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Jasmonate-dependent plant defense restricts thrips performance and preference.

Abe H, Shimoda T, Ohnishi J, Kugimiya S, Narusaka M, Seo S, Narusaka Y, Tsuda S, Kobayashi M - BMC Plant Biol. (2009)

Bottom Line: Application of JA to WT plants before thrips attack decreased the thrips population.Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa.Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Plant Division, RIKEN BioResource Center, Tsukuba 305-0074, Japan. ahiroshi@rtc.riken.jp

ABSTRACT

Background: The western flower thrips (Frankliniella occidentalis [Pergande]) is one of the most important insect herbivores of cultivated plants. However, no pesticide provides complete control of this species, and insecticide resistance has emerged around the world. We previously reported the important role of jasmonate (JA) in the plant's immediate response to thrips feeding by using an Arabidopsis leaf disc system. In this study, as the first step toward practical use of JA in thrips control, we analyzed the effect of JA-regulated Arabidopsis defense at the whole plant level on thrips behavior and life cycle at the population level over an extended period. We also studied the effectiveness of JA-regulated plant defense on thrips damage in Chinese cabbage (Brassica rapa subsp. pekinensis).

Results: Thrips oviposited more on Arabidopsis JA-insensitive coi1-1 mutants than on WT plants, and the population density of the following thrips generation increased on coi1-1 mutants. Moreover, thrips preferred coi1-1 mutants more than WT plants. Application of JA to WT plants before thrips attack decreased the thrips population. To analyze these important functions of JA in a brassica crop plant, we analyzed the expression of marker genes for JA response in B. rapa. Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa. Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

Conclusion: Our results indicate that the JA-regulated plant defense restricts thrips performance and preference, and plays an important role in the resistance of Arabidopsis and B. rapa to thrips damage.

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Effect of JA-induced B. rapa defense response on thrips population. (A) Water (control) or 10, 100, or 1000 μM JA was applied to 2-week-old B. rapa plants 1 day before thrips were introduced. One adult female fed on each leaf disc for 4 days. Eggs were stained with trypan blue. Mean ± SD of eggs per leaf disc based on 10 independent determinations. Different letters indicate statistically significant differences between treatments (Tukey-Kramer HSD test; p < 0.05). (B, C) Twenty adult females fed on 2-week-old WT plants for 2 weeks. Water (control) or 50 μM JA was applied 1 day before thrips were introduced. After 2 weeks, adults (B) and larvae (C) were counted. Mean ± SD based on five independent determinations. Asterisks indicate significant differences (Student's t-test), *p < 0.05, ***p < 0.001.
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Figure 8: Effect of JA-induced B. rapa defense response on thrips population. (A) Water (control) or 10, 100, or 1000 μM JA was applied to 2-week-old B. rapa plants 1 day before thrips were introduced. One adult female fed on each leaf disc for 4 days. Eggs were stained with trypan blue. Mean ± SD of eggs per leaf disc based on 10 independent determinations. Different letters indicate statistically significant differences between treatments (Tukey-Kramer HSD test; p < 0.05). (B, C) Twenty adult females fed on 2-week-old WT plants for 2 weeks. Water (control) or 50 μM JA was applied 1 day before thrips were introduced. After 2 weeks, adults (B) and larvae (C) were counted. Mean ± SD based on five independent determinations. Asterisks indicate significant differences (Student's t-test), *p < 0.05, ***p < 0.001.

Mentions: We further analyzed JA's effect on thrips oviposition. Rosette leaves of B. rapa were cut into leaf discs with 8-mm diameter. One adult female thrips was put on each leaf disc and allowed to feed and oviposit for 4 days. Application of JA dose-dependently decreased the number of eggs (one-way ANOVA, F = 10.367, df = 4, p < 0.001; Fig. 8A). Finally, we analyzed the effect of JA on the next generation. JA treatment of plants restrained the thrips population very effectively (Fig. 8B, C). These results clearly indicate the important role of JA in resistance to thrips attack in B. rapa also.


Jasmonate-dependent plant defense restricts thrips performance and preference.

Abe H, Shimoda T, Ohnishi J, Kugimiya S, Narusaka M, Seo S, Narusaka Y, Tsuda S, Kobayashi M - BMC Plant Biol. (2009)

Effect of JA-induced B. rapa defense response on thrips population. (A) Water (control) or 10, 100, or 1000 μM JA was applied to 2-week-old B. rapa plants 1 day before thrips were introduced. One adult female fed on each leaf disc for 4 days. Eggs were stained with trypan blue. Mean ± SD of eggs per leaf disc based on 10 independent determinations. Different letters indicate statistically significant differences between treatments (Tukey-Kramer HSD test; p < 0.05). (B, C) Twenty adult females fed on 2-week-old WT plants for 2 weeks. Water (control) or 50 μM JA was applied 1 day before thrips were introduced. After 2 weeks, adults (B) and larvae (C) were counted. Mean ± SD based on five independent determinations. Asterisks indicate significant differences (Student's t-test), *p < 0.05, ***p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2724403&req=5

Figure 8: Effect of JA-induced B. rapa defense response on thrips population. (A) Water (control) or 10, 100, or 1000 μM JA was applied to 2-week-old B. rapa plants 1 day before thrips were introduced. One adult female fed on each leaf disc for 4 days. Eggs were stained with trypan blue. Mean ± SD of eggs per leaf disc based on 10 independent determinations. Different letters indicate statistically significant differences between treatments (Tukey-Kramer HSD test; p < 0.05). (B, C) Twenty adult females fed on 2-week-old WT plants for 2 weeks. Water (control) or 50 μM JA was applied 1 day before thrips were introduced. After 2 weeks, adults (B) and larvae (C) were counted. Mean ± SD based on five independent determinations. Asterisks indicate significant differences (Student's t-test), *p < 0.05, ***p < 0.001.
Mentions: We further analyzed JA's effect on thrips oviposition. Rosette leaves of B. rapa were cut into leaf discs with 8-mm diameter. One adult female thrips was put on each leaf disc and allowed to feed and oviposit for 4 days. Application of JA dose-dependently decreased the number of eggs (one-way ANOVA, F = 10.367, df = 4, p < 0.001; Fig. 8A). Finally, we analyzed the effect of JA on the next generation. JA treatment of plants restrained the thrips population very effectively (Fig. 8B, C). These results clearly indicate the important role of JA in resistance to thrips attack in B. rapa also.

Bottom Line: Application of JA to WT plants before thrips attack decreased the thrips population.Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa.Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Plant Division, RIKEN BioResource Center, Tsukuba 305-0074, Japan. ahiroshi@rtc.riken.jp

ABSTRACT

Background: The western flower thrips (Frankliniella occidentalis [Pergande]) is one of the most important insect herbivores of cultivated plants. However, no pesticide provides complete control of this species, and insecticide resistance has emerged around the world. We previously reported the important role of jasmonate (JA) in the plant's immediate response to thrips feeding by using an Arabidopsis leaf disc system. In this study, as the first step toward practical use of JA in thrips control, we analyzed the effect of JA-regulated Arabidopsis defense at the whole plant level on thrips behavior and life cycle at the population level over an extended period. We also studied the effectiveness of JA-regulated plant defense on thrips damage in Chinese cabbage (Brassica rapa subsp. pekinensis).

Results: Thrips oviposited more on Arabidopsis JA-insensitive coi1-1 mutants than on WT plants, and the population density of the following thrips generation increased on coi1-1 mutants. Moreover, thrips preferred coi1-1 mutants more than WT plants. Application of JA to WT plants before thrips attack decreased the thrips population. To analyze these important functions of JA in a brassica crop plant, we analyzed the expression of marker genes for JA response in B. rapa. Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa. Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

Conclusion: Our results indicate that the JA-regulated plant defense restricts thrips performance and preference, and plays an important role in the resistance of Arabidopsis and B. rapa to thrips damage.

Show MeSH
Related in: MedlinePlus