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Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector.

Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, Satish Rao BS, Srinivasan KK, Priyadarsini KI, Unnikrishnan MK - Evid Based Complement Alternat Med (2007)

Bottom Line: Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells.The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls.Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576 104, Karnataka, India. cheruvaloor@yahoo.com.

ABSTRACT
Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS(*-), hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC(50) comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mug/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

No MeSH data available.


Influence of various concentrations of FRE on micronucleus induction in Chinese hamster lung fibroblast cells (V79) administered 1 h before 2 Gy γ-irradiation. *P < 0.001 compared to Untreated; **P < 0.001 compared to Drug control (150 μg/ml); †P < 0.001 compared to Radiation treated (RT) alone; ††P < 0.05 compared to RT alone.
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Figure 5: Influence of various concentrations of FRE on micronucleus induction in Chinese hamster lung fibroblast cells (V79) administered 1 h before 2 Gy γ-irradiation. *P < 0.001 compared to Untreated; **P < 0.001 compared to Drug control (150 μg/ml); †P < 0.001 compared to Radiation treated (RT) alone; ††P < 0.05 compared to RT alone.

Mentions: The FRE by itself did not produce any clastogenic effect on V79 cells at the higher dose (150 μg/ml). A radiation-dose response (0.5–4Gy) was analyzed to optimize the best radiation dose to induce micronuclei with less cell killing. Irradiation with 2 Gy was optimal with 21.1% of micronucleated binucleate cells (MNBNC). Treatment of V79 cells with various concentration of FRE (2, 5, 10, 20, 50, 75, 100, 125 and 150 μg/ml) for 1 h before 2 Gy γ-irradiation resulted in a significant (P < 0.05 and P < 0.001) decrease in percentage of MNBNC as compared to radiation alone group. Further, a linear dose dependent decrease was observed up to a concentration of 20 μg/ml. The maximum reduction (51.42%) was observed at 20 μg/ml. However, further increase in concentration of FRE did not exhibit significant enhancement in the protection (Fig. 5).Figure 5.


Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector.

Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, Satish Rao BS, Srinivasan KK, Priyadarsini KI, Unnikrishnan MK - Evid Based Complement Alternat Med (2007)

Influence of various concentrations of FRE on micronucleus induction in Chinese hamster lung fibroblast cells (V79) administered 1 h before 2 Gy γ-irradiation. *P < 0.001 compared to Untreated; **P < 0.001 compared to Drug control (150 μg/ml); †P < 0.001 compared to Radiation treated (RT) alone; ††P < 0.05 compared to RT alone.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722211&req=5

Figure 5: Influence of various concentrations of FRE on micronucleus induction in Chinese hamster lung fibroblast cells (V79) administered 1 h before 2 Gy γ-irradiation. *P < 0.001 compared to Untreated; **P < 0.001 compared to Drug control (150 μg/ml); †P < 0.001 compared to Radiation treated (RT) alone; ††P < 0.05 compared to RT alone.
Mentions: The FRE by itself did not produce any clastogenic effect on V79 cells at the higher dose (150 μg/ml). A radiation-dose response (0.5–4Gy) was analyzed to optimize the best radiation dose to induce micronuclei with less cell killing. Irradiation with 2 Gy was optimal with 21.1% of micronucleated binucleate cells (MNBNC). Treatment of V79 cells with various concentration of FRE (2, 5, 10, 20, 50, 75, 100, 125 and 150 μg/ml) for 1 h before 2 Gy γ-irradiation resulted in a significant (P < 0.05 and P < 0.001) decrease in percentage of MNBNC as compared to radiation alone group. Further, a linear dose dependent decrease was observed up to a concentration of 20 μg/ml. The maximum reduction (51.42%) was observed at 20 μg/ml. However, further increase in concentration of FRE did not exhibit significant enhancement in the protection (Fig. 5).Figure 5.

Bottom Line: Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells.The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls.Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576 104, Karnataka, India. cheruvaloor@yahoo.com.

ABSTRACT
Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS(*-), hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC(50) comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mug/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

No MeSH data available.