Limits...
mRNA expression and RNA editing (2451 C-to-U) of IL-12 receptor beta2 in adult atopic patients.

Kim EJ, Lee WM, Ha JS, Ryoo NH, Jeon DS, Kim JR - J. Korean Med. Sci. (2006)

Bottom Line: IL-12 acts through interaction with its receptor, especially beta(2) subunit.Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy.In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count.

View Article: PubMed Central - PubMed

Affiliation: Daegu Kyoungbook Blood Center, Korea Red Cross, Daegu, Korea.

ABSTRACT
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta(2) subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta(2) mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta(2) mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy.

Show MeSH

Related in: MedlinePlus

Results of sequencing analysis of IL-12Rβ2 cDNA (A) and genomic DNA (B). Both bases of nucleotide 2451 of cDNA and genomic DNA are cytosines in atopic patients and healthy controls. Arrows indicate the base composition of nucleotide 2451 on exon 13.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2721931&req=5

Figure 3: Results of sequencing analysis of IL-12Rβ2 cDNA (A) and genomic DNA (B). Both bases of nucleotide 2451 of cDNA and genomic DNA are cytosines in atopic patients and healthy controls. Arrows indicate the base composition of nucleotide 2451 on exon 13.

Mentions: In the results of sequencing analysis, all the nucleotide 2451 was cytidine (C) in cDNA as well as genomic DNA from atopic patients and healthy controls (Fig. 3). Therefore, the RNA editing phenomenon, C-to-U conversion at nucleotide 2451 of IL-12Rβ2 was not found in present study.


mRNA expression and RNA editing (2451 C-to-U) of IL-12 receptor beta2 in adult atopic patients.

Kim EJ, Lee WM, Ha JS, Ryoo NH, Jeon DS, Kim JR - J. Korean Med. Sci. (2006)

Results of sequencing analysis of IL-12Rβ2 cDNA (A) and genomic DNA (B). Both bases of nucleotide 2451 of cDNA and genomic DNA are cytosines in atopic patients and healthy controls. Arrows indicate the base composition of nucleotide 2451 on exon 13.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2721931&req=5

Figure 3: Results of sequencing analysis of IL-12Rβ2 cDNA (A) and genomic DNA (B). Both bases of nucleotide 2451 of cDNA and genomic DNA are cytosines in atopic patients and healthy controls. Arrows indicate the base composition of nucleotide 2451 on exon 13.
Mentions: In the results of sequencing analysis, all the nucleotide 2451 was cytidine (C) in cDNA as well as genomic DNA from atopic patients and healthy controls (Fig. 3). Therefore, the RNA editing phenomenon, C-to-U conversion at nucleotide 2451 of IL-12Rβ2 was not found in present study.

Bottom Line: IL-12 acts through interaction with its receptor, especially beta(2) subunit.Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy.In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count.

View Article: PubMed Central - PubMed

Affiliation: Daegu Kyoungbook Blood Center, Korea Red Cross, Daegu, Korea.

ABSTRACT
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta(2) subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta(2) mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta(2) mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy.

Show MeSH
Related in: MedlinePlus