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Jasmonate-dependent plant defense restricts thrips performance and preference.

Abe H, Shimoda T, Ohnishi J, Kugimiya S, Narusaka M, Seo S, Narusaka Y, Tsuda S, Kobayashi M - BMC Plant Biol. (2009)

Bottom Line: Application of JA to WT plants before thrips attack decreased the thrips population.Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa.Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Plant Division, RIKEN BioResource Center, Tsukuba 305-0074, Japan. ahiroshi@rtc.riken.jp

ABSTRACT

Background: The western flower thrips (Frankliniella occidentalis [Pergande]) is one of the most important insect herbivores of cultivated plants. However, no pesticide provides complete control of this species, and insecticide resistance has emerged around the world. We previously reported the important role of jasmonate (JA) in the plant's immediate response to thrips feeding by using an Arabidopsis leaf disc system. In this study, as the first step toward practical use of JA in thrips control, we analyzed the effect of JA-regulated Arabidopsis defense at the whole plant level on thrips behavior and life cycle at the population level over an extended period. We also studied the effectiveness of JA-regulated plant defense on thrips damage in Chinese cabbage (Brassica rapa subsp. pekinensis).

Results: Thrips oviposited more on Arabidopsis JA-insensitive coi1-1 mutants than on WT plants, and the population density of the following thrips generation increased on coi1-1 mutants. Moreover, thrips preferred coi1-1 mutants more than WT plants. Application of JA to WT plants before thrips attack decreased the thrips population. To analyze these important functions of JA in a brassica crop plant, we analyzed the expression of marker genes for JA response in B. rapa. Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa. Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

Conclusion: Our results indicate that the JA-regulated plant defense restricts thrips performance and preference, and plays an important role in the resistance of Arabidopsis and B. rapa to thrips damage.

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Related in: MedlinePlus

Effect of JA-dependent plant resistance on thrips oviposition on leaf discs. One adult female fed per leaf disc of 3-week-old WT plants (A) or coi1-1 mutants (B) for 4 days. Eggs oviposited on leaf discs were stained with trypan blue. Photos show typical leaf discs after staining; some eggs are shown by red arrowheads. (C) Number of eggs per leaf disc (mean ± SD) based on more than 10 independent determinations. Asterisks indicate significant difference (Student's t-test), ***p < 0.001.
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Figure 2: Effect of JA-dependent plant resistance on thrips oviposition on leaf discs. One adult female fed per leaf disc of 3-week-old WT plants (A) or coi1-1 mutants (B) for 4 days. Eggs oviposited on leaf discs were stained with trypan blue. Photos show typical leaf discs after staining; some eggs are shown by red arrowheads. (C) Number of eggs per leaf disc (mean ± SD) based on more than 10 independent determinations. Asterisks indicate significant difference (Student's t-test), ***p < 0.001.

Mentions: To understand why coi1-1 mutants showed low resistance to thrips attack, we first analyzed the number of thrips eggs on the WT plants and coi1-1 mutants to compare the asexual oviposition performance of thrips. Arabidopsis rosette leaves were cut into leaf discs with 8-mm diameter. One adult female thrips was put on each disc and allowed to feed and oviposit. Because the females lay in the epidermal or mesophyll cells [29], we stained the eggs with trypan blue to count them. As we reported previously [23], the area of feeding scars on coi1-1 mutants was greater than that on WT plants (data not shown). The number of eggs on the coi1-1 discs was double that on the WT discs (Fig. 2A–C). The decreased resistance of these coi1-1 mutants could explain the increased oviposition rate on these plants.


Jasmonate-dependent plant defense restricts thrips performance and preference.

Abe H, Shimoda T, Ohnishi J, Kugimiya S, Narusaka M, Seo S, Narusaka Y, Tsuda S, Kobayashi M - BMC Plant Biol. (2009)

Effect of JA-dependent plant resistance on thrips oviposition on leaf discs. One adult female fed per leaf disc of 3-week-old WT plants (A) or coi1-1 mutants (B) for 4 days. Eggs oviposited on leaf discs were stained with trypan blue. Photos show typical leaf discs after staining; some eggs are shown by red arrowheads. (C) Number of eggs per leaf disc (mean ± SD) based on more than 10 independent determinations. Asterisks indicate significant difference (Student's t-test), ***p < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2724403&req=5

Figure 2: Effect of JA-dependent plant resistance on thrips oviposition on leaf discs. One adult female fed per leaf disc of 3-week-old WT plants (A) or coi1-1 mutants (B) for 4 days. Eggs oviposited on leaf discs were stained with trypan blue. Photos show typical leaf discs after staining; some eggs are shown by red arrowheads. (C) Number of eggs per leaf disc (mean ± SD) based on more than 10 independent determinations. Asterisks indicate significant difference (Student's t-test), ***p < 0.001.
Mentions: To understand why coi1-1 mutants showed low resistance to thrips attack, we first analyzed the number of thrips eggs on the WT plants and coi1-1 mutants to compare the asexual oviposition performance of thrips. Arabidopsis rosette leaves were cut into leaf discs with 8-mm diameter. One adult female thrips was put on each disc and allowed to feed and oviposit. Because the females lay in the epidermal or mesophyll cells [29], we stained the eggs with trypan blue to count them. As we reported previously [23], the area of feeding scars on coi1-1 mutants was greater than that on WT plants (data not shown). The number of eggs on the coi1-1 discs was double that on the WT discs (Fig. 2A–C). The decreased resistance of these coi1-1 mutants could explain the increased oviposition rate on these plants.

Bottom Line: Application of JA to WT plants before thrips attack decreased the thrips population.Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa.Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Plant Division, RIKEN BioResource Center, Tsukuba 305-0074, Japan. ahiroshi@rtc.riken.jp

ABSTRACT

Background: The western flower thrips (Frankliniella occidentalis [Pergande]) is one of the most important insect herbivores of cultivated plants. However, no pesticide provides complete control of this species, and insecticide resistance has emerged around the world. We previously reported the important role of jasmonate (JA) in the plant's immediate response to thrips feeding by using an Arabidopsis leaf disc system. In this study, as the first step toward practical use of JA in thrips control, we analyzed the effect of JA-regulated Arabidopsis defense at the whole plant level on thrips behavior and life cycle at the population level over an extended period. We also studied the effectiveness of JA-regulated plant defense on thrips damage in Chinese cabbage (Brassica rapa subsp. pekinensis).

Results: Thrips oviposited more on Arabidopsis JA-insensitive coi1-1 mutants than on WT plants, and the population density of the following thrips generation increased on coi1-1 mutants. Moreover, thrips preferred coi1-1 mutants more than WT plants. Application of JA to WT plants before thrips attack decreased the thrips population. To analyze these important functions of JA in a brassica crop plant, we analyzed the expression of marker genes for JA response in B. rapa. Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa. Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

Conclusion: Our results indicate that the JA-regulated plant defense restricts thrips performance and preference, and plays an important role in the resistance of Arabidopsis and B. rapa to thrips damage.

Show MeSH
Related in: MedlinePlus