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Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector.

Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, Satish Rao BS, Srinivasan KK, Priyadarsini KI, Unnikrishnan MK - Evid Based Complement Alternat Med (2007)

Bottom Line: Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells.The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls.Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576 104, Karnataka, India. cheruvaloor@yahoo.com.

ABSTRACT
Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS(*-), hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC(50) comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mug/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

No MeSH data available.


Radiation dose response curve: Influence of FRE (20 μg/ml) on micronucleus induction in Chinese hamster lung fibroblast cells (V79) administered 1 h before increasing doses of γ-irradiation. *P < 0.05 and **P < 0.01 compared to RTalone.
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Figure 6: Radiation dose response curve: Influence of FRE (20 μg/ml) on micronucleus induction in Chinese hamster lung fibroblast cells (V79) administered 1 h before increasing doses of γ-irradiation. *P < 0.05 and **P < 0.01 compared to RTalone.

Mentions: The optimum radioprotective dose, namely 20 μg/ml of FRE was selected for radiation dose response experiments. The V79 cells exposed to different doses of radiation induced a dose dependent linear increase in MN count (R = 0.99918, Fig. 6). Pretreatment of V79 cells with optimum dose of FRE for 1 h before irradiating with different doses of radiation (0.5, 1, 2, 3 and 4 Gy) produced significant (P < 0.01) decrease in percentage MNBNC compared to the respective radiation alone groups (R = 0.99201). Optimum dose of FRE was most effective in protecting (41.61%) the cells at a radiation dose of 3 Gy compared to radiation alone group, which is therapeutically relevant in clinical practice (Fig. 6).Figure 6.


Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector.

Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, Satish Rao BS, Srinivasan KK, Priyadarsini KI, Unnikrishnan MK - Evid Based Complement Alternat Med (2007)

Radiation dose response curve: Influence of FRE (20 μg/ml) on micronucleus induction in Chinese hamster lung fibroblast cells (V79) administered 1 h before increasing doses of γ-irradiation. *P < 0.05 and **P < 0.01 compared to RTalone.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722211&req=5

Figure 6: Radiation dose response curve: Influence of FRE (20 μg/ml) on micronucleus induction in Chinese hamster lung fibroblast cells (V79) administered 1 h before increasing doses of γ-irradiation. *P < 0.05 and **P < 0.01 compared to RTalone.
Mentions: The optimum radioprotective dose, namely 20 μg/ml of FRE was selected for radiation dose response experiments. The V79 cells exposed to different doses of radiation induced a dose dependent linear increase in MN count (R = 0.99918, Fig. 6). Pretreatment of V79 cells with optimum dose of FRE for 1 h before irradiating with different doses of radiation (0.5, 1, 2, 3 and 4 Gy) produced significant (P < 0.01) decrease in percentage MNBNC compared to the respective radiation alone groups (R = 0.99201). Optimum dose of FRE was most effective in protecting (41.61%) the cells at a radiation dose of 3 Gy compared to radiation alone group, which is therapeutically relevant in clinical practice (Fig. 6).Figure 6.

Bottom Line: Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells.The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls.Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576 104, Karnataka, India. cheruvaloor@yahoo.com.

ABSTRACT
Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS(*-), hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC(50) comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mug/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

No MeSH data available.