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Phytic Acid Exposure Alters AflatoxinB1-induced Reproductive and Oxidative Toxicity in Albino Rats (Rattus norvegicus).

Abu El-Saad AS, Mahmoud HM - Evid Based Complement Alternat Med (2007)

Bottom Line: The effects of AFB1 treatment on the absolute and relative weight of testis as well as its histopathologic effect on the testis and the possibility of amelioration by IP6 treatment were evaluated.A decrease in sex hormone levels, an increase in testicular lipid peroxidation product levels and a significant decrease in testicular glutathione content, catalase and total peroxidase and superoxide dismutase activities were recorded.It is concluded that the reduction in the toxicity of free radicals by phytic acid might be responsible for the protective influence observed.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Faculty of Science, American University in Cairo, 11511, Egypt or 113 Kasr el Aini Street, PO Box 2511, Cairo 11511, Egypt. mhmada@aucegypt.edu.

ABSTRACT
The increased use of feed in Egypt's aquaculture and animal industries raises concerns about the possible presence of mycotoxins in feedstuffs. The use of alternative medicine, such as botanicals and nutritional supplements, has become popular with inflammatory cases. The present study aimed to testify the role played by phytic acid (IP6) in enhancing the reproductive and oxidative toxicity induced in aflatoxinB1 (AFB1) treated white male albino rats (Rattus norvegicus) throughout treatment and withdrawal periods. One hundred and twenty white male albino rats were grouped into four groups. Group 1, was injected with 300 mug kg(-1) body wt of AFB1 once every 3 days for 15 days and left uninjected for another 15 days to study the withdrawal effect. Group 2, was injected with 300 mug kg(-1) body wt of AFB1 once every 3 days for 15 days and treated simultaneously with IP6 daily for another 15 days. Group 3, was treated daily with IP6 (40 mg kg(-1) body wt) for 15 days and with no treatment for other 15 days. Group 4, injected with equivalent volume of sterile phosphate buffer saline solution as a control group. Sera were taken at the experimental intervals and assayed for testosterone hormone, follicular-stimulating hormone (FSH) and luteinizing hormone (LH) to determine the toxicological impact of AFB1 and the possibility of amelioration by phytic acid on the reproductive performance of the studied animal. The effects of AFB1 treatment on the absolute and relative weight of testis as well as its histopathologic effect on the testis and the possibility of amelioration by IP6 treatment were evaluated. The activities of enzymatic and non-enzymatic anti-oxidants, in addition to lipid peroxidation were measured in the testis' homogenate of AFB1-treated rats. A decrease in sex hormone levels, an increase in testicular lipid peroxidation product levels and a significant decrease in testicular glutathione content, catalase and total peroxidase and superoxide dismutase activities were recorded. The histopathologic alterations revealed a degeneration and highly mitotic division within the spermatogenic nuclei, in addition to some karyomegaly and nuclear pyknosis. It is concluded that the reduction in the toxicity of free radicals by phytic acid might be responsible for the protective influence observed.

No MeSH data available.


Related in: MedlinePlus

A photomicrograph shows a marked regenerative effect after 1 week post-treatment with phytic acid. H & E × 10.
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Figure 6: A photomicrograph shows a marked regenerative effect after 1 week post-treatment with phytic acid. H & E × 10.

Mentions: Control sections of testis revealed that the seminiferous tubules consist of several layers of epithelial cells (Fig. 3). These spermatogonia cells are cuboidal cells, having clear cytoplasm and rounded nuclei, which may show division. Sertoli cells are large epithelial cells having vesicular nucleus with large nucleolus. Next to the spermatogonia cells is a zone of spermatocytes, the nuclei of which are usually in mitotic division. A large number of small cells, the spermatids, are seen external to spermatocytes. The spermatids become developed further into spermatozoa. The latter usually lie in groups with their heads projecting between the deeper cells, and are connected with one of the sertoli cells of the lining epithelium. By contrast, sections treated with AFB1 showed degenerative and highly mitotic division after 3 days of injection. Also, spermatozoa were seemed to disappear in association with karyomegaly in some nuclei of the spermatogonic cells, while others show pyknotic. Meanwhile, at 3rd day post-AFB1 injection, H and E sections of testis show degeneration in the seminiferous tubules with complete absence of spermatozoa, in addition, some spermatogonic nuclei show karyomegaly and high incidence of mitotic divisions (Figs 4 and 5). On the other hand, treatment with phytic acid for 1 week shows a marked regenerative effect upon the histopathologic features of the seminiferous tubules (Fig. 6). A reduction in spermatids number within the lumen of seminiferous tubules, a marked mitotic divisions and pyknosis of some spermatogonic nuclei were observed in the section of testis of AFB1-injected group after 2 weeks of injection (Figs 7 and 8). Testis sections of rats treated with AFB1–phytic acid mixture show regenerative features after 2 weeks of treatments, in spite of the absence of spermatids (Figs 9 and 10).Figure 3.


Phytic Acid Exposure Alters AflatoxinB1-induced Reproductive and Oxidative Toxicity in Albino Rats (Rattus norvegicus).

Abu El-Saad AS, Mahmoud HM - Evid Based Complement Alternat Med (2007)

A photomicrograph shows a marked regenerative effect after 1 week post-treatment with phytic acid. H & E × 10.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722202&req=5

Figure 6: A photomicrograph shows a marked regenerative effect after 1 week post-treatment with phytic acid. H & E × 10.
Mentions: Control sections of testis revealed that the seminiferous tubules consist of several layers of epithelial cells (Fig. 3). These spermatogonia cells are cuboidal cells, having clear cytoplasm and rounded nuclei, which may show division. Sertoli cells are large epithelial cells having vesicular nucleus with large nucleolus. Next to the spermatogonia cells is a zone of spermatocytes, the nuclei of which are usually in mitotic division. A large number of small cells, the spermatids, are seen external to spermatocytes. The spermatids become developed further into spermatozoa. The latter usually lie in groups with their heads projecting between the deeper cells, and are connected with one of the sertoli cells of the lining epithelium. By contrast, sections treated with AFB1 showed degenerative and highly mitotic division after 3 days of injection. Also, spermatozoa were seemed to disappear in association with karyomegaly in some nuclei of the spermatogonic cells, while others show pyknotic. Meanwhile, at 3rd day post-AFB1 injection, H and E sections of testis show degeneration in the seminiferous tubules with complete absence of spermatozoa, in addition, some spermatogonic nuclei show karyomegaly and high incidence of mitotic divisions (Figs 4 and 5). On the other hand, treatment with phytic acid for 1 week shows a marked regenerative effect upon the histopathologic features of the seminiferous tubules (Fig. 6). A reduction in spermatids number within the lumen of seminiferous tubules, a marked mitotic divisions and pyknosis of some spermatogonic nuclei were observed in the section of testis of AFB1-injected group after 2 weeks of injection (Figs 7 and 8). Testis sections of rats treated with AFB1–phytic acid mixture show regenerative features after 2 weeks of treatments, in spite of the absence of spermatids (Figs 9 and 10).Figure 3.

Bottom Line: The effects of AFB1 treatment on the absolute and relative weight of testis as well as its histopathologic effect on the testis and the possibility of amelioration by IP6 treatment were evaluated.A decrease in sex hormone levels, an increase in testicular lipid peroxidation product levels and a significant decrease in testicular glutathione content, catalase and total peroxidase and superoxide dismutase activities were recorded.It is concluded that the reduction in the toxicity of free radicals by phytic acid might be responsible for the protective influence observed.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Faculty of Science, American University in Cairo, 11511, Egypt or 113 Kasr el Aini Street, PO Box 2511, Cairo 11511, Egypt. mhmada@aucegypt.edu.

ABSTRACT
The increased use of feed in Egypt's aquaculture and animal industries raises concerns about the possible presence of mycotoxins in feedstuffs. The use of alternative medicine, such as botanicals and nutritional supplements, has become popular with inflammatory cases. The present study aimed to testify the role played by phytic acid (IP6) in enhancing the reproductive and oxidative toxicity induced in aflatoxinB1 (AFB1) treated white male albino rats (Rattus norvegicus) throughout treatment and withdrawal periods. One hundred and twenty white male albino rats were grouped into four groups. Group 1, was injected with 300 mug kg(-1) body wt of AFB1 once every 3 days for 15 days and left uninjected for another 15 days to study the withdrawal effect. Group 2, was injected with 300 mug kg(-1) body wt of AFB1 once every 3 days for 15 days and treated simultaneously with IP6 daily for another 15 days. Group 3, was treated daily with IP6 (40 mg kg(-1) body wt) for 15 days and with no treatment for other 15 days. Group 4, injected with equivalent volume of sterile phosphate buffer saline solution as a control group. Sera were taken at the experimental intervals and assayed for testosterone hormone, follicular-stimulating hormone (FSH) and luteinizing hormone (LH) to determine the toxicological impact of AFB1 and the possibility of amelioration by phytic acid on the reproductive performance of the studied animal. The effects of AFB1 treatment on the absolute and relative weight of testis as well as its histopathologic effect on the testis and the possibility of amelioration by IP6 treatment were evaluated. The activities of enzymatic and non-enzymatic anti-oxidants, in addition to lipid peroxidation were measured in the testis' homogenate of AFB1-treated rats. A decrease in sex hormone levels, an increase in testicular lipid peroxidation product levels and a significant decrease in testicular glutathione content, catalase and total peroxidase and superoxide dismutase activities were recorded. The histopathologic alterations revealed a degeneration and highly mitotic division within the spermatogenic nuclei, in addition to some karyomegaly and nuclear pyknosis. It is concluded that the reduction in the toxicity of free radicals by phytic acid might be responsible for the protective influence observed.

No MeSH data available.


Related in: MedlinePlus