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Expression of cyclooxygenase-2, p53 and Ki-67 in gastric cancer.

Joo YE, Chung IJ, Park YK, Koh YS, Lee JH, Park CH, Lee WS, Kim HS, Choi SK, Rew JS, Park CS, Kim SJ - J. Korean Med. Sci. (2006)

Bottom Line: The Ki-67 labeling index (LI) was counted by Ki-67 immunostaining.However, there was no association between COX-2 or p53 expression and survival. p53 expression did not correlate with COX-2 expression.There was no significant difference in various clinicopathological variables between Ki-67 LI subgroups.

View Article: PubMed Central - PubMed

Affiliation: Gastrointestinal Cancer Research Program, Department of Internal Medicine, Chonnam National University School, Gwangju, Korea. yejoo@jnu.ac.kr

ABSTRACT
It has been reported that p53 mutation may contribute to upregulate cyclooxygenase (COX)-2 expression that is observed in malignant tissues. These molecules are involved in carcinogenesis by affecting tumor cell proliferation. The aim of this study was to examine the relationship between COX-2 or p53 expression and clinico-pathological characteristics including tumor cell proliferation in gastric cancer. COX-2 and p53 expressions were investigated with immunostaining, in tissue specimens obtained from 119 patients who underwent surgery for gastric cancer. The Ki-67 labeling index (LI) was counted by Ki-67 immunostaining. COX-2 and p53 expressions correlated significantly with depth of tumor invasion. However, there was no association between COX-2 or p53 expression and survival. p53 expression did not correlate with COX-2 expression. There was no significant difference in various clinicopathological variables between Ki-67 LI subgroups. The mean Ki-67 LI value of COX-2 positive tumors was significantly higher than that of negative tumors. The mean Ki-67 LI value of p53 positive tumors was not significantly higher than that of negative tumors. The mean Ki-67 LI value of both COX-2 and p53 positive tumors was significantly higher than that of both negative tumors. These results imply that COX-2 expression is associated with tumor cell proliferation of gastric cancer.

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Immunoreactivity of COX-2 in gastric cancer tissues. COX-2 immunoreactivity is predominantly detected in the cytoplasm of cancer cells (×200).
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Figure 1: Immunoreactivity of COX-2 in gastric cancer tissues. COX-2 immunoreactivity is predominantly detected in the cytoplasm of cancer cells (×200).

Mentions: COX-2 immunoreactivity was cytoplasmic and was almost exclusively restricted to cancerous areas of pathologic specimens (Fig. 1). In some tumors, the COX-2 staining was diffuse, and in other tumors it was localized. In the normal gastric epithelia of non-cancerous area, no COX-2 immunoreactivity was observed. Immunoreactivity specific for p53 protein was evident in the nuclei of cancer cells and heterogenously distributed. Ki-67 immunoreactivity was almost found in the nuclei of cancer cells. Positive cells were frequent in the advancing margin of the tumor. Based on our criteria, expression of COX-2 and p53 in gastric cancer tissues, was demonstrated in 60.5% (72/119) and 34.5% (41/119), respectively.


Expression of cyclooxygenase-2, p53 and Ki-67 in gastric cancer.

Joo YE, Chung IJ, Park YK, Koh YS, Lee JH, Park CH, Lee WS, Kim HS, Choi SK, Rew JS, Park CS, Kim SJ - J. Korean Med. Sci. (2006)

Immunoreactivity of COX-2 in gastric cancer tissues. COX-2 immunoreactivity is predominantly detected in the cytoplasm of cancer cells (×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2721998&req=5

Figure 1: Immunoreactivity of COX-2 in gastric cancer tissues. COX-2 immunoreactivity is predominantly detected in the cytoplasm of cancer cells (×200).
Mentions: COX-2 immunoreactivity was cytoplasmic and was almost exclusively restricted to cancerous areas of pathologic specimens (Fig. 1). In some tumors, the COX-2 staining was diffuse, and in other tumors it was localized. In the normal gastric epithelia of non-cancerous area, no COX-2 immunoreactivity was observed. Immunoreactivity specific for p53 protein was evident in the nuclei of cancer cells and heterogenously distributed. Ki-67 immunoreactivity was almost found in the nuclei of cancer cells. Positive cells were frequent in the advancing margin of the tumor. Based on our criteria, expression of COX-2 and p53 in gastric cancer tissues, was demonstrated in 60.5% (72/119) and 34.5% (41/119), respectively.

Bottom Line: The Ki-67 labeling index (LI) was counted by Ki-67 immunostaining.However, there was no association between COX-2 or p53 expression and survival. p53 expression did not correlate with COX-2 expression.There was no significant difference in various clinicopathological variables between Ki-67 LI subgroups.

View Article: PubMed Central - PubMed

Affiliation: Gastrointestinal Cancer Research Program, Department of Internal Medicine, Chonnam National University School, Gwangju, Korea. yejoo@jnu.ac.kr

ABSTRACT
It has been reported that p53 mutation may contribute to upregulate cyclooxygenase (COX)-2 expression that is observed in malignant tissues. These molecules are involved in carcinogenesis by affecting tumor cell proliferation. The aim of this study was to examine the relationship between COX-2 or p53 expression and clinico-pathological characteristics including tumor cell proliferation in gastric cancer. COX-2 and p53 expressions were investigated with immunostaining, in tissue specimens obtained from 119 patients who underwent surgery for gastric cancer. The Ki-67 labeling index (LI) was counted by Ki-67 immunostaining. COX-2 and p53 expressions correlated significantly with depth of tumor invasion. However, there was no association between COX-2 or p53 expression and survival. p53 expression did not correlate with COX-2 expression. There was no significant difference in various clinicopathological variables between Ki-67 LI subgroups. The mean Ki-67 LI value of COX-2 positive tumors was significantly higher than that of negative tumors. The mean Ki-67 LI value of p53 positive tumors was not significantly higher than that of negative tumors. The mean Ki-67 LI value of both COX-2 and p53 positive tumors was significantly higher than that of both negative tumors. These results imply that COX-2 expression is associated with tumor cell proliferation of gastric cancer.

Show MeSH
Related in: MedlinePlus