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Cyclooxygenase-2 and inducible nitric oxide synthase expression in thyroid neoplasms and their clinicopathological correlation.

Kim KH, Kim SH, Kim SH, Back JH, Park MJ, Kim JM - J. Korean Med. Sci. (2006)

Bottom Line: The results showed that COX-2 and iNOS were frequently expressed in the PTC and HN specimens, and iNOS was more frequently overexpressed in the FC specimens than in the FA specimens.In PTC, COX-2 and iNOS were significantly overexpressed in patients over 45 yr of age (p=0.029, p=0.041), and iNOS expression was increased in patients with a large primary tumor (p=0.028).These results suggest that the upregulation of COX-2 and iNOS may contribute to the tumor progression of thyroid gland, particularly in PTC and HN, and iNOS may play an adjuvant role during the tumor progression of FC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Molecular Medicine, Eulji University School of Medicine, Daejeon, Korea.

ABSTRACT
To evaluate the expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in thyroid neoplasms in a Korean population, we studied a total of 154 cases: papillary carcinoma of classical type (PTC), 86; follicular adenoma (FA), 21; follicular carcinoma (FC), 35; medullary carcinoma (MC), 3; undifferentiated carcinoma (UC), 5; and Hurthle cell neoplasm (HN), 4. Using immunohistochemical staining, COX-2 expression was detected in 62 (72.1%) PTC specimens, 5 (23.8%) FA specimens, 10 (28.6%) FC specimens, 0 (0.0%) MC specimens, 1 (20.0%) UC specimen, and 3 (75%) HN specimens. iNOS expression was detected in 66 (76.7%) PTC specimens, 4 (19.0%) FA specimens, 13 (37.1%) FC specimens, 0 (0.0%) MC specimens, 3 (60.0%) UC specimens, and 4 (100%) HN specimens. The results showed that COX-2 and iNOS were frequently expressed in the PTC and HN specimens, and iNOS was more frequently overexpressed in the FC specimens than in the FA specimens. In PTC, COX-2 and iNOS were significantly overexpressed in patients over 45 yr of age (p=0.029, p=0.041), and iNOS expression was increased in patients with a large primary tumor (p=0.028). These results suggest that the upregulation of COX-2 and iNOS may contribute to the tumor progression of thyroid gland, particularly in PTC and HN, and iNOS may play an adjuvant role during the tumor progression of FC.

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Immunohistochemical staining demonstrates the intensity of iNOS expression in the cytoplasm of papillary carcinoma cases. (A) score 0; (B) score 1; (C) score 2; (D) score 3 (×400).
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Figure 2: Immunohistochemical staining demonstrates the intensity of iNOS expression in the cytoplasm of papillary carcinoma cases. (A) score 0; (B) score 1; (C) score 2; (D) score 3 (×400).

Mentions: The intensity of the staining was scored 0-3 (absent, mild, moderate, strong) (Fig. 1, 2) and the area of positivity was estimated as the percentage of the total area of the tumor (<10%, 10-50%, and >50%). The final score was a combination of these two variables and was calculated as follows: score 0, negative staining or intensity 1+area <10%; score 1, intensity 1+area between 10% and 100% or intensity 2-3+area <10%; score 2, intensity 2+area >50% or intensity 3+area between 10% and 50%; score 3, intensity 3+area >50%. A final score of 1-3 was defined as positive immunoreactivity. The scoring was performed independently and in a blinded manner by three pathologists who had no knowledge of the clinical data. Discrepant scoring was determined by consensus.


Cyclooxygenase-2 and inducible nitric oxide synthase expression in thyroid neoplasms and their clinicopathological correlation.

Kim KH, Kim SH, Kim SH, Back JH, Park MJ, Kim JM - J. Korean Med. Sci. (2006)

Immunohistochemical staining demonstrates the intensity of iNOS expression in the cytoplasm of papillary carcinoma cases. (A) score 0; (B) score 1; (C) score 2; (D) score 3 (×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2721930&req=5

Figure 2: Immunohistochemical staining demonstrates the intensity of iNOS expression in the cytoplasm of papillary carcinoma cases. (A) score 0; (B) score 1; (C) score 2; (D) score 3 (×400).
Mentions: The intensity of the staining was scored 0-3 (absent, mild, moderate, strong) (Fig. 1, 2) and the area of positivity was estimated as the percentage of the total area of the tumor (<10%, 10-50%, and >50%). The final score was a combination of these two variables and was calculated as follows: score 0, negative staining or intensity 1+area <10%; score 1, intensity 1+area between 10% and 100% or intensity 2-3+area <10%; score 2, intensity 2+area >50% or intensity 3+area between 10% and 50%; score 3, intensity 3+area >50%. A final score of 1-3 was defined as positive immunoreactivity. The scoring was performed independently and in a blinded manner by three pathologists who had no knowledge of the clinical data. Discrepant scoring was determined by consensus.

Bottom Line: The results showed that COX-2 and iNOS were frequently expressed in the PTC and HN specimens, and iNOS was more frequently overexpressed in the FC specimens than in the FA specimens.In PTC, COX-2 and iNOS were significantly overexpressed in patients over 45 yr of age (p=0.029, p=0.041), and iNOS expression was increased in patients with a large primary tumor (p=0.028).These results suggest that the upregulation of COX-2 and iNOS may contribute to the tumor progression of thyroid gland, particularly in PTC and HN, and iNOS may play an adjuvant role during the tumor progression of FC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Molecular Medicine, Eulji University School of Medicine, Daejeon, Korea.

ABSTRACT
To evaluate the expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in thyroid neoplasms in a Korean population, we studied a total of 154 cases: papillary carcinoma of classical type (PTC), 86; follicular adenoma (FA), 21; follicular carcinoma (FC), 35; medullary carcinoma (MC), 3; undifferentiated carcinoma (UC), 5; and Hurthle cell neoplasm (HN), 4. Using immunohistochemical staining, COX-2 expression was detected in 62 (72.1%) PTC specimens, 5 (23.8%) FA specimens, 10 (28.6%) FC specimens, 0 (0.0%) MC specimens, 1 (20.0%) UC specimen, and 3 (75%) HN specimens. iNOS expression was detected in 66 (76.7%) PTC specimens, 4 (19.0%) FA specimens, 13 (37.1%) FC specimens, 0 (0.0%) MC specimens, 3 (60.0%) UC specimens, and 4 (100%) HN specimens. The results showed that COX-2 and iNOS were frequently expressed in the PTC and HN specimens, and iNOS was more frequently overexpressed in the FC specimens than in the FA specimens. In PTC, COX-2 and iNOS were significantly overexpressed in patients over 45 yr of age (p=0.029, p=0.041), and iNOS expression was increased in patients with a large primary tumor (p=0.028). These results suggest that the upregulation of COX-2 and iNOS may contribute to the tumor progression of thyroid gland, particularly in PTC and HN, and iNOS may play an adjuvant role during the tumor progression of FC.

Show MeSH
Related in: MedlinePlus