels were detected in microglia cells and astrocytes than in primary neurons.

Conclusion: Yy2 expression in the heart and lung is constitutively expressed during embryogenesis and in adult mice. For the first time, developmental changes of yy2 transcription became obvious in various areas of the brain. This suggests that yy2 is involved in developmental gene regulation.

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Quantification of yy2, mbtps2 and yy1 in developing lung. Quantification of yy2, mbtps2 and yy1 transcripts levels in the lung during embryogenesis and adult stages (E14 to P30) by TaqMan real-time PCR. The expression data are normalized to β-actin. The pattern is similar to the heart analyzes. (n = 3 sets).
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Figure 3: Quantification of yy2, mbtps2 and yy1 in developing lung. Quantification of yy2, mbtps2 and yy1 transcripts levels in the lung during embryogenesis and adult stages (E14 to P30) by TaqMan real-time PCR. The expression data are normalized to β-actin. The pattern is similar to the heart analyzes. (n = 3 sets).

Mentions: Specimens of the heart and lung, taken at several developmental stages, were dissected, and cDNAs were amplified out of isolated RNA. We analyzed samples spanning from E14 to P60, since in earlier developmental stage region-specific specimens cannot be isolated due to technical limitations. By using TaqMan real-time PCR technique with FAM-labeled probes, we tested the specimens also for yy1 and mbtps2 transcripts. For all three transcripts we found almost stable expression level throughout all tested developmental stages of the heart (Figure 2) and lung (Figure 3). However, in contrast to the highly expressed yy1, the amount of yy2 and mbtps2 transcripts was clearly lower in all developmental stages. Interestingly, yy2 and mbtps2 showed always similar expression levels. Thus, neither the yy genes nor mbtps2 showed any significant changes in expression during heart and lung development.


Developmental expression profile of the YY2 gene in mice.

Drews D, Klar M, Dame C, Bräuer AU - BMC Dev. Biol. (2009)

Quantification of yy2, mbtps2 and yy1 in developing lung. Quantification of yy2, mbtps2 and yy1 transcripts levels in the lung during embryogenesis and adult stages (E14 to P30) by TaqMan real-time PCR. The expression data are normalized to β-actin. The pattern is similar to the heart analyzes. (n = 3 sets).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2724487&req=5

Figure 3: Quantification of yy2, mbtps2 and yy1 in developing lung. Quantification of yy2, mbtps2 and yy1 transcripts levels in the lung during embryogenesis and adult stages (E14 to P30) by TaqMan real-time PCR. The expression data are normalized to β-actin. The pattern is similar to the heart analyzes. (n = 3 sets).
Mentions: Specimens of the heart and lung, taken at several developmental stages, were dissected, and cDNAs were amplified out of isolated RNA. We analyzed samples spanning from E14 to P60, since in earlier developmental stage region-specific specimens cannot be isolated due to technical limitations. By using TaqMan real-time PCR technique with FAM-labeled probes, we tested the specimens also for yy1 and mbtps2 transcripts. For all three transcripts we found almost stable expression level throughout all tested developmental stages of the heart (Figure 2) and lung (Figure 3). However, in contrast to the highly expressed yy1, the amount of yy2 and mbtps2 transcripts was clearly lower in all developmental stages. Interestingly, yy2 and mbtps2 showed always similar expression levels. Thus, neither the yy genes nor mbtps2 showed any significant changes in expression during heart and lung development.

Bottom Line: Again, only yy2 showed significantly altered gene expression levels among the cell types.For the first time, developmental changes of yy2 transcription became obvious in various areas of the brain.This suggests that yy2 is involved in developmental gene regulation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neonatology, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. david.drews@charite.de

ABSTRACT

Background: The transcription factor Yin Yang 2 (YY2) shares a structural and functional highly homologue DNA-binding domain with the ubiquitously expressed YY1 protein, which has been implicated in regulating fundamental biological processes. However, the biological relevance of YY2 has not been identified yet.

Results: Towards the understanding of YY2 biology, we analyzed in detail the expression pattern of yy2 in various organs during embryonic and postnatal mouse development till adulthood. Thereby, a constant yy2 level was detected in heart and lung tissue, whereas in different brain regions yy2 expression was dynamically regulated. Interestingly, in any analyzed tissue neither the homologue yy1 nor the mbtps2 gene showed changes in mRNA expression levels like yy2, although the intronless yy2 gene is located within the mbtps2 locus.Furthermore, we detected yy1, yy2, and mbtps2 mRNA in primary mouse neurons, microglia cells, and astrocytes. In comparison to yy2 and mbtps2, yy1 revealed the highest expression level in all cell types. Again, only yy2 showed significantly altered gene expression levels among the cell types. Higher yy2 expression levels were detected in microglia cells and astrocytes than in primary neurons.

Conclusion: Yy2 expression in the heart and lung is constitutively expressed during embryogenesis and in adult mice. For the first time, developmental changes of yy2 transcription became obvious in various areas of the brain. This suggests that yy2 is involved in developmental gene regulation.

Show MeSH