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The effect of caffeic acid phenethyl ester on the functions of human monocyte-derived dendritic cells.

Wang LC, Lin YL, Liang YC, Yang YH, Lee JH, Yu HH, Wu WM, Chiang BL - BMC Immunol. (2009)

Bottom Line: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract.CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract.These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan, Republic of China.lcwang5@ntu.edu.tw

ABSTRACT

Background: Propolis, an ancient herbal medicine, has been reported the beneficial effect both in asthma patients and murine model of asthma, but the mechanism was not clearly understood. In this study, the effect of caffeic acid phenethyl ester (CAPE), the most extensively studied components in propolis, on the functions of human monocyte-derived dendritic cells (MoDCs) was investigated.

Results: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract. CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract. In contrast, the upregulation of costimulatory molecules in mature MoDCs was not suppressed by CAPE. Further, the antigen presenting ability of DCs was not inhibited by CAPE. CAPE inhibited IkappaBalpha phosphorylation and NF-kappaB activation but not mitogen-activated protein kinase (MAPK) family phosphorylation in human MoDCs.

Conclusion: These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway. This study provided a new insight into the mechanism of CAPE in immune response and the rationale for propolis in the treatment of asthma and other allergic disorders.

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Related in: MedlinePlus

The effect of CAPE on the phagocytic capacity of human MoDCs. Immature MoDCs from healthy subjects were cultured for 24 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Cells were then incubated with FITC-dextran for 1 hour at 4°C (thin lines) or 37°C (thick lines). The values shown in the flow cytometry profiles are the MFI indexes. One representative of three independent experiments is shown.
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Figure 3: The effect of CAPE on the phagocytic capacity of human MoDCs. Immature MoDCs from healthy subjects were cultured for 24 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Cells were then incubated with FITC-dextran for 1 hour at 4°C (thin lines) or 37°C (thick lines). The values shown in the flow cytometry profiles are the MFI indexes. One representative of three independent experiments is shown.

Mentions: Immature DCs capture antigens through phagocytosis, macropinocytosis and adsorptive endocytosis. Then they become mature DCs and lose their ability of antigen uptake [31]. The uptake of FITC-dextran is known to be maximal in the immature human MoDCs through macropinocytosis and mannose receptor [32]. To determine whether CAPE could modulate the antigen uptake ability of human MoDCs, FITC-dextran was analyzed by flow cytometry (Figure 3). In healthy subjects, the LPS and crude mite extract-treated MoDCs had decreased ability of FITC-dextran uptake compared to control MoDCs. However, CAPE did not alter the phagocytic capacity in human MoDCs.


The effect of caffeic acid phenethyl ester on the functions of human monocyte-derived dendritic cells.

Wang LC, Lin YL, Liang YC, Yang YH, Lee JH, Yu HH, Wu WM, Chiang BL - BMC Immunol. (2009)

The effect of CAPE on the phagocytic capacity of human MoDCs. Immature MoDCs from healthy subjects were cultured for 24 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Cells were then incubated with FITC-dextran for 1 hour at 4°C (thin lines) or 37°C (thick lines). The values shown in the flow cytometry profiles are the MFI indexes. One representative of three independent experiments is shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2724478&req=5

Figure 3: The effect of CAPE on the phagocytic capacity of human MoDCs. Immature MoDCs from healthy subjects were cultured for 24 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Cells were then incubated with FITC-dextran for 1 hour at 4°C (thin lines) or 37°C (thick lines). The values shown in the flow cytometry profiles are the MFI indexes. One representative of three independent experiments is shown.
Mentions: Immature DCs capture antigens through phagocytosis, macropinocytosis and adsorptive endocytosis. Then they become mature DCs and lose their ability of antigen uptake [31]. The uptake of FITC-dextran is known to be maximal in the immature human MoDCs through macropinocytosis and mannose receptor [32]. To determine whether CAPE could modulate the antigen uptake ability of human MoDCs, FITC-dextran was analyzed by flow cytometry (Figure 3). In healthy subjects, the LPS and crude mite extract-treated MoDCs had decreased ability of FITC-dextran uptake compared to control MoDCs. However, CAPE did not alter the phagocytic capacity in human MoDCs.

Bottom Line: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract.CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract.These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan, Republic of China.lcwang5@ntu.edu.tw

ABSTRACT

Background: Propolis, an ancient herbal medicine, has been reported the beneficial effect both in asthma patients and murine model of asthma, but the mechanism was not clearly understood. In this study, the effect of caffeic acid phenethyl ester (CAPE), the most extensively studied components in propolis, on the functions of human monocyte-derived dendritic cells (MoDCs) was investigated.

Results: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract. CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract. In contrast, the upregulation of costimulatory molecules in mature MoDCs was not suppressed by CAPE. Further, the antigen presenting ability of DCs was not inhibited by CAPE. CAPE inhibited IkappaBalpha phosphorylation and NF-kappaB activation but not mitogen-activated protein kinase (MAPK) family phosphorylation in human MoDCs.

Conclusion: These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway. This study provided a new insight into the mechanism of CAPE in immune response and the rationale for propolis in the treatment of asthma and other allergic disorders.

Show MeSH
Related in: MedlinePlus