Limits...
Crocus sativus L. (Saffron) extract and its active constituents (crocin and safranal) on ischemia-reperfusion in rat skeletal muscle.

Hosseinzadeh H, Modaghegh MH, Saffari Z - Evid Based Complement Alternat Med (2007)

Bottom Line: The average peak-to-peak amplitude during I/R was significantly increased in extract, crocin and safranal groups in comparison with control-ischemic group.The MDA level was declined significantly in test groups.It is concluded that saffron extract and its constituents show a protective effect against lower limb I/R in rat.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Research Center, Faculty of Pharmacy, 1365-91775, Mashhad University of Medical Sciences, Mashhad, I. R. Iran. hosseinzadehh@mums.ac.ir.

ABSTRACT
Saffron and its constituents have been shown to decrease ischemia-reperfusion (I/R) injury in kidney or brain tissues. In this study, the effects of saffron ethanolic extract and its constituents, crocin and safranal, were evaluated in skeletal muscle during I/R injury. Hind limb ischemia was induced using clamping the common femoral artery and vein. After 2 h ischemia, the clamp of the femoral vessels of animals was taken off and the animal underwent 1h reperfusion. Muscle injuries were evaluated by recording of the electromyographic (EMG) potentials and performing some biochemical analysis including thiobarbituric acid reactive substances (TBARS), total sulfhydryl (SH) groups and antioxidant capacity of muscle (using FRAP assay). The ethanolic extract of saffron (5, 20 and 80 mg kg(-1)), crocin (50, 200 and 400 mg kg(-1)), safranal (0.1, 0.25 and 0.5 ml kg(-1)) and normal saline (10 ml kg(-1)) were administered intraperitoneally 1 h prior reperfusion. The average peak-to-peak amplitude during I/R was significantly increased in extract, crocin and safranal groups in comparison with control-ischemic group. Following saffron, crocin and safranal administration, the total SH contents and antioxidant capacity were elevated in muscle flap. The MDA level was declined significantly in test groups. It is concluded that saffron extract and its constituents show a protective effect against lower limb I/R in rat.

No MeSH data available.


Related in: MedlinePlus

Effect of ethanolic saffron extract on lipid peroxidation following muscle ischemia reperfusion injury. MDA levels were measured in 10% homogenates of muscle samples from rats subjected to 120 min of ischemia and 60 min of reperfusion. All drugs were administrated intraperitoneally 60 min prior to reperfusion. Values are mean ± SEM (n = 8). *P <0.05, ***P <0.001 as compared with vehicle (normal saline) treated animals (One-way ANOVA followed by Tukey–Kramer test).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2722204&req=5

Figure 1: Effect of ethanolic saffron extract on lipid peroxidation following muscle ischemia reperfusion injury. MDA levels were measured in 10% homogenates of muscle samples from rats subjected to 120 min of ischemia and 60 min of reperfusion. All drugs were administrated intraperitoneally 60 min prior to reperfusion. Values are mean ± SEM (n = 8). *P <0.05, ***P <0.001 as compared with vehicle (normal saline) treated animals (One-way ANOVA followed by Tukey–Kramer test).

Mentions: There was an increase in the MDA levels following I/R as compared with sham-operated animals (Fig. 1). The saffron extract, crocin and safranal pretreatment resulted in a significant reduction in the free radical-mediated lipid peroxidation as indicated by a decrease in the MDA levels, at various dose levels (Figs 1, 2 and 3). In safranal-pretreated groups, a reduction in TBARS levels was very prominent in the higher doses.Figure 1.


Crocus sativus L. (Saffron) extract and its active constituents (crocin and safranal) on ischemia-reperfusion in rat skeletal muscle.

Hosseinzadeh H, Modaghegh MH, Saffari Z - Evid Based Complement Alternat Med (2007)

Effect of ethanolic saffron extract on lipid peroxidation following muscle ischemia reperfusion injury. MDA levels were measured in 10% homogenates of muscle samples from rats subjected to 120 min of ischemia and 60 min of reperfusion. All drugs were administrated intraperitoneally 60 min prior to reperfusion. Values are mean ± SEM (n = 8). *P <0.05, ***P <0.001 as compared with vehicle (normal saline) treated animals (One-way ANOVA followed by Tukey–Kramer test).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722204&req=5

Figure 1: Effect of ethanolic saffron extract on lipid peroxidation following muscle ischemia reperfusion injury. MDA levels were measured in 10% homogenates of muscle samples from rats subjected to 120 min of ischemia and 60 min of reperfusion. All drugs were administrated intraperitoneally 60 min prior to reperfusion. Values are mean ± SEM (n = 8). *P <0.05, ***P <0.001 as compared with vehicle (normal saline) treated animals (One-way ANOVA followed by Tukey–Kramer test).
Mentions: There was an increase in the MDA levels following I/R as compared with sham-operated animals (Fig. 1). The saffron extract, crocin and safranal pretreatment resulted in a significant reduction in the free radical-mediated lipid peroxidation as indicated by a decrease in the MDA levels, at various dose levels (Figs 1, 2 and 3). In safranal-pretreated groups, a reduction in TBARS levels was very prominent in the higher doses.Figure 1.

Bottom Line: The average peak-to-peak amplitude during I/R was significantly increased in extract, crocin and safranal groups in comparison with control-ischemic group.The MDA level was declined significantly in test groups.It is concluded that saffron extract and its constituents show a protective effect against lower limb I/R in rat.

View Article: PubMed Central - PubMed

Affiliation: Pharmaceutical Research Center, Faculty of Pharmacy, 1365-91775, Mashhad University of Medical Sciences, Mashhad, I. R. Iran. hosseinzadehh@mums.ac.ir.

ABSTRACT
Saffron and its constituents have been shown to decrease ischemia-reperfusion (I/R) injury in kidney or brain tissues. In this study, the effects of saffron ethanolic extract and its constituents, crocin and safranal, were evaluated in skeletal muscle during I/R injury. Hind limb ischemia was induced using clamping the common femoral artery and vein. After 2 h ischemia, the clamp of the femoral vessels of animals was taken off and the animal underwent 1h reperfusion. Muscle injuries were evaluated by recording of the electromyographic (EMG) potentials and performing some biochemical analysis including thiobarbituric acid reactive substances (TBARS), total sulfhydryl (SH) groups and antioxidant capacity of muscle (using FRAP assay). The ethanolic extract of saffron (5, 20 and 80 mg kg(-1)), crocin (50, 200 and 400 mg kg(-1)), safranal (0.1, 0.25 and 0.5 ml kg(-1)) and normal saline (10 ml kg(-1)) were administered intraperitoneally 1 h prior reperfusion. The average peak-to-peak amplitude during I/R was significantly increased in extract, crocin and safranal groups in comparison with control-ischemic group. Following saffron, crocin and safranal administration, the total SH contents and antioxidant capacity were elevated in muscle flap. The MDA level was declined significantly in test groups. It is concluded that saffron extract and its constituents show a protective effect against lower limb I/R in rat.

No MeSH data available.


Related in: MedlinePlus