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Regulation of carboxylesterase 1 (CES1) in human adipose tissue.

Jernås M, Olsson B, Arner P, Jacobson P, Sjöström L, Walley A, Froguel P, McTernan PG, Hoffstedt J, Carlsson LM - Biochem. Biophys. Res. Commun. (2009)

Bottom Line: CES1 expression was higher in subcutaneous compared with omental adipose tissue in lean (P=0.027) and obese subjects (P=0.00036), and reduced during diet-induced weight loss (n=24, weeks 8, 16, and 18 compared to baseline, P<0.0001 for all time points).CES1 expression was higher in isolated adipocytes compared with intact adipose tissue (P=0.0018) and higher in large compared with small adipocytes (P=4.1x10(-6)).Thus, CES1 expression was linked to body fat and adipocyte fat content but not to lipolytic activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Clinical Medicine and Center for Cardiovascular and Metabolic Research, The Sahlgrenska Academy, Göteborg, Sweden. margareta.jernas@medic.gu.se

ABSTRACT
Carboxylesterase 1 (CES1) has recently been suggested to play a role in lipolysis. Our aim was to study the regulation of CES1 expression in human adipose tissue. In the SOS Sib Pair Study, CES1 expression was higher in obese compared with lean sisters (n=78 pairs, P=8.7x10(-18)) and brothers (n=12 pairs, P=0.048). CES1 expression was higher in subcutaneous compared with omental adipose tissue in lean (P=0.027) and obese subjects (P=0.00036), and reduced during diet-induced weight loss (n=24, weeks 8, 16, and 18 compared to baseline, P<0.0001 for all time points). CES1 expression was higher in isolated adipocytes compared with intact adipose tissue (P=0.0018) and higher in large compared with small adipocytes (P=4.1x10(-6)). Basal and stimulated lipolysis was not different in individuals with high, intermediate, and low expression of CES1. Thus, CES1 expression was linked to body fat and adipocyte fat content but not to lipolytic activity.

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Relation between CES1 expression and lipolysis in isolated adipocytes. Basal lipolysis (A), maximal noradrenaline-stimulated lipolysis (B), 8b-cAMP-mediated lipolysis (C), and 8b-cAMP + insulin-inhibited lipolysis (D) in samples with high, intermediate, and low CES1 mRNA levels measured by real-time PCR.
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fig2: Relation between CES1 expression and lipolysis in isolated adipocytes. Basal lipolysis (A), maximal noradrenaline-stimulated lipolysis (B), 8b-cAMP-mediated lipolysis (C), and 8b-cAMP + insulin-inhibited lipolysis (D) in samples with high, intermediate, and low CES1 mRNA levels measured by real-time PCR.

Mentions: To examine the relation between CES1 expression and lipolysis, adipocytes from different individuals were divided into tertiles based on their relative CES1 expression measured by real-time PCR. There were no differences in either basal or 8b-cAMP-mediated lipolysis between samples with low, intermediate, and high CES1 expression. Moreover, both noradrenaline-stimulated and insulin-inhibited lipolysis was similar in samples with high, intermediate, and low expression levels of CES1 mRNA (Fig. 2A–D).


Regulation of carboxylesterase 1 (CES1) in human adipose tissue.

Jernås M, Olsson B, Arner P, Jacobson P, Sjöström L, Walley A, Froguel P, McTernan PG, Hoffstedt J, Carlsson LM - Biochem. Biophys. Res. Commun. (2009)

Relation between CES1 expression and lipolysis in isolated adipocytes. Basal lipolysis (A), maximal noradrenaline-stimulated lipolysis (B), 8b-cAMP-mediated lipolysis (C), and 8b-cAMP + insulin-inhibited lipolysis (D) in samples with high, intermediate, and low CES1 mRNA levels measured by real-time PCR.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2723917&req=5

fig2: Relation between CES1 expression and lipolysis in isolated adipocytes. Basal lipolysis (A), maximal noradrenaline-stimulated lipolysis (B), 8b-cAMP-mediated lipolysis (C), and 8b-cAMP + insulin-inhibited lipolysis (D) in samples with high, intermediate, and low CES1 mRNA levels measured by real-time PCR.
Mentions: To examine the relation between CES1 expression and lipolysis, adipocytes from different individuals were divided into tertiles based on their relative CES1 expression measured by real-time PCR. There were no differences in either basal or 8b-cAMP-mediated lipolysis between samples with low, intermediate, and high CES1 expression. Moreover, both noradrenaline-stimulated and insulin-inhibited lipolysis was similar in samples with high, intermediate, and low expression levels of CES1 mRNA (Fig. 2A–D).

Bottom Line: CES1 expression was higher in subcutaneous compared with omental adipose tissue in lean (P=0.027) and obese subjects (P=0.00036), and reduced during diet-induced weight loss (n=24, weeks 8, 16, and 18 compared to baseline, P<0.0001 for all time points).CES1 expression was higher in isolated adipocytes compared with intact adipose tissue (P=0.0018) and higher in large compared with small adipocytes (P=4.1x10(-6)).Thus, CES1 expression was linked to body fat and adipocyte fat content but not to lipolytic activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Clinical Medicine and Center for Cardiovascular and Metabolic Research, The Sahlgrenska Academy, Göteborg, Sweden. margareta.jernas@medic.gu.se

ABSTRACT
Carboxylesterase 1 (CES1) has recently been suggested to play a role in lipolysis. Our aim was to study the regulation of CES1 expression in human adipose tissue. In the SOS Sib Pair Study, CES1 expression was higher in obese compared with lean sisters (n=78 pairs, P=8.7x10(-18)) and brothers (n=12 pairs, P=0.048). CES1 expression was higher in subcutaneous compared with omental adipose tissue in lean (P=0.027) and obese subjects (P=0.00036), and reduced during diet-induced weight loss (n=24, weeks 8, 16, and 18 compared to baseline, P<0.0001 for all time points). CES1 expression was higher in isolated adipocytes compared with intact adipose tissue (P=0.0018) and higher in large compared with small adipocytes (P=4.1x10(-6)). Basal and stimulated lipolysis was not different in individuals with high, intermediate, and low expression of CES1. Thus, CES1 expression was linked to body fat and adipocyte fat content but not to lipolytic activity.

Show MeSH
Related in: MedlinePlus