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Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector.

Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, Satish Rao BS, Srinivasan KK, Priyadarsini KI, Unnikrishnan MK - Evid Based Complement Alternat Med (2007)

Bottom Line: Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells.The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls.Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576 104, Karnataka, India. cheruvaloor@yahoo.com.

ABSTRACT
Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS(*-), hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC(50) comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mug/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

No MeSH data available.


Related in: MedlinePlus

Absorption spectrum of transient species obtained by •OH attack on FRE during pulse radiolysis of aqueous solutions containing 84 μg/ml of FRE at pH 6.8 and saturated with N2O. Dose per pulse = 18.5 Gy, spectra taken 10 μs after the pulse. Inset shows •OH scavenging ability of FRE as determined by competition kinetics with KSCN, monitoring (SCN)•−2 at 500 nm. Slope of this curve gives the rate constant. A0, Absorbance of (SCN)•−2 in the absence of FRE; A, Absorbance of (SCN)•−2 in the presence of FRE.
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Figure 4: Absorption spectrum of transient species obtained by •OH attack on FRE during pulse radiolysis of aqueous solutions containing 84 μg/ml of FRE at pH 6.8 and saturated with N2O. Dose per pulse = 18.5 Gy, spectra taken 10 μs after the pulse. Inset shows •OH scavenging ability of FRE as determined by competition kinetics with KSCN, monitoring (SCN)•−2 at 500 nm. Slope of this curve gives the rate constant. A0, Absorbance of (SCN)•−2 in the absence of FRE; A, Absorbance of (SCN)•−2 in the presence of FRE.

Mentions: FRE exhibited better •OH scavenging activity (IC50 c59.08 μg/ml) than standard mannitol (IC50 of 2.95 mg/ml) under the same experimental conditions (Table 1). The reactivity, in nanosecond time scales towards •OH, by FRE was studied using nanosecond pulse radiolysis. A transient spectrum was plotted, which showed prominent peaks at 310 nm (Fig. 4). The spectra do not indicate presence of any detectable standard polyphenolic antioxidants like gallic acid, flavonoids etc., which probably indicates presence of low molecular weight phenolics. Competition kinetics method was used to determine the •OH scavenging ability of FRE (Inset of Fig. 4). FRE was found to be 2.9% as reactive as SCN•− towards •OH.Figure 4.


Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector.

Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, Satish Rao BS, Srinivasan KK, Priyadarsini KI, Unnikrishnan MK - Evid Based Complement Alternat Med (2007)

Absorption spectrum of transient species obtained by •OH attack on FRE during pulse radiolysis of aqueous solutions containing 84 μg/ml of FRE at pH 6.8 and saturated with N2O. Dose per pulse = 18.5 Gy, spectra taken 10 μs after the pulse. Inset shows •OH scavenging ability of FRE as determined by competition kinetics with KSCN, monitoring (SCN)•−2 at 500 nm. Slope of this curve gives the rate constant. A0, Absorbance of (SCN)•−2 in the absence of FRE; A, Absorbance of (SCN)•−2 in the presence of FRE.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722211&req=5

Figure 4: Absorption spectrum of transient species obtained by •OH attack on FRE during pulse radiolysis of aqueous solutions containing 84 μg/ml of FRE at pH 6.8 and saturated with N2O. Dose per pulse = 18.5 Gy, spectra taken 10 μs after the pulse. Inset shows •OH scavenging ability of FRE as determined by competition kinetics with KSCN, monitoring (SCN)•−2 at 500 nm. Slope of this curve gives the rate constant. A0, Absorbance of (SCN)•−2 in the absence of FRE; A, Absorbance of (SCN)•−2 in the presence of FRE.
Mentions: FRE exhibited better •OH scavenging activity (IC50 c59.08 μg/ml) than standard mannitol (IC50 of 2.95 mg/ml) under the same experimental conditions (Table 1). The reactivity, in nanosecond time scales towards •OH, by FRE was studied using nanosecond pulse radiolysis. A transient spectrum was plotted, which showed prominent peaks at 310 nm (Fig. 4). The spectra do not indicate presence of any detectable standard polyphenolic antioxidants like gallic acid, flavonoids etc., which probably indicates presence of low molecular weight phenolics. Competition kinetics method was used to determine the •OH scavenging ability of FRE (Inset of Fig. 4). FRE was found to be 2.9% as reactive as SCN•− towards •OH.Figure 4.

Bottom Line: Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells.The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls.Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576 104, Karnataka, India. cheruvaloor@yahoo.com.

ABSTRACT
Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS(*-), hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC(50) comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mug/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

No MeSH data available.


Related in: MedlinePlus