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50 Hz extremely low frequency electromagnetic fields enhance protein carbonyl groups content in cancer cells: effects on proteasomal systems.

Eleuteri AM, Amici M, Bonfili L, Cecarini V, Cuccioloni M, Grimaldi S, Giuliani L, Angeletti M, Fioretti E - J. Biomed. Biotechnol. (2009)

Bottom Line: This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins.Caco 2 cells were exposed, for 24-72 hours, to 1 mT, 50 Hz electromagnetic fields.The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology M.C.A., University of Camerino, 62032 Camerino (MC), Italy. annamaria.eleuteri@unicam.it

ABSTRACT
Electromagnetic fields are an assessed cause of prolonging free radicals lifespan. This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins. Caco 2 cells were exposed, for 24-72 hours, to 1 mT, 50 Hz electromagnetic fields. The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected. Exposing the cells to 50 Hz electromagnetic fields caused a global activation of the 20S proteasome catalytic components, particularly evident at 72 hours exposure and in the presence of TPA. The finding that EGCG, a natural antioxidant compound, counteracted the field-related pro-oxidant effects demonstrates that the increased proteasome activity was due to an enhancement in intracellular free radicals.

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Related in: MedlinePlus

Effect of ELF-EMFs exposure and TPA treatment on 20S proteasome levels.  Representative autoradiography of 20S proteasome in Caco cells exposed to  ELF-EMFs. The densitometric analysis from six separate blots provided for quantitative analysis of the amount of 20S “core” is presented (a) and a representative Western blot is shown in (b). Equal protein loading was verified by using an anti-GAPDH antibody (c). The immunostaining was performed using an anti-20S proteasome antibody, and the detection was executed by the Enhanced Chemiluminescence Western blot analysis system.
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fig7: Effect of ELF-EMFs exposure and TPA treatment on 20S proteasome levels. Representative autoradiography of 20S proteasome in Caco cells exposed to ELF-EMFs. The densitometric analysis from six separate blots provided for quantitative analysis of the amount of 20S “core” is presented (a) and a representative Western blot is shown in (b). Equal protein loading was verified by using an anti-GAPDH antibody (c). The immunostaining was performed using an anti-20S proteasome antibody, and the detection was executed by the Enhanced Chemiluminescence Western blot analysis system.

Mentions: In order to evaluate whether the differences observed in proteasome functionality were due to changes in 20S proteasome expression levels, we performed immunoblottings using an anti-20S proteasome antibody. As shown in Figure 7, no differences were detected in the proteasome content in lysates obtained from treated and control cells, suggesting that the fields do not affect proteasome expression.


50 Hz extremely low frequency electromagnetic fields enhance protein carbonyl groups content in cancer cells: effects on proteasomal systems.

Eleuteri AM, Amici M, Bonfili L, Cecarini V, Cuccioloni M, Grimaldi S, Giuliani L, Angeletti M, Fioretti E - J. Biomed. Biotechnol. (2009)

Effect of ELF-EMFs exposure and TPA treatment on 20S proteasome levels.  Representative autoradiography of 20S proteasome in Caco cells exposed to  ELF-EMFs. The densitometric analysis from six separate blots provided for quantitative analysis of the amount of 20S “core” is presented (a) and a representative Western blot is shown in (b). Equal protein loading was verified by using an anti-GAPDH antibody (c). The immunostaining was performed using an anti-20S proteasome antibody, and the detection was executed by the Enhanced Chemiluminescence Western blot analysis system.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2722031&req=5

fig7: Effect of ELF-EMFs exposure and TPA treatment on 20S proteasome levels. Representative autoradiography of 20S proteasome in Caco cells exposed to ELF-EMFs. The densitometric analysis from six separate blots provided for quantitative analysis of the amount of 20S “core” is presented (a) and a representative Western blot is shown in (b). Equal protein loading was verified by using an anti-GAPDH antibody (c). The immunostaining was performed using an anti-20S proteasome antibody, and the detection was executed by the Enhanced Chemiluminescence Western blot analysis system.
Mentions: In order to evaluate whether the differences observed in proteasome functionality were due to changes in 20S proteasome expression levels, we performed immunoblottings using an anti-20S proteasome antibody. As shown in Figure 7, no differences were detected in the proteasome content in lysates obtained from treated and control cells, suggesting that the fields do not affect proteasome expression.

Bottom Line: This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins.Caco 2 cells were exposed, for 24-72 hours, to 1 mT, 50 Hz electromagnetic fields.The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology M.C.A., University of Camerino, 62032 Camerino (MC), Italy. annamaria.eleuteri@unicam.it

ABSTRACT
Electromagnetic fields are an assessed cause of prolonging free radicals lifespan. This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins. Caco 2 cells were exposed, for 24-72 hours, to 1 mT, 50 Hz electromagnetic fields. The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected. Exposing the cells to 50 Hz electromagnetic fields caused a global activation of the 20S proteasome catalytic components, particularly evident at 72 hours exposure and in the presence of TPA. The finding that EGCG, a natural antioxidant compound, counteracted the field-related pro-oxidant effects demonstrates that the increased proteasome activity was due to an enhancement in intracellular free radicals.

Show MeSH
Related in: MedlinePlus