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Heterelogous expression of plant genes.

Yesilirmak F, Sayers Z - Int J Plant Genomics (2009)

Bottom Line: Heterologous expression allows the production of plant proteins in an organism which is simpler than the natural source.This technology is widely used for large-scale purification of plant proteins from microorganisms for biochemical and biophysical analyses.Additionally expression in well-defined model organisms provides insights into the functions of proteins in complex pathways.

View Article: PubMed Central - PubMed

Affiliation: Sabanci University, Orhanli, Tuzla, Istanbul, Turkey. filizy@sabanciuniv.edu <filizy@sabanciuniv.edu>

ABSTRACT
Heterologous expression allows the production of plant proteins in an organism which is simpler than the natural source. This technology is widely used for large-scale purification of plant proteins from microorganisms for biochemical and biophysical analyses. Additionally expression in well-defined model organisms provides insights into the functions of proteins in complex pathways. The present review gives an overview of recombinant plant protein production methods using bacteria, yeast, insect cells, and Xenopus laevis oocytes and discusses the advantages of each system for functional studies and protein characterization.

No MeSH data available.


Flow chart for heterologous expression.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig1: Flow chart for heterologous expression.

Mentions: Basic principles of heterologous cloning and expression are summarized in Figure 1. Major parameters that affect choices at different stages are also indicated. The choice of the expression system and vector is a critical step in this procedure and, as indicated, advantages and disadvantages of several factors have to be considered. Expression systems are selected depending on whether the purpose of study is production of large quantities of protein or investigation of functional features of the cloned protein. The physicochemical properties of the investigated protein also play a role in this choice. A general review of frequently used expression systems is provided by Yin et al. [3].


Heterelogous expression of plant genes.

Yesilirmak F, Sayers Z - Int J Plant Genomics (2009)

Flow chart for heterologous expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2722100&req=5

fig1: Flow chart for heterologous expression.
Mentions: Basic principles of heterologous cloning and expression are summarized in Figure 1. Major parameters that affect choices at different stages are also indicated. The choice of the expression system and vector is a critical step in this procedure and, as indicated, advantages and disadvantages of several factors have to be considered. Expression systems are selected depending on whether the purpose of study is production of large quantities of protein or investigation of functional features of the cloned protein. The physicochemical properties of the investigated protein also play a role in this choice. A general review of frequently used expression systems is provided by Yin et al. [3].

Bottom Line: Heterologous expression allows the production of plant proteins in an organism which is simpler than the natural source.This technology is widely used for large-scale purification of plant proteins from microorganisms for biochemical and biophysical analyses.Additionally expression in well-defined model organisms provides insights into the functions of proteins in complex pathways.

View Article: PubMed Central - PubMed

Affiliation: Sabanci University, Orhanli, Tuzla, Istanbul, Turkey. filizy@sabanciuniv.edu <filizy@sabanciuniv.edu>

ABSTRACT
Heterologous expression allows the production of plant proteins in an organism which is simpler than the natural source. This technology is widely used for large-scale purification of plant proteins from microorganisms for biochemical and biophysical analyses. Additionally expression in well-defined model organisms provides insights into the functions of proteins in complex pathways. The present review gives an overview of recombinant plant protein production methods using bacteria, yeast, insect cells, and Xenopus laevis oocytes and discusses the advantages of each system for functional studies and protein characterization.

No MeSH data available.