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Improvement of Experimentally Induced Hepatic and Renal Disorders in Rats using Lactic Acid Bacteria-fermented Soybean Extract (BiofermenticsTM).

Shin R, Suzuki M, Mizutani T, Susa N - Evid Based Complement Alternat Med (2007)

Bottom Line: In rat, hepatitis induced by feeding of deoxycholic acid (DCA, 0.5 wt/wt, n = 6) or intraperitoneal injection of d-galactosamine (GMN, 500 mg/body wt, n = 6), the increase in serum AST (aspartate aminotransferase) and ALT (alanine aminotransferase) levels were inhibited significantly (P < 0.05) by feeding a diet containing 5% dried BF.Moreover, the BF-administered rat group showed lower concentrations of blood urea nitrogen and a larger amount of urine as compared with values in the control group.These results suggest that BF may play a role in hepatic and renal disorders, and may be useful for maintaining health in humans as well.

View Article: PubMed Central - PubMed

Affiliation: Central Institute for Health Science, A.L.A. Corporation 40-14 Kitamachi, Seya-ku, Yokohama-city, Kanagawa, 246-0002 Japan. rshin@ciala.co.jp.

ABSTRACT
The effects of lactic acid bacteria-fermented soybean extract (Biofermentics; BF) on experimental models of hepatic and renal disorders were investigated in vivo and in vitro. In rat, hepatitis induced by feeding of deoxycholic acid (DCA, 0.5 wt/wt, n = 6) or intraperitoneal injection of d-galactosamine (GMN, 500 mg/body wt, n = 6), the increase in serum AST (aspartate aminotransferase) and ALT (alanine aminotransferase) levels were inhibited significantly (P < 0.05) by feeding a diet containing 5% dried BF. Moreover, the BF-administered rat group showed lower concentrations of blood urea nitrogen and a larger amount of urine as compared with values in the control group. Pretreatment of primary cell cultures of rat hepatic and renal cells with BF prior to exposure to dichromate (K(2)Cr(2)O(7)) resulted in a marked decrease of dichromate-induced cytotoxicity as evaluated by the leakage of lactate dehydrogenase The levels of dichromate-induced lipid peroxidation, as monitored by malondialdehyde formation, were also reduced by pretreatment of hepatocytes with BF. These results suggest that BF may play a role in hepatic and renal disorders, and may be useful for maintaining health in humans as well.

No MeSH data available.


Related in: MedlinePlus

Effects of BF treatment on dichromate-induced cytotoxicity in primary cultures of rat hepatocytes. Hepatocytes were treated with BF alone (open square) or with a combination of BF and dichromate (1 mM) (filled square) for 8 h at 37°C in SGM. The effect of BF was evaluated as a percent of decreased LDH leakage against the total LDH from control hepatocytes without any treatment. Bars indicate mean ± SD (n = 4). P < 0.05 of superscript a as compared with values of superscript b and c. BF, Biofermentics™ (lactic acid bacteria-fermented soybean extract). LDH, lactate dehydrogenase.
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Figure 3: Effects of BF treatment on dichromate-induced cytotoxicity in primary cultures of rat hepatocytes. Hepatocytes were treated with BF alone (open square) or with a combination of BF and dichromate (1 mM) (filled square) for 8 h at 37°C in SGM. The effect of BF was evaluated as a percent of decreased LDH leakage against the total LDH from control hepatocytes without any treatment. Bars indicate mean ± SD (n = 4). P < 0.05 of superscript a as compared with values of superscript b and c. BF, Biofermentics™ (lactic acid bacteria-fermented soybean extract). LDH, lactate dehydrogenase.

Mentions: As shown in Fig. 3, when the hepatocytes were treated with 1 mM dichromate, they were damaged and leaked LDH at a level of 18% of total LDH in normal cells. However, when they were treated with dichromate and BF at the same time, the LDH leakage was significantly inhibited to a level of 7.0% of untreated control cells, indicating that BF treatment had a protective effect against cytotoxicity caused by dichromate treatment. No adverse effects were observed with BF treatment in the range of concentrations employed.Figure 3.


Improvement of Experimentally Induced Hepatic and Renal Disorders in Rats using Lactic Acid Bacteria-fermented Soybean Extract (BiofermenticsTM).

Shin R, Suzuki M, Mizutani T, Susa N - Evid Based Complement Alternat Med (2007)

Effects of BF treatment on dichromate-induced cytotoxicity in primary cultures of rat hepatocytes. Hepatocytes were treated with BF alone (open square) or with a combination of BF and dichromate (1 mM) (filled square) for 8 h at 37°C in SGM. The effect of BF was evaluated as a percent of decreased LDH leakage against the total LDH from control hepatocytes without any treatment. Bars indicate mean ± SD (n = 4). P < 0.05 of superscript a as compared with values of superscript b and c. BF, Biofermentics™ (lactic acid bacteria-fermented soybean extract). LDH, lactate dehydrogenase.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722200&req=5

Figure 3: Effects of BF treatment on dichromate-induced cytotoxicity in primary cultures of rat hepatocytes. Hepatocytes were treated with BF alone (open square) or with a combination of BF and dichromate (1 mM) (filled square) for 8 h at 37°C in SGM. The effect of BF was evaluated as a percent of decreased LDH leakage against the total LDH from control hepatocytes without any treatment. Bars indicate mean ± SD (n = 4). P < 0.05 of superscript a as compared with values of superscript b and c. BF, Biofermentics™ (lactic acid bacteria-fermented soybean extract). LDH, lactate dehydrogenase.
Mentions: As shown in Fig. 3, when the hepatocytes were treated with 1 mM dichromate, they were damaged and leaked LDH at a level of 18% of total LDH in normal cells. However, when they were treated with dichromate and BF at the same time, the LDH leakage was significantly inhibited to a level of 7.0% of untreated control cells, indicating that BF treatment had a protective effect against cytotoxicity caused by dichromate treatment. No adverse effects were observed with BF treatment in the range of concentrations employed.Figure 3.

Bottom Line: In rat, hepatitis induced by feeding of deoxycholic acid (DCA, 0.5 wt/wt, n = 6) or intraperitoneal injection of d-galactosamine (GMN, 500 mg/body wt, n = 6), the increase in serum AST (aspartate aminotransferase) and ALT (alanine aminotransferase) levels were inhibited significantly (P < 0.05) by feeding a diet containing 5% dried BF.Moreover, the BF-administered rat group showed lower concentrations of blood urea nitrogen and a larger amount of urine as compared with values in the control group.These results suggest that BF may play a role in hepatic and renal disorders, and may be useful for maintaining health in humans as well.

View Article: PubMed Central - PubMed

Affiliation: Central Institute for Health Science, A.L.A. Corporation 40-14 Kitamachi, Seya-ku, Yokohama-city, Kanagawa, 246-0002 Japan. rshin@ciala.co.jp.

ABSTRACT
The effects of lactic acid bacteria-fermented soybean extract (Biofermentics; BF) on experimental models of hepatic and renal disorders were investigated in vivo and in vitro. In rat, hepatitis induced by feeding of deoxycholic acid (DCA, 0.5 wt/wt, n = 6) or intraperitoneal injection of d-galactosamine (GMN, 500 mg/body wt, n = 6), the increase in serum AST (aspartate aminotransferase) and ALT (alanine aminotransferase) levels were inhibited significantly (P < 0.05) by feeding a diet containing 5% dried BF. Moreover, the BF-administered rat group showed lower concentrations of blood urea nitrogen and a larger amount of urine as compared with values in the control group. Pretreatment of primary cell cultures of rat hepatic and renal cells with BF prior to exposure to dichromate (K(2)Cr(2)O(7)) resulted in a marked decrease of dichromate-induced cytotoxicity as evaluated by the leakage of lactate dehydrogenase The levels of dichromate-induced lipid peroxidation, as monitored by malondialdehyde formation, were also reduced by pretreatment of hepatocytes with BF. These results suggest that BF may play a role in hepatic and renal disorders, and may be useful for maintaining health in humans as well.

No MeSH data available.


Related in: MedlinePlus