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Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector.

Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, Satish Rao BS, Srinivasan KK, Priyadarsini KI, Unnikrishnan MK - Evid Based Complement Alternat Med (2007)

Bottom Line: Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells.The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls.Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576 104, Karnataka, India. cheruvaloor@yahoo.com.

ABSTRACT
Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS(*-), hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC(50) comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mug/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

No MeSH data available.


Related in: MedlinePlus

Absorption-time plot showing the decay of 50 μM DPPH at 517 nm in presence of 275 μg/ml of the FRE. Inset shows linear plot for the observed decay rate constant at 517 nm as a function of different concentrations of the FRE.
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Figure 2: Absorption-time plot showing the decay of 50 μM DPPH at 517 nm in presence of 275 μg/ml of the FRE. Inset shows linear plot for the observed decay rate constant at 517 nm as a function of different concentrations of the FRE.

Mentions: FRE (IC50 5.99 μg/ml) was found to have comparable DPPH (100 μM) scavenging activity with standard ascorbic acid (ASC) under steady state conditions (IC50 2.78 μg/ml) (Table 1). In the absence of FRE, the DPPH (50 μM) signal in stopped-flow spectrophotometer did not show any decay and remained stable. However, in the presence of the extract the absorption due to the DPPH radical decayed completely in 50 s (Fig. 2). This absorption-time plot was fitted to a single exponential function to obtain observed decay rate constant (kobs), which was found to increase linearly with increasing FRE concentration. The kobs for the decay of DPPH radical in presence of FRE at 125 μg/ml was found to be 0.026 S−1 (Inset of Fig. 2).Figure 2.


Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector.

Veerapur VP, Prabhakar KR, Parihar VK, Kandadi MR, Ramakrishana S, Mishra B, Satish Rao BS, Srinivasan KK, Priyadarsini KI, Unnikrishnan MK - Evid Based Complement Alternat Med (2007)

Absorption-time plot showing the decay of 50 μM DPPH at 517 nm in presence of 275 μg/ml of the FRE. Inset shows linear plot for the observed decay rate constant at 517 nm as a function of different concentrations of the FRE.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722211&req=5

Figure 2: Absorption-time plot showing the decay of 50 μM DPPH at 517 nm in presence of 275 μg/ml of the FRE. Inset shows linear plot for the observed decay rate constant at 517 nm as a function of different concentrations of the FRE.
Mentions: FRE (IC50 5.99 μg/ml) was found to have comparable DPPH (100 μM) scavenging activity with standard ascorbic acid (ASC) under steady state conditions (IC50 2.78 μg/ml) (Table 1). In the absence of FRE, the DPPH (50 μM) signal in stopped-flow spectrophotometer did not show any decay and remained stable. However, in the presence of the extract the absorption due to the DPPH radical decayed completely in 50 s (Fig. 2). This absorption-time plot was fitted to a single exponential function to obtain observed decay rate constant (kobs), which was found to increase linearly with increasing FRE concentration. The kobs for the decay of DPPH radical in presence of FRE at 125 μg/ml was found to be 0.026 S−1 (Inset of Fig. 2).Figure 2.

Bottom Line: Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells.The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls.Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal-576 104, Karnataka, India. cheruvaloor@yahoo.com.

ABSTRACT
Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS(*-), hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC(50) comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy gamma-radiation resulted in a significant (P < 0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mug/ml of FRE. The radioprotection was found to be significant (P < 0.01) when cells were treated with optimum dose of FRE (20 mug/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy gamma-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

No MeSH data available.


Related in: MedlinePlus