Limits...
Adhesion molecule-modified biomaterials for neural tissue engineering.

Rao SS, Winter JO - Front Neuroeng (2009)

Bottom Line: These tethered molecules provide cues to regenerating neurons that recapitulate the native brain environment.Improving cell adhesive potential of non-adhesive biomaterials is therefore a common goal in neural tissue engineering.Additionally, patterning of AMs for achieving specific neuronal responses is explored.

View Article: PubMed Central - PubMed

Affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University Columbus, OH, USA.

ABSTRACT
Adhesion molecules (AMs) represent one class of biomolecules that promote central nervous system regeneration. These tethered molecules provide cues to regenerating neurons that recapitulate the native brain environment. Improving cell adhesive potential of non-adhesive biomaterials is therefore a common goal in neural tissue engineering. This review discusses common AMs used in neural biomaterials and the mechanism of cell attachment to these AMs. Methods to modify materials with AMs are discussed and compared. Additionally, patterning of AMs for achieving specific neuronal responses is explored.

No MeSH data available.


Related in: MedlinePlus

Immunofluorecence of chick cortical neurons on Si wafers (5 days). (A) (PEI-Gelatin)8, (B) (PEI-gelatin)-(Chitosan-gelatin)7, (C) (PEI-Laminin)8. (Figure courtesy: Dr. Ravi Bellamkonda, Georgia Institute of Technology and Dr. Wei He, University of Tennessee.)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2723915&req=5

Figure 4: Immunofluorecence of chick cortical neurons on Si wafers (5 days). (A) (PEI-Gelatin)8, (B) (PEI-gelatin)-(Chitosan-gelatin)7, (C) (PEI-Laminin)8. (Figure courtesy: Dr. Ravi Bellamkonda, Georgia Institute of Technology and Dr. Wei He, University of Tennessee.)

Mentions: LbL coatings can be applied to tissue engineering constructs and implanted neural prostheses. Wu et al. demonstrated that LbL films comprised of hyaluronic acid (HA)/collagen promote cortical neuron adhesion on glass (Wu et al., 2007), which is normally a non-permissive substrate. Similarly, Bellamkonda et al. examined LbL films consisting of polyethylimine (PEI)/Laminin, PEI/Gelatin/Chitosan/Gelatin, and PEI/Gelatin as neural recording electrode coatings (He and Bellamkonda, 2005). Gelatin is obtained from collagen and is known to promote cell adhesion (Young et al., 2005). These coatings were shown to promote cortical neuronal adhesion and neurite extension in vitro (He and Bellamkonda, 2005) (Figure 4) and rapid reduction of early microglia activation (over a period of 4 weeks) (He et al., 2006) in vivo. This implies that LbL coatings can lower immune response over a stipulated time period.


Adhesion molecule-modified biomaterials for neural tissue engineering.

Rao SS, Winter JO - Front Neuroeng (2009)

Immunofluorecence of chick cortical neurons on Si wafers (5 days). (A) (PEI-Gelatin)8, (B) (PEI-gelatin)-(Chitosan-gelatin)7, (C) (PEI-Laminin)8. (Figure courtesy: Dr. Ravi Bellamkonda, Georgia Institute of Technology and Dr. Wei He, University of Tennessee.)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2723915&req=5

Figure 4: Immunofluorecence of chick cortical neurons on Si wafers (5 days). (A) (PEI-Gelatin)8, (B) (PEI-gelatin)-(Chitosan-gelatin)7, (C) (PEI-Laminin)8. (Figure courtesy: Dr. Ravi Bellamkonda, Georgia Institute of Technology and Dr. Wei He, University of Tennessee.)
Mentions: LbL coatings can be applied to tissue engineering constructs and implanted neural prostheses. Wu et al. demonstrated that LbL films comprised of hyaluronic acid (HA)/collagen promote cortical neuron adhesion on glass (Wu et al., 2007), which is normally a non-permissive substrate. Similarly, Bellamkonda et al. examined LbL films consisting of polyethylimine (PEI)/Laminin, PEI/Gelatin/Chitosan/Gelatin, and PEI/Gelatin as neural recording electrode coatings (He and Bellamkonda, 2005). Gelatin is obtained from collagen and is known to promote cell adhesion (Young et al., 2005). These coatings were shown to promote cortical neuronal adhesion and neurite extension in vitro (He and Bellamkonda, 2005) (Figure 4) and rapid reduction of early microglia activation (over a period of 4 weeks) (He et al., 2006) in vivo. This implies that LbL coatings can lower immune response over a stipulated time period.

Bottom Line: These tethered molecules provide cues to regenerating neurons that recapitulate the native brain environment.Improving cell adhesive potential of non-adhesive biomaterials is therefore a common goal in neural tissue engineering.Additionally, patterning of AMs for achieving specific neuronal responses is explored.

View Article: PubMed Central - PubMed

Affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University Columbus, OH, USA.

ABSTRACT
Adhesion molecules (AMs) represent one class of biomolecules that promote central nervous system regeneration. These tethered molecules provide cues to regenerating neurons that recapitulate the native brain environment. Improving cell adhesive potential of non-adhesive biomaterials is therefore a common goal in neural tissue engineering. This review discusses common AMs used in neural biomaterials and the mechanism of cell attachment to these AMs. Methods to modify materials with AMs are discussed and compared. Additionally, patterning of AMs for achieving specific neuronal responses is explored.

No MeSH data available.


Related in: MedlinePlus