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Jasmonate-dependent plant defense restricts thrips performance and preference.

Abe H, Shimoda T, Ohnishi J, Kugimiya S, Narusaka M, Seo S, Narusaka Y, Tsuda S, Kobayashi M - BMC Plant Biol. (2009)

Bottom Line: Application of JA to WT plants before thrips attack decreased the thrips population.Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa.Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Plant Division, RIKEN BioResource Center, Tsukuba 305-0074, Japan. ahiroshi@rtc.riken.jp

ABSTRACT

Background: The western flower thrips (Frankliniella occidentalis [Pergande]) is one of the most important insect herbivores of cultivated plants. However, no pesticide provides complete control of this species, and insecticide resistance has emerged around the world. We previously reported the important role of jasmonate (JA) in the plant's immediate response to thrips feeding by using an Arabidopsis leaf disc system. In this study, as the first step toward practical use of JA in thrips control, we analyzed the effect of JA-regulated Arabidopsis defense at the whole plant level on thrips behavior and life cycle at the population level over an extended period. We also studied the effectiveness of JA-regulated plant defense on thrips damage in Chinese cabbage (Brassica rapa subsp. pekinensis).

Results: Thrips oviposited more on Arabidopsis JA-insensitive coi1-1 mutants than on WT plants, and the population density of the following thrips generation increased on coi1-1 mutants. Moreover, thrips preferred coi1-1 mutants more than WT plants. Application of JA to WT plants before thrips attack decreased the thrips population. To analyze these important functions of JA in a brassica crop plant, we analyzed the expression of marker genes for JA response in B. rapa. Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa. Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

Conclusion: Our results indicate that the JA-regulated plant defense restricts thrips performance and preference, and plays an important role in the resistance of Arabidopsis and B. rapa to thrips damage.

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Function of JA in plant resistance to thrips feeding. Twenty adult females fed on 3-week-old WT plants (left) or coi1-1 mutants (right). Typical plants after 4 weeks of feeding are shown.
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Figure 1: Function of JA in plant resistance to thrips feeding. Twenty adult females fed on 3-week-old WT plants (left) or coi1-1 mutants (right). Typical plants after 4 weeks of feeding are shown.

Mentions: We recently reported the role of JA in the short-term response of Arabidopsis to thrips feeding on leaf discs over 1 or 2 days [23,24]. To analyze its role in long-term defense at the whole plant level, we compared the feeding damage between whole WT plants and JA-insensitive coi1-1 mutants [28] inoculated with 20 thrips at 3 weeks. The coi1-1 mutants had been completely devoured by 4 weeks after inoculation, whereas WT plants were flowering and producing siliques (Fig. 1A). These results suggest the importance of JA-regulated defense in the resistance of Arabidopsis to thrips attack.


Jasmonate-dependent plant defense restricts thrips performance and preference.

Abe H, Shimoda T, Ohnishi J, Kugimiya S, Narusaka M, Seo S, Narusaka Y, Tsuda S, Kobayashi M - BMC Plant Biol. (2009)

Function of JA in plant resistance to thrips feeding. Twenty adult females fed on 3-week-old WT plants (left) or coi1-1 mutants (right). Typical plants after 4 weeks of feeding are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2724403&req=5

Figure 1: Function of JA in plant resistance to thrips feeding. Twenty adult females fed on 3-week-old WT plants (left) or coi1-1 mutants (right). Typical plants after 4 weeks of feeding are shown.
Mentions: We recently reported the role of JA in the short-term response of Arabidopsis to thrips feeding on leaf discs over 1 or 2 days [23,24]. To analyze its role in long-term defense at the whole plant level, we compared the feeding damage between whole WT plants and JA-insensitive coi1-1 mutants [28] inoculated with 20 thrips at 3 weeks. The coi1-1 mutants had been completely devoured by 4 weeks after inoculation, whereas WT plants were flowering and producing siliques (Fig. 1A). These results suggest the importance of JA-regulated defense in the resistance of Arabidopsis to thrips attack.

Bottom Line: Application of JA to WT plants before thrips attack decreased the thrips population.Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa.Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Experimental Plant Division, RIKEN BioResource Center, Tsukuba 305-0074, Japan. ahiroshi@rtc.riken.jp

ABSTRACT

Background: The western flower thrips (Frankliniella occidentalis [Pergande]) is one of the most important insect herbivores of cultivated plants. However, no pesticide provides complete control of this species, and insecticide resistance has emerged around the world. We previously reported the important role of jasmonate (JA) in the plant's immediate response to thrips feeding by using an Arabidopsis leaf disc system. In this study, as the first step toward practical use of JA in thrips control, we analyzed the effect of JA-regulated Arabidopsis defense at the whole plant level on thrips behavior and life cycle at the population level over an extended period. We also studied the effectiveness of JA-regulated plant defense on thrips damage in Chinese cabbage (Brassica rapa subsp. pekinensis).

Results: Thrips oviposited more on Arabidopsis JA-insensitive coi1-1 mutants than on WT plants, and the population density of the following thrips generation increased on coi1-1 mutants. Moreover, thrips preferred coi1-1 mutants more than WT plants. Application of JA to WT plants before thrips attack decreased the thrips population. To analyze these important functions of JA in a brassica crop plant, we analyzed the expression of marker genes for JA response in B. rapa. Thrips feeding induced expression of these marker genes and significantly increased the JA content in B. rapa. Application of JA to B. rapa enhanced plant resistance to thrips, restricted oviposition, and reduced the population density of the following generation.

Conclusion: Our results indicate that the JA-regulated plant defense restricts thrips performance and preference, and plays an important role in the resistance of Arabidopsis and B. rapa to thrips damage.

Show MeSH
Related in: MedlinePlus