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The effect of caffeic acid phenethyl ester on the functions of human monocyte-derived dendritic cells.

Wang LC, Lin YL, Liang YC, Yang YH, Lee JH, Yu HH, Wu WM, Chiang BL - BMC Immunol. (2009)

Bottom Line: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract.CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract.These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan, Republic of China.lcwang5@ntu.edu.tw

ABSTRACT

Background: Propolis, an ancient herbal medicine, has been reported the beneficial effect both in asthma patients and murine model of asthma, but the mechanism was not clearly understood. In this study, the effect of caffeic acid phenethyl ester (CAPE), the most extensively studied components in propolis, on the functions of human monocyte-derived dendritic cells (MoDCs) was investigated.

Results: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract. CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract. In contrast, the upregulation of costimulatory molecules in mature MoDCs was not suppressed by CAPE. Further, the antigen presenting ability of DCs was not inhibited by CAPE. CAPE inhibited IkappaBalpha phosphorylation and NF-kappaB activation but not mitogen-activated protein kinase (MAPK) family phosphorylation in human MoDCs.

Conclusion: These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway. This study provided a new insight into the mechanism of CAPE in immune response and the rationale for propolis in the treatment of asthma and other allergic disorders.

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CAPE did not inhibit costimulatory molecule (such as CD86, CD80, and CD83) expression on MoDCs. Immature MoDCs from healthy subjects were cultured for 48 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Surface markers were analyzed by flow cytometry. (A) Histogram of fluorescence intensity of HLA-DR, CD86, CD80 and CD83. The values shown in the flow cytometry profiles are the mean fluorescence intensity (MFI) indexes. (B) MFI of HLA-DR, CD86, CD80, and CD83. Data shown represent mean ± SEM from three separate experiments. *P < 0.05; **P < 0.01.
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Figure 2: CAPE did not inhibit costimulatory molecule (such as CD86, CD80, and CD83) expression on MoDCs. Immature MoDCs from healthy subjects were cultured for 48 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Surface markers were analyzed by flow cytometry. (A) Histogram of fluorescence intensity of HLA-DR, CD86, CD80 and CD83. The values shown in the flow cytometry profiles are the mean fluorescence intensity (MFI) indexes. (B) MFI of HLA-DR, CD86, CD80, and CD83. Data shown represent mean ± SEM from three separate experiments. *P < 0.05; **P < 0.01.

Mentions: To determine whether CAPE could modulate the maturation of human MoDCs in vitro, the phenotype of MoDCs from healthy subjects treated as described above was assessed via analysis of surface markers by flow cytometry (Figure 2). Compared to control MoDCs, MoDCs treated with LPS had increased expression of HLA-DR, CD86, CD80 and CD83 (P = 0.007, 0.005, <0.001 and 0.006 respectively) and MoDCs treated with crude mite extract had increased expression of HLA-DR, CD86 and CD80 (P = 0.007, 0.018 and 0.020, respectively). In contrast, the costimulatory molecules (CD86, CD80, CD83) and MHC class II molecules were not significantly inhibited by CAPE treatment.


The effect of caffeic acid phenethyl ester on the functions of human monocyte-derived dendritic cells.

Wang LC, Lin YL, Liang YC, Yang YH, Lee JH, Yu HH, Wu WM, Chiang BL - BMC Immunol. (2009)

CAPE did not inhibit costimulatory molecule (such as CD86, CD80, and CD83) expression on MoDCs. Immature MoDCs from healthy subjects were cultured for 48 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Surface markers were analyzed by flow cytometry. (A) Histogram of fluorescence intensity of HLA-DR, CD86, CD80 and CD83. The values shown in the flow cytometry profiles are the mean fluorescence intensity (MFI) indexes. (B) MFI of HLA-DR, CD86, CD80, and CD83. Data shown represent mean ± SEM from three separate experiments. *P < 0.05; **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2724478&req=5

Figure 2: CAPE did not inhibit costimulatory molecule (such as CD86, CD80, and CD83) expression on MoDCs. Immature MoDCs from healthy subjects were cultured for 48 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Surface markers were analyzed by flow cytometry. (A) Histogram of fluorescence intensity of HLA-DR, CD86, CD80 and CD83. The values shown in the flow cytometry profiles are the mean fluorescence intensity (MFI) indexes. (B) MFI of HLA-DR, CD86, CD80, and CD83. Data shown represent mean ± SEM from three separate experiments. *P < 0.05; **P < 0.01.
Mentions: To determine whether CAPE could modulate the maturation of human MoDCs in vitro, the phenotype of MoDCs from healthy subjects treated as described above was assessed via analysis of surface markers by flow cytometry (Figure 2). Compared to control MoDCs, MoDCs treated with LPS had increased expression of HLA-DR, CD86, CD80 and CD83 (P = 0.007, 0.005, <0.001 and 0.006 respectively) and MoDCs treated with crude mite extract had increased expression of HLA-DR, CD86 and CD80 (P = 0.007, 0.018 and 0.020, respectively). In contrast, the costimulatory molecules (CD86, CD80, CD83) and MHC class II molecules were not significantly inhibited by CAPE treatment.

Bottom Line: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract.CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract.These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan, Republic of China.lcwang5@ntu.edu.tw

ABSTRACT

Background: Propolis, an ancient herbal medicine, has been reported the beneficial effect both in asthma patients and murine model of asthma, but the mechanism was not clearly understood. In this study, the effect of caffeic acid phenethyl ester (CAPE), the most extensively studied components in propolis, on the functions of human monocyte-derived dendritic cells (MoDCs) was investigated.

Results: CAPE significantly inhibited IL-12 p40, IL-12 p70, IL-10 protein expression in mature healthy human MoDCs stimulated by lipopolysaccharides (LPS) and IL-12 p40, IL-10, IP-10 stimulated by crude mite extract. CAPE significantly inhibited IL-10 and IP-10 but not IL-12 expression in allergic patients' MoDCs stimulated by crude mite extract. In contrast, the upregulation of costimulatory molecules in mature MoDCs was not suppressed by CAPE. Further, the antigen presenting ability of DCs was not inhibited by CAPE. CAPE inhibited IkappaBalpha phosphorylation and NF-kappaB activation but not mitogen-activated protein kinase (MAPK) family phosphorylation in human MoDCs.

Conclusion: These results indicated that CAPE inhibited cytokine and chemokine production by MoDCs which might be related to the NF-kappaB signaling pathway. This study provided a new insight into the mechanism of CAPE in immune response and the rationale for propolis in the treatment of asthma and other allergic disorders.

Show MeSH
Related in: MedlinePlus