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mRNA expression and RNA editing (2451 C-to-U) of IL-12 receptor beta2 in adult atopic patients.

Kim EJ, Lee WM, Ha JS, Ryoo NH, Jeon DS, Kim JR - J. Korean Med. Sci. (2006)

Bottom Line: IL-12 acts through interaction with its receptor, especially beta(2) subunit.Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy.In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count.

View Article: PubMed Central - PubMed

Affiliation: Daegu Kyoungbook Blood Center, Korea Red Cross, Daegu, Korea.

ABSTRACT
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta(2) subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta(2) mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta(2) mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy.

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Comparison of IL-12Rβ2/GAPDH ratio between healthy controls (n=54) and atopic patients (n=80). The mRNA expression of IL-12Rβ2 is significantly lower in atopic patients than healthy controls (p<0.05).
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Figure 2: Comparison of IL-12Rβ2/GAPDH ratio between healthy controls (n=54) and atopic patients (n=80). The mRNA expression of IL-12Rβ2 is significantly lower in atopic patients than healthy controls (p<0.05).

Mentions: The PHA stimulated PBMCs from atopic patients had significantly lower expression of IL-12Rβ2 mRNA than the cells from healthy controls (p<0.05) (Fig. 2). The average IL-12Rβ2/GAPDH ratio was 0.035±0.02 in healthy controls and 0.018±0.047 in atopic patients. When patients were divided into 3 disease groups (asthma, atopic dermatitis, allergic rhinitis), each disease group showed lower IL-12Rβ2 mRNA expression than controls (p<0.05, respectively), also. But there was no significant difference of IL-12Rβ2 mRNA expression between 3 groups (p>0.05) (Table 2).


mRNA expression and RNA editing (2451 C-to-U) of IL-12 receptor beta2 in adult atopic patients.

Kim EJ, Lee WM, Ha JS, Ryoo NH, Jeon DS, Kim JR - J. Korean Med. Sci. (2006)

Comparison of IL-12Rβ2/GAPDH ratio between healthy controls (n=54) and atopic patients (n=80). The mRNA expression of IL-12Rβ2 is significantly lower in atopic patients than healthy controls (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2721931&req=5

Figure 2: Comparison of IL-12Rβ2/GAPDH ratio between healthy controls (n=54) and atopic patients (n=80). The mRNA expression of IL-12Rβ2 is significantly lower in atopic patients than healthy controls (p<0.05).
Mentions: The PHA stimulated PBMCs from atopic patients had significantly lower expression of IL-12Rβ2 mRNA than the cells from healthy controls (p<0.05) (Fig. 2). The average IL-12Rβ2/GAPDH ratio was 0.035±0.02 in healthy controls and 0.018±0.047 in atopic patients. When patients were divided into 3 disease groups (asthma, atopic dermatitis, allergic rhinitis), each disease group showed lower IL-12Rβ2 mRNA expression than controls (p<0.05, respectively), also. But there was no significant difference of IL-12Rβ2 mRNA expression between 3 groups (p>0.05) (Table 2).

Bottom Line: IL-12 acts through interaction with its receptor, especially beta(2) subunit.Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy.In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count.

View Article: PubMed Central - PubMed

Affiliation: Daegu Kyoungbook Blood Center, Korea Red Cross, Daegu, Korea.

ABSTRACT
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-gamma which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially beta(2) subunit. In several studies, the low production of IFN-gamma in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor beta(2) (IL-12R beta(2)) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12R beta(2) mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12R beta(2) mRNA and serum total IgE or blood eosinophil count. Reduced IL-12R beta(2) mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-gamma production and this may contribute to Th2-skewed immune response in atopy.

Show MeSH
Related in: MedlinePlus