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Quantitative analysis of epithelial cells in urine from men with and without urethritis: implications for studying epithelial: pathogen interactions in vivo.

Wiggins R, Horner PJ, Whittington K, Holmes CH - BMC Res Notes (2009)

Bottom Line: Squamous, but not transitional, cell numbers correlated with leukocyte numbers (Spearman's rho = 0.216 p = 0.045 and rho = 0.171 and p = 0.113, respectively).Transitional cells were morphologically heterogeneous and appeared to display complex cytokeratin phenotypes.These would provide novel opportunities for studying cellular interactions of C. trachomatis in male urethral infections, about which little is currently known.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Sciences South Bristol, Obstetrics and Gynaecology, University of Bristol, St Michael's Hospital, Southwell St,Bristol, UK. rcw502@york.ac.uk

ABSTRACT

Background: Epithelial cells in first catch urine (FCU) specimens from 87 men with and without urethritis were quantified. Epithelial cells were broadly categorised into transitional and squamous populations using morphological characteristics and immunostaining with anti-pan leukocyte and anti-cytokeratin monoclonal antibodies.

Findings: The majority (77/87 = 89%) of samples contained both transitional (76/87 = 87%; range 1 x 10(4) - 6 x 10(5), median 6 x 10(4)) and squamous (57/87 = 66%; range 1 x 10(4) - 8 x 10(5), median 2 x 10(4)) epithelial cells. The number of transitional cells correlated with the number of squamous cells (Spearman's rho = 0.697 p < 0.001). Squamous, but not transitional, cell numbers correlated with leukocyte numbers (Spearman's rho = 0.216 p = 0.045 and rho = 0.171 and p = 0.113, respectively). However there was no significant difference in epithelial cell numbers between men with and without urethritis. Nevertheless, some men with urethritis had relatively high numbers of transitional cells in their FCU. Transitional cells were morphologically heterogeneous and appeared to display complex cytokeratin phenotypes.

Conclusion: Further studies are required to explore the complexity of epithelial cell populations in urine. These would provide novel opportunities for studying cellular interactions of C. trachomatis in male urethral infections, about which little is currently known.

No MeSH data available.


Related in: MedlinePlus

a&b: Epithelial count (ranked) and leukocyte count (by patient sample) in urethritis positive (a) and urethritis negative (b) FCU specimens.
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Figure 2: a&b: Epithelial count (ranked) and leukocyte count (by patient sample) in urethritis positive (a) and urethritis negative (b) FCU specimens.

Mentions: Morphological characteristics and anti-CD45 and anti-cytokeratin reactivities of leukocytes and epithelial cells detected in the FCU specimens are illustrated in Fig. 1. Quantitative analysis of these populations is presented in Fig. 2. Figure 1a shows a FCU specimen in which the overwhelmingly predominant cell type was CD45-positive leukocytes, the majority of these being PMNLs [6]. While the majority of FCU specimens were found to contain at least some epithelial cells (see Fig. 2a &2b below), there was a wide variation in the proportion of CD45-positive leukocytes and CD45-negative epithelial cells. For example, the specimen illustrated in Fig. 1b, as well as containing CD45-positive leukocytes, also contained substantial numbers of CD45-negative epithelial cells. These latter cells exhibited a characteristic transitional morphology, displaying a typically round shape with a centrally disposed, round nucleus and high nuclear to cytoplasmic ratio (Fig. 1b).


Quantitative analysis of epithelial cells in urine from men with and without urethritis: implications for studying epithelial: pathogen interactions in vivo.

Wiggins R, Horner PJ, Whittington K, Holmes CH - BMC Res Notes (2009)

a&b: Epithelial count (ranked) and leukocyte count (by patient sample) in urethritis positive (a) and urethritis negative (b) FCU specimens.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719657&req=5

Figure 2: a&b: Epithelial count (ranked) and leukocyte count (by patient sample) in urethritis positive (a) and urethritis negative (b) FCU specimens.
Mentions: Morphological characteristics and anti-CD45 and anti-cytokeratin reactivities of leukocytes and epithelial cells detected in the FCU specimens are illustrated in Fig. 1. Quantitative analysis of these populations is presented in Fig. 2. Figure 1a shows a FCU specimen in which the overwhelmingly predominant cell type was CD45-positive leukocytes, the majority of these being PMNLs [6]. While the majority of FCU specimens were found to contain at least some epithelial cells (see Fig. 2a &2b below), there was a wide variation in the proportion of CD45-positive leukocytes and CD45-negative epithelial cells. For example, the specimen illustrated in Fig. 1b, as well as containing CD45-positive leukocytes, also contained substantial numbers of CD45-negative epithelial cells. These latter cells exhibited a characteristic transitional morphology, displaying a typically round shape with a centrally disposed, round nucleus and high nuclear to cytoplasmic ratio (Fig. 1b).

Bottom Line: Squamous, but not transitional, cell numbers correlated with leukocyte numbers (Spearman's rho = 0.216 p = 0.045 and rho = 0.171 and p = 0.113, respectively).Transitional cells were morphologically heterogeneous and appeared to display complex cytokeratin phenotypes.These would provide novel opportunities for studying cellular interactions of C. trachomatis in male urethral infections, about which little is currently known.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Sciences South Bristol, Obstetrics and Gynaecology, University of Bristol, St Michael's Hospital, Southwell St,Bristol, UK. rcw502@york.ac.uk

ABSTRACT

Background: Epithelial cells in first catch urine (FCU) specimens from 87 men with and without urethritis were quantified. Epithelial cells were broadly categorised into transitional and squamous populations using morphological characteristics and immunostaining with anti-pan leukocyte and anti-cytokeratin monoclonal antibodies.

Findings: The majority (77/87 = 89%) of samples contained both transitional (76/87 = 87%; range 1 x 10(4) - 6 x 10(5), median 6 x 10(4)) and squamous (57/87 = 66%; range 1 x 10(4) - 8 x 10(5), median 2 x 10(4)) epithelial cells. The number of transitional cells correlated with the number of squamous cells (Spearman's rho = 0.697 p < 0.001). Squamous, but not transitional, cell numbers correlated with leukocyte numbers (Spearman's rho = 0.216 p = 0.045 and rho = 0.171 and p = 0.113, respectively). However there was no significant difference in epithelial cell numbers between men with and without urethritis. Nevertheless, some men with urethritis had relatively high numbers of transitional cells in their FCU. Transitional cells were morphologically heterogeneous and appeared to display complex cytokeratin phenotypes.

Conclusion: Further studies are required to explore the complexity of epithelial cell populations in urine. These would provide novel opportunities for studying cellular interactions of C. trachomatis in male urethral infections, about which little is currently known.

No MeSH data available.


Related in: MedlinePlus