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Novel Diagnosis of Lyme Disease: Potential for CAM Intervention.

Vojdani A, Hebroni F, Raphael Y, Erde J, Raxlen B - Evid Based Complement Alternat Med (2007)

Bottom Line: Lyme disease (LD) is the most common tick-borne disease in the northern hemisphere, producing a wide range of disabling effects on multiple human targets, including the skin, the nervous system, the joints and the heart.The goal of this article is 4-fold: (i) to detail LD infection and pathology, (ii) to review prevalent diagnostic methods, emphasizing inherent problems, (iii) to introduce the usage of in vivo induced antigen technology (IVIAT) in clinical diagnostics and (iv) to underscore the relevance of a novel comprehensive LD diagnostic approach to practitioners of Complementary and Alternative Medicine (CAM).Utilization of this analytical method will increase the accuracy of the diagnostic process and abridge the time to treatment, with antibiotics, herbal medicines and nutritional supplements, resulting in improved quality of care and disease prognosis.

View Article: PubMed Central - PubMed

Affiliation: Immunosciences Lab., Inc., 8693 Wilshire Blvd., Suite 200, Beverly Hills, CA 90211, USA. drari@msn.com.

ABSTRACT
Lyme disease (LD) is the most common tick-borne disease in the northern hemisphere, producing a wide range of disabling effects on multiple human targets, including the skin, the nervous system, the joints and the heart. Insufficient clinical diagnostic methods, the necessity for prompt antibiotic treatment along with the pervasive nature of infection impel the development and establishment of new clinical diagnostic tools with increased accuracy, sensitivity and specificity. The goal of this article is 4-fold: (i) to detail LD infection and pathology, (ii) to review prevalent diagnostic methods, emphasizing inherent problems, (iii) to introduce the usage of in vivo induced antigen technology (IVIAT) in clinical diagnostics and (iv) to underscore the relevance of a novel comprehensive LD diagnostic approach to practitioners of Complementary and Alternative Medicine (CAM). Utilization of this analytical method will increase the accuracy of the diagnostic process and abridge the time to treatment, with antibiotics, herbal medicines and nutritional supplements, resulting in improved quality of care and disease prognosis.

No MeSH data available.


Related in: MedlinePlus

IgG + IgM measured against 16 different peptides and antigens from Borrelia, cross-reactive microorganisms (Babesia, Ehrlichia, Bartonella, T. palidum) and target tissue antigens such as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG) and collagen. Index was calculated based on ELISA optical densities of each specimen obtained at 405 nm divided by the OD of normal human serum tested against the same antigen or peptide simultaneously. Note that each patient has a different pattern of antibody reactivity against the sixteen different tested antigens or peptides.
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Figure 2: IgG + IgM measured against 16 different peptides and antigens from Borrelia, cross-reactive microorganisms (Babesia, Ehrlichia, Bartonella, T. palidum) and target tissue antigens such as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG) and collagen. Index was calculated based on ELISA optical densities of each specimen obtained at 405 nm divided by the OD of normal human serum tested against the same antigen or peptide simultaneously. Note that each patient has a different pattern of antibody reactivity against the sixteen different tested antigens or peptides.

Mentions: LD is a complex and devastating disorder requiring efficient means of diagnosis in order to begin treatment at the onset of infection. We have outlined the method of transmission and the mechanisms of infection and maintenance, both of which provide critical information for developing new diagnostic techniques. During the various life cycles of Borrelia in the body, the expression of different antigens is tightly regulated such that the spirochete may disseminate and survive for long periods of time. We have shown that the variety of antigens, identified through IVIAT (43), is integral to accurately identify infection and indicate the stage of infection. Information gauged from clinical diagnostic methods assessing these two components, most easily implemented into an ELISA method, provides an infection profile from which an appropriate medical treatment may be determined. In addition to IVIAT and its use in multi-peptide ELISA, work is being done to develop a method to detect immune response to Ixodes proteins (32–34, 93, 94). Using this novel technology for the diagnosis of LD, examples of four patients with signs and symptoms of LD are depicted in Fig. 2. Each specimen was tested for IgG and IgM antibodies simultaneously against B. burgdorferi lysate and peptides from outer surface protein-A (OspA) and OspC, OspE, LFA, immunodominant peptides (C2 + C6), VMP, B. b. sensu stricto, B. garinii, B. afzelii, Babesia, Ehrlichia, Bartonella, Treponema palidum, as well as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG) and collagen as tissue antigens.Figure 2.


Novel Diagnosis of Lyme Disease: Potential for CAM Intervention.

Vojdani A, Hebroni F, Raphael Y, Erde J, Raxlen B - Evid Based Complement Alternat Med (2007)

IgG + IgM measured against 16 different peptides and antigens from Borrelia, cross-reactive microorganisms (Babesia, Ehrlichia, Bartonella, T. palidum) and target tissue antigens such as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG) and collagen. Index was calculated based on ELISA optical densities of each specimen obtained at 405 nm divided by the OD of normal human serum tested against the same antigen or peptide simultaneously. Note that each patient has a different pattern of antibody reactivity against the sixteen different tested antigens or peptides.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722197&req=5

Figure 2: IgG + IgM measured against 16 different peptides and antigens from Borrelia, cross-reactive microorganisms (Babesia, Ehrlichia, Bartonella, T. palidum) and target tissue antigens such as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG) and collagen. Index was calculated based on ELISA optical densities of each specimen obtained at 405 nm divided by the OD of normal human serum tested against the same antigen or peptide simultaneously. Note that each patient has a different pattern of antibody reactivity against the sixteen different tested antigens or peptides.
Mentions: LD is a complex and devastating disorder requiring efficient means of diagnosis in order to begin treatment at the onset of infection. We have outlined the method of transmission and the mechanisms of infection and maintenance, both of which provide critical information for developing new diagnostic techniques. During the various life cycles of Borrelia in the body, the expression of different antigens is tightly regulated such that the spirochete may disseminate and survive for long periods of time. We have shown that the variety of antigens, identified through IVIAT (43), is integral to accurately identify infection and indicate the stage of infection. Information gauged from clinical diagnostic methods assessing these two components, most easily implemented into an ELISA method, provides an infection profile from which an appropriate medical treatment may be determined. In addition to IVIAT and its use in multi-peptide ELISA, work is being done to develop a method to detect immune response to Ixodes proteins (32–34, 93, 94). Using this novel technology for the diagnosis of LD, examples of four patients with signs and symptoms of LD are depicted in Fig. 2. Each specimen was tested for IgG and IgM antibodies simultaneously against B. burgdorferi lysate and peptides from outer surface protein-A (OspA) and OspC, OspE, LFA, immunodominant peptides (C2 + C6), VMP, B. b. sensu stricto, B. garinii, B. afzelii, Babesia, Ehrlichia, Bartonella, Treponema palidum, as well as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG) and collagen as tissue antigens.Figure 2.

Bottom Line: Lyme disease (LD) is the most common tick-borne disease in the northern hemisphere, producing a wide range of disabling effects on multiple human targets, including the skin, the nervous system, the joints and the heart.The goal of this article is 4-fold: (i) to detail LD infection and pathology, (ii) to review prevalent diagnostic methods, emphasizing inherent problems, (iii) to introduce the usage of in vivo induced antigen technology (IVIAT) in clinical diagnostics and (iv) to underscore the relevance of a novel comprehensive LD diagnostic approach to practitioners of Complementary and Alternative Medicine (CAM).Utilization of this analytical method will increase the accuracy of the diagnostic process and abridge the time to treatment, with antibiotics, herbal medicines and nutritional supplements, resulting in improved quality of care and disease prognosis.

View Article: PubMed Central - PubMed

Affiliation: Immunosciences Lab., Inc., 8693 Wilshire Blvd., Suite 200, Beverly Hills, CA 90211, USA. drari@msn.com.

ABSTRACT
Lyme disease (LD) is the most common tick-borne disease in the northern hemisphere, producing a wide range of disabling effects on multiple human targets, including the skin, the nervous system, the joints and the heart. Insufficient clinical diagnostic methods, the necessity for prompt antibiotic treatment along with the pervasive nature of infection impel the development and establishment of new clinical diagnostic tools with increased accuracy, sensitivity and specificity. The goal of this article is 4-fold: (i) to detail LD infection and pathology, (ii) to review prevalent diagnostic methods, emphasizing inherent problems, (iii) to introduce the usage of in vivo induced antigen technology (IVIAT) in clinical diagnostics and (iv) to underscore the relevance of a novel comprehensive LD diagnostic approach to practitioners of Complementary and Alternative Medicine (CAM). Utilization of this analytical method will increase the accuracy of the diagnostic process and abridge the time to treatment, with antibiotics, herbal medicines and nutritional supplements, resulting in improved quality of care and disease prognosis.

No MeSH data available.


Related in: MedlinePlus