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50 Hz extremely low frequency electromagnetic fields enhance protein carbonyl groups content in cancer cells: effects on proteasomal systems.

Eleuteri AM, Amici M, Bonfili L, Cecarini V, Cuccioloni M, Grimaldi S, Giuliani L, Angeletti M, Fioretti E - J. Biomed. Biotechnol. (2009)

Bottom Line: This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins.Caco 2 cells were exposed, for 24-72 hours, to 1 mT, 50 Hz electromagnetic fields.The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology M.C.A., University of Camerino, 62032 Camerino (MC), Italy. annamaria.eleuteri@unicam.it

ABSTRACT
Electromagnetic fields are an assessed cause of prolonging free radicals lifespan. This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins. Caco 2 cells were exposed, for 24-72 hours, to 1 mT, 50 Hz electromagnetic fields. The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected. Exposing the cells to 50 Hz electromagnetic fields caused a global activation of the 20S proteasome catalytic components, particularly evident at 72 hours exposure and in the presence of TPA. The finding that EGCG, a natural antioxidant compound, counteracted the field-related pro-oxidant effects demonstrates that the increased proteasome activity was due to an enhancement in intracellular free radicals.

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Related in: MedlinePlus

Temperature monitoring of exposed samples over time. Direct monitoring of treated sample temperatures before, during and after exposure was performed using a digital multimeter (METEX Mod. M-3630D) equipped with ScopeView software (vers. 1.06) for data logging (the acquisition was carried out at 0.0028 Hz). The temperature probe was placed within the center of the solenoid in a culture dish containing medium with no cells.
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Related In: Results  -  Collection


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fig1: Temperature monitoring of exposed samples over time. Direct monitoring of treated sample temperatures before, during and after exposure was performed using a digital multimeter (METEX Mod. M-3630D) equipped with ScopeView software (vers. 1.06) for data logging (the acquisition was carried out at 0.0028 Hz). The temperature probe was placed within the center of the solenoid in a culture dish containing medium with no cells.

Mentions: Direct monitoring of sample temperatures before, during, and after exposure for both the control and exposed conditions was performed in continuous using a digital multimeter (METEX Mod. M-3630D) controlled by ScopeView software (vers. 1.06) for data logging (the acquisition was carried out at 0.0028 Hz). The temperature probe was placed within the centre of the solenoid (either energized or not) in a culture dish containing medium with no cells: no significant temperature difference between culture media of exposed or unexposed dishes was revealed. Samples temperature was 37.0 ± 0.3°C; see Figure 1.


50 Hz extremely low frequency electromagnetic fields enhance protein carbonyl groups content in cancer cells: effects on proteasomal systems.

Eleuteri AM, Amici M, Bonfili L, Cecarini V, Cuccioloni M, Grimaldi S, Giuliani L, Angeletti M, Fioretti E - J. Biomed. Biotechnol. (2009)

Temperature monitoring of exposed samples over time. Direct monitoring of treated sample temperatures before, during and after exposure was performed using a digital multimeter (METEX Mod. M-3630D) equipped with ScopeView software (vers. 1.06) for data logging (the acquisition was carried out at 0.0028 Hz). The temperature probe was placed within the center of the solenoid in a culture dish containing medium with no cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2722031&req=5

fig1: Temperature monitoring of exposed samples over time. Direct monitoring of treated sample temperatures before, during and after exposure was performed using a digital multimeter (METEX Mod. M-3630D) equipped with ScopeView software (vers. 1.06) for data logging (the acquisition was carried out at 0.0028 Hz). The temperature probe was placed within the center of the solenoid in a culture dish containing medium with no cells.
Mentions: Direct monitoring of sample temperatures before, during, and after exposure for both the control and exposed conditions was performed in continuous using a digital multimeter (METEX Mod. M-3630D) controlled by ScopeView software (vers. 1.06) for data logging (the acquisition was carried out at 0.0028 Hz). The temperature probe was placed within the centre of the solenoid (either energized or not) in a culture dish containing medium with no cells: no significant temperature difference between culture media of exposed or unexposed dishes was revealed. Samples temperature was 37.0 ± 0.3°C; see Figure 1.

Bottom Line: This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins.Caco 2 cells were exposed, for 24-72 hours, to 1 mT, 50 Hz electromagnetic fields.The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology M.C.A., University of Camerino, 62032 Camerino (MC), Italy. annamaria.eleuteri@unicam.it

ABSTRACT
Electromagnetic fields are an assessed cause of prolonging free radicals lifespan. This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins. Caco 2 cells were exposed, for 24-72 hours, to 1 mT, 50 Hz electromagnetic fields. The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected. Exposing the cells to 50 Hz electromagnetic fields caused a global activation of the 20S proteasome catalytic components, particularly evident at 72 hours exposure and in the presence of TPA. The finding that EGCG, a natural antioxidant compound, counteracted the field-related pro-oxidant effects demonstrates that the increased proteasome activity was due to an enhancement in intracellular free radicals.

Show MeSH
Related in: MedlinePlus