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A case of imported Plasmodium ovale malaria.

Han TH, Kim BN, Seong HK - J. Korean Med. Sci. (2006)

Bottom Line: The amplified DNA was sequenced and compared with other registered P. ovale isolates.The isolate in this study was a member of the classic type group.He was treated with hydroxychloroquine and primaquine and discharged following improvement.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Inje University Sanggye Paik Hospital, Korea. kscosby@sanggyepaik.ac.kr

ABSTRACT
There have been reports in Korea of imported malaria cases of four Plasmodium species, but there has been no report of imported Plasmodium ovale malaria confirmed by molecular biological methods. We report an imported case of that was confirmed by Wright-Giemsa-stained peripheral blood smear and nested polymerase chain reaction targeting the small subunit ribosomal RNA gene. The amplified DNA was sequenced and compared with other registered P. ovale isolates. The isolate in this study was a member of the classic type group. The patient was a 44-yr-old male who had worked as a woodcutter in Côte d'Ivoire in tropical West Africa. He was treated with hydroxychloroquine and primaquine and discharged following improvement. In conclusion, P. ovale should be considered as an etiology in the imported malaria in Korea, because the number of travelers to P. ovale endemic regions has recently increased.

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Related in: MedlinePlus

Phylogenetic tree based on the small subunit ribosomal RNA genes of Plasmodium species including four registered P. ovale isolates (Nigerian I/CDC, Papua New Guinea, MAL/MAI, and CAG/Cameroon.) and P. ovale isolate (SM04-119, DQ104413) of this study. SM04-119 is more similar to the isolates of Nigerian I/CDC and Papua New Guinea than those of MAL/MAI and CAG/Cameroon.
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Figure 3: Phylogenetic tree based on the small subunit ribosomal RNA genes of Plasmodium species including four registered P. ovale isolates (Nigerian I/CDC, Papua New Guinea, MAL/MAI, and CAG/Cameroon.) and P. ovale isolate (SM04-119, DQ104413) of this study. SM04-119 is more similar to the isolates of Nigerian I/CDC and Papua New Guinea than those of MAL/MAI and CAG/Cameroon.

Mentions: The PCR product was sequenced at Solgent (Daejeon, Korea). For the SSU rRNA gene, rPLU5 and RPLU6 were used. It was sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, U.S.A.). Sequencing products were resolved with an ABI 3730 XL autoanalyzer (Applied Biosystems). To compare gene sequences for the SSU rRNA genes, Nigerian I/CDC strain (L48997), NAG/Cameroon (AJ00157), MAL/MAI (X99790), and Papua New Guinea (AF145337) were used. We chose P. falciparum (AF145334), P. vivax (AF145335), and P. malariae (AF145336) as an outgroup. Nucleotide sequences were aligned using BioEdit (North Carolina State University, Raleigh, NC, U.S.A.). The aligned sequences were analyzed by the maximum likelihood method using fastDNAmL and distance methods using the neighboring distance method included in PHYLIP (Version 3.63, University of Washington, Seattle, WA, U.S.A.). The alignment of the P. ovale partial sequence of the SSU rRNA gene showed that our isolate (SM04-119, DQ104413) was more similar to isolates of Nigerian I/CDC and Papua New Guinea (identity 98.3% and 97.9%, respectively) than to those of NGA/Cameroon and MAL/MAI (95.8% and 95.4% identity, respectively) (Fig. 3).


A case of imported Plasmodium ovale malaria.

Han TH, Kim BN, Seong HK - J. Korean Med. Sci. (2006)

Phylogenetic tree based on the small subunit ribosomal RNA genes of Plasmodium species including four registered P. ovale isolates (Nigerian I/CDC, Papua New Guinea, MAL/MAI, and CAG/Cameroon.) and P. ovale isolate (SM04-119, DQ104413) of this study. SM04-119 is more similar to the isolates of Nigerian I/CDC and Papua New Guinea than those of MAL/MAI and CAG/Cameroon.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2722008&req=5

Figure 3: Phylogenetic tree based on the small subunit ribosomal RNA genes of Plasmodium species including four registered P. ovale isolates (Nigerian I/CDC, Papua New Guinea, MAL/MAI, and CAG/Cameroon.) and P. ovale isolate (SM04-119, DQ104413) of this study. SM04-119 is more similar to the isolates of Nigerian I/CDC and Papua New Guinea than those of MAL/MAI and CAG/Cameroon.
Mentions: The PCR product was sequenced at Solgent (Daejeon, Korea). For the SSU rRNA gene, rPLU5 and RPLU6 were used. It was sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, U.S.A.). Sequencing products were resolved with an ABI 3730 XL autoanalyzer (Applied Biosystems). To compare gene sequences for the SSU rRNA genes, Nigerian I/CDC strain (L48997), NAG/Cameroon (AJ00157), MAL/MAI (X99790), and Papua New Guinea (AF145337) were used. We chose P. falciparum (AF145334), P. vivax (AF145335), and P. malariae (AF145336) as an outgroup. Nucleotide sequences were aligned using BioEdit (North Carolina State University, Raleigh, NC, U.S.A.). The aligned sequences were analyzed by the maximum likelihood method using fastDNAmL and distance methods using the neighboring distance method included in PHYLIP (Version 3.63, University of Washington, Seattle, WA, U.S.A.). The alignment of the P. ovale partial sequence of the SSU rRNA gene showed that our isolate (SM04-119, DQ104413) was more similar to isolates of Nigerian I/CDC and Papua New Guinea (identity 98.3% and 97.9%, respectively) than to those of NGA/Cameroon and MAL/MAI (95.8% and 95.4% identity, respectively) (Fig. 3).

Bottom Line: The amplified DNA was sequenced and compared with other registered P. ovale isolates.The isolate in this study was a member of the classic type group.He was treated with hydroxychloroquine and primaquine and discharged following improvement.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Inje University Sanggye Paik Hospital, Korea. kscosby@sanggyepaik.ac.kr

ABSTRACT
There have been reports in Korea of imported malaria cases of four Plasmodium species, but there has been no report of imported Plasmodium ovale malaria confirmed by molecular biological methods. We report an imported case of that was confirmed by Wright-Giemsa-stained peripheral blood smear and nested polymerase chain reaction targeting the small subunit ribosomal RNA gene. The amplified DNA was sequenced and compared with other registered P. ovale isolates. The isolate in this study was a member of the classic type group. The patient was a 44-yr-old male who had worked as a woodcutter in Côte d'Ivoire in tropical West Africa. He was treated with hydroxychloroquine and primaquine and discharged following improvement. In conclusion, P. ovale should be considered as an etiology in the imported malaria in Korea, because the number of travelers to P. ovale endemic regions has recently increased.

Show MeSH
Related in: MedlinePlus