Limits...
Detection of differentially expressed genes between Erhualian and Large White placentas on day 75 and 90 of gestation.

Zhou QY, Fang MD, Huang TH, Li CC, Yu M, Zhao SH - BMC Genomics (2009)

Bottom Line: Placental efficiency is strongly associated with litter size, fetal weight and prenatal mortality.Together with its rapid growth during late gestation, the Large White pig breed shows a significant increase in placental size and weight, but this does not occur in the highly prolific Chinese pig breeds.Differential expression of six candidate imprinted genes was also confirmed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, PR China. zhouquanyong@webmail.hzau.edu.cn

ABSTRACT

Background: Placental efficiency is strongly associated with litter size, fetal weight and prenatal mortality. Together with its rapid growth during late gestation, the Large White pig breed shows a significant increase in placental size and weight, but this does not occur in the highly prolific Chinese pig breeds. To understand the molecular basis of placental development during late gestation in Chinese indigenous and Western breeds with different placental efficiency, female placental samples were collected from six pregnant Erhualian gilts at gestation day 75 (E75) and day 90 (E90) and from six pregnant Large White gilts at gestation day 75 (L75) and day 90 (L90). Two female placentas from one sow were used to extract RNA and then pooled in equal volumes. Twelve pooled samples were hybridized to the porcine Affymetrix GeneChip.

Results: A total of 226 and 577 transcripts were detected that were differentially expressed between E75 and L75 and between E90 and L90 (p < 0.01, q < 0.2), respectively. Gene Ontology (GO) analysis revealed that these genes belong to the class of genes that participate in angiogenesis and development. Real-time RT-PCR confirmed the differential expression of eight selected genes. Significant differential expression of five genes in the VEGF pathway was also detected between the breeds. A search of chromosomal location revealed that 44 differentially expressed genes located to QTL regions related to reproduction. Differential expression of six candidate imprinted genes was also confirmed. Three of the six genes (PLAGL1, DIRAS3, and SLC38A4) showed monoallelic expression in the porcine placenta.

Conclusion: Our study detected many genes that showed differential expression between placentas of two divergent breed of pigs, and confirmed the imprinting of three genes. These findings help to elucidate the genetic control of placental efficiency and improve the understanding of placental development.

Show MeSH
Gene Ontology (GO) molecular function classification of all genes expressed in placenta and differentially expressed genes. (A) All expressed genes in placenta. (B) Differentially expressed genes in E75 vs L75. (C) Differentially expressed genes in E90 vs L90. The x-axis shows the gene percentage of each GO category with regard to the placenta transcripts or the declared differentially expressed genes and the y-axis represents each GO category.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2724418&req=5

Figure 1: Gene Ontology (GO) molecular function classification of all genes expressed in placenta and differentially expressed genes. (A) All expressed genes in placenta. (B) Differentially expressed genes in E75 vs L75. (C) Differentially expressed genes in E90 vs L90. The x-axis shows the gene percentage of each GO category with regard to the placenta transcripts or the declared differentially expressed genes and the y-axis represents each GO category.

Mentions: There are 24,123 probesets, which represent 20,201 transcripts and 198 controls, on the Affymetrix porcine GeneChip. The transcriptome analysis indicated that 7,842 probesets were expressed in the placenta (GEO accession numbers: GSM299411-GSM299422). Through BLAST sequence similarity analyses, 6087 transcripts were matched to human Refseq entries. GO annotation was used to classify these genes into groups representing different biological processes. The results revealed that these genes were involved in metabolic processes, transport and developmental process, etc. (Figure 1A). It is interesting that many genes had high and stable expression in E75, E90, L75 and L90. The strong expression of these genes in the placenta suggests that they may have important roles in placental development or nutrient transportation. We further checked the relative expression of some homologous genes in data obtained from human . Some genes that are specifically expressed in the placenta (EBI3, HSD3B1, HSD17B1), or are specifically expressed in a few tissues including the placenta, in other species [14], such as FOXF2, COL1A1, COL3A1, PERP, SPP1 and PAPPA, were detected to have high expression in our study.


Detection of differentially expressed genes between Erhualian and Large White placentas on day 75 and 90 of gestation.

Zhou QY, Fang MD, Huang TH, Li CC, Yu M, Zhao SH - BMC Genomics (2009)

Gene Ontology (GO) molecular function classification of all genes expressed in placenta and differentially expressed genes. (A) All expressed genes in placenta. (B) Differentially expressed genes in E75 vs L75. (C) Differentially expressed genes in E90 vs L90. The x-axis shows the gene percentage of each GO category with regard to the placenta transcripts or the declared differentially expressed genes and the y-axis represents each GO category.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2724418&req=5

Figure 1: Gene Ontology (GO) molecular function classification of all genes expressed in placenta and differentially expressed genes. (A) All expressed genes in placenta. (B) Differentially expressed genes in E75 vs L75. (C) Differentially expressed genes in E90 vs L90. The x-axis shows the gene percentage of each GO category with regard to the placenta transcripts or the declared differentially expressed genes and the y-axis represents each GO category.
Mentions: There are 24,123 probesets, which represent 20,201 transcripts and 198 controls, on the Affymetrix porcine GeneChip. The transcriptome analysis indicated that 7,842 probesets were expressed in the placenta (GEO accession numbers: GSM299411-GSM299422). Through BLAST sequence similarity analyses, 6087 transcripts were matched to human Refseq entries. GO annotation was used to classify these genes into groups representing different biological processes. The results revealed that these genes were involved in metabolic processes, transport and developmental process, etc. (Figure 1A). It is interesting that many genes had high and stable expression in E75, E90, L75 and L90. The strong expression of these genes in the placenta suggests that they may have important roles in placental development or nutrient transportation. We further checked the relative expression of some homologous genes in data obtained from human . Some genes that are specifically expressed in the placenta (EBI3, HSD3B1, HSD17B1), or are specifically expressed in a few tissues including the placenta, in other species [14], such as FOXF2, COL1A1, COL3A1, PERP, SPP1 and PAPPA, were detected to have high expression in our study.

Bottom Line: Placental efficiency is strongly associated with litter size, fetal weight and prenatal mortality.Together with its rapid growth during late gestation, the Large White pig breed shows a significant increase in placental size and weight, but this does not occur in the highly prolific Chinese pig breeds.Differential expression of six candidate imprinted genes was also confirmed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, PR China. zhouquanyong@webmail.hzau.edu.cn

ABSTRACT

Background: Placental efficiency is strongly associated with litter size, fetal weight and prenatal mortality. Together with its rapid growth during late gestation, the Large White pig breed shows a significant increase in placental size and weight, but this does not occur in the highly prolific Chinese pig breeds. To understand the molecular basis of placental development during late gestation in Chinese indigenous and Western breeds with different placental efficiency, female placental samples were collected from six pregnant Erhualian gilts at gestation day 75 (E75) and day 90 (E90) and from six pregnant Large White gilts at gestation day 75 (L75) and day 90 (L90). Two female placentas from one sow were used to extract RNA and then pooled in equal volumes. Twelve pooled samples were hybridized to the porcine Affymetrix GeneChip.

Results: A total of 226 and 577 transcripts were detected that were differentially expressed between E75 and L75 and between E90 and L90 (p < 0.01, q < 0.2), respectively. Gene Ontology (GO) analysis revealed that these genes belong to the class of genes that participate in angiogenesis and development. Real-time RT-PCR confirmed the differential expression of eight selected genes. Significant differential expression of five genes in the VEGF pathway was also detected between the breeds. A search of chromosomal location revealed that 44 differentially expressed genes located to QTL regions related to reproduction. Differential expression of six candidate imprinted genes was also confirmed. Three of the six genes (PLAGL1, DIRAS3, and SLC38A4) showed monoallelic expression in the porcine placenta.

Conclusion: Our study detected many genes that showed differential expression between placentas of two divergent breed of pigs, and confirmed the imprinting of three genes. These findings help to elucidate the genetic control of placental efficiency and improve the understanding of placental development.

Show MeSH