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Adhesion molecule-modified biomaterials for neural tissue engineering.

Rao SS, Winter JO - Front Neuroeng (2009)

Bottom Line: These tethered molecules provide cues to regenerating neurons that recapitulate the native brain environment.Improving cell adhesive potential of non-adhesive biomaterials is therefore a common goal in neural tissue engineering.Additionally, patterning of AMs for achieving specific neuronal responses is explored.

View Article: PubMed Central - PubMed

Affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University Columbus, OH, USA.

ABSTRACT
Adhesion molecules (AMs) represent one class of biomolecules that promote central nervous system regeneration. These tethered molecules provide cues to regenerating neurons that recapitulate the native brain environment. Improving cell adhesive potential of non-adhesive biomaterials is therefore a common goal in neural tissue engineering. This review discusses common AMs used in neural biomaterials and the mechanism of cell attachment to these AMs. Methods to modify materials with AMs are discussed and compared. Additionally, patterning of AMs for achieving specific neuronal responses is explored.

No MeSH data available.


Related in: MedlinePlus

Schematic of LbL technique. Reproduced by permission of the Royal Society of Chemistry (Lutkenhaus and Hammond, 2007).
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Figure 3: Schematic of LbL technique. Reproduced by permission of the Royal Society of Chemistry (Lutkenhaus and Hammond, 2007).

Mentions: The methodology of LbL treatment (Figure 3) is relatively simple (Lutkenhaus and Hammond, 2007; Tang et al., 2006). Initial layer formation proceeds by dipping the negatively or positively charged substrate in alternating polycationic and polyanionic solutions. Between each deposition stage, the excess surface polyelectrolyte is removed by rinsing. This alternate dipping process is repeated until a desired number of bilayers with certain thickness are obtained. Factors that are critical to LbL film formation and stability include pH, polyelectrolyte loading, and ionic strength of the polyelectrolytes. The polyelectrolyte solutions can consist of the biomaterial, other polymers, drugs, or AMs. For optimal performance of AM-modified substrates the terminal layer should contain the AM of interest, permitting direct interaction with cells and tissue.


Adhesion molecule-modified biomaterials for neural tissue engineering.

Rao SS, Winter JO - Front Neuroeng (2009)

Schematic of LbL technique. Reproduced by permission of the Royal Society of Chemistry (Lutkenhaus and Hammond, 2007).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2723915&req=5

Figure 3: Schematic of LbL technique. Reproduced by permission of the Royal Society of Chemistry (Lutkenhaus and Hammond, 2007).
Mentions: The methodology of LbL treatment (Figure 3) is relatively simple (Lutkenhaus and Hammond, 2007; Tang et al., 2006). Initial layer formation proceeds by dipping the negatively or positively charged substrate in alternating polycationic and polyanionic solutions. Between each deposition stage, the excess surface polyelectrolyte is removed by rinsing. This alternate dipping process is repeated until a desired number of bilayers with certain thickness are obtained. Factors that are critical to LbL film formation and stability include pH, polyelectrolyte loading, and ionic strength of the polyelectrolytes. The polyelectrolyte solutions can consist of the biomaterial, other polymers, drugs, or AMs. For optimal performance of AM-modified substrates the terminal layer should contain the AM of interest, permitting direct interaction with cells and tissue.

Bottom Line: These tethered molecules provide cues to regenerating neurons that recapitulate the native brain environment.Improving cell adhesive potential of non-adhesive biomaterials is therefore a common goal in neural tissue engineering.Additionally, patterning of AMs for achieving specific neuronal responses is explored.

View Article: PubMed Central - PubMed

Affiliation: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University Columbus, OH, USA.

ABSTRACT
Adhesion molecules (AMs) represent one class of biomolecules that promote central nervous system regeneration. These tethered molecules provide cues to regenerating neurons that recapitulate the native brain environment. Improving cell adhesive potential of non-adhesive biomaterials is therefore a common goal in neural tissue engineering. This review discusses common AMs used in neural biomaterials and the mechanism of cell attachment to these AMs. Methods to modify materials with AMs are discussed and compared. Additionally, patterning of AMs for achieving specific neuronal responses is explored.

No MeSH data available.


Related in: MedlinePlus