Limits...
Does unrestrained single-chamber plethysmography provide a valid assessment of airway responsiveness in allergic BALB/c mice?

Zhang Q, Lai K, Xie J, Chen G, Zhong N - Respir. Res. (2009)

Bottom Line: Compared with controls, a significant increase in airway responsiveness was shown in the OVA-1D-N group (P < 0.05) but not in the OVA-1D-I group.The invasive measurement is specific to lower airway.Penh measurement (done as a screening procedure), must be confirmed by a direct invasive measurement specific to lower airway in evaluating lower airway responsiveness.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Respiratory Disease (Guangzhou Medical University), The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, PR China. Dr.zhang68@yahoo.com

ABSTRACT

Background: Unrestrained plethysmography has been used to monitor bronchoconstriction because of its ease of use and ability to measure airway responsiveness in conscious animals. However, its reliability remains controversial.

Objective: To investigate if unrestrained plethysmography could provide a valid interpretation of airway responsiveness in allergic BALB/c mice.

Methods: Ovalbumin sensitized BALB/c mice were randomized to receive either a single-dose Ovalbumin challenge (OVA-1D group) or a three-dose Ovalbumin challenge (OVA-3D group). The OVA-1D group was further divided into OVA-1D-I (measured invasively, using lung resistance as the index of responsiveness) and OVA-1D-N group (measured non-invasively, using Penh as the index of responsiveness). Similarly the OVA-3D group was divided into OVA-3D-I and OVA-3D-N groups based on the above methods. The control groups were sensitized and challenged with normal saline. Bronchial alveolar lavage fluid was taken and airway histopathology was evaluated for airway inflammation. Nasal responsiveness was tested with histamine challenge.

Results: Compared with controls, a significant increase in airway responsiveness was shown in the OVA-1D-N group (P < 0.05) but not in the OVA-1D-I group. Both OVA-3D-I and OVA-3D-N groups showed higher responsiveness than their controls (P < 0.05). The nasal mucosa was infiltrated by eosinophic cells in all Ovalbumin immunized groups. Sneezing or nasal rubbing in allergic groups appeared more frequent than that in the control groups.

Conclusion: Penh can not be used as a surrogate for airway resistance. The invasive measurement is specific to lower airway. Penh measurement (done as a screening procedure), must be confirmed by a direct invasive measurement specific to lower airway in evaluating lower airway responsiveness.

Show MeSH
Representative hematoxylin and eosin-stained pharyngeal portion mucosa collected after airway responsiveness measured by Penh measurements. (A) Light Microscopic image (50 fold magnification) of Control mice. (B) Magnification of (A), 400 fold magnification. (C) OVA sensitized with single-dose OVA challenge, airway responsiveness was carried out by Penh measurements. (D) OVA sensitized with three-dose OVA challenge, airway responsiveness was carried out by Penh measurements.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2719610&req=5

Figure 6: Representative hematoxylin and eosin-stained pharyngeal portion mucosa collected after airway responsiveness measured by Penh measurements. (A) Light Microscopic image (50 fold magnification) of Control mice. (B) Magnification of (A), 400 fold magnification. (C) OVA sensitized with single-dose OVA challenge, airway responsiveness was carried out by Penh measurements. (D) OVA sensitized with three-dose OVA challenge, airway responsiveness was carried out by Penh measurements.

Mentions: Both in OVA-1D group and OVA-3D group, there was obvious inflammation in upper airway [see figure 6].


Does unrestrained single-chamber plethysmography provide a valid assessment of airway responsiveness in allergic BALB/c mice?

Zhang Q, Lai K, Xie J, Chen G, Zhong N - Respir. Res. (2009)

Representative hematoxylin and eosin-stained pharyngeal portion mucosa collected after airway responsiveness measured by Penh measurements. (A) Light Microscopic image (50 fold magnification) of Control mice. (B) Magnification of (A), 400 fold magnification. (C) OVA sensitized with single-dose OVA challenge, airway responsiveness was carried out by Penh measurements. (D) OVA sensitized with three-dose OVA challenge, airway responsiveness was carried out by Penh measurements.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719610&req=5

Figure 6: Representative hematoxylin and eosin-stained pharyngeal portion mucosa collected after airway responsiveness measured by Penh measurements. (A) Light Microscopic image (50 fold magnification) of Control mice. (B) Magnification of (A), 400 fold magnification. (C) OVA sensitized with single-dose OVA challenge, airway responsiveness was carried out by Penh measurements. (D) OVA sensitized with three-dose OVA challenge, airway responsiveness was carried out by Penh measurements.
Mentions: Both in OVA-1D group and OVA-3D group, there was obvious inflammation in upper airway [see figure 6].

Bottom Line: Compared with controls, a significant increase in airway responsiveness was shown in the OVA-1D-N group (P < 0.05) but not in the OVA-1D-I group.The invasive measurement is specific to lower airway.Penh measurement (done as a screening procedure), must be confirmed by a direct invasive measurement specific to lower airway in evaluating lower airway responsiveness.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Respiratory Disease (Guangzhou Medical University), The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, PR China. Dr.zhang68@yahoo.com

ABSTRACT

Background: Unrestrained plethysmography has been used to monitor bronchoconstriction because of its ease of use and ability to measure airway responsiveness in conscious animals. However, its reliability remains controversial.

Objective: To investigate if unrestrained plethysmography could provide a valid interpretation of airway responsiveness in allergic BALB/c mice.

Methods: Ovalbumin sensitized BALB/c mice were randomized to receive either a single-dose Ovalbumin challenge (OVA-1D group) or a three-dose Ovalbumin challenge (OVA-3D group). The OVA-1D group was further divided into OVA-1D-I (measured invasively, using lung resistance as the index of responsiveness) and OVA-1D-N group (measured non-invasively, using Penh as the index of responsiveness). Similarly the OVA-3D group was divided into OVA-3D-I and OVA-3D-N groups based on the above methods. The control groups were sensitized and challenged with normal saline. Bronchial alveolar lavage fluid was taken and airway histopathology was evaluated for airway inflammation. Nasal responsiveness was tested with histamine challenge.

Results: Compared with controls, a significant increase in airway responsiveness was shown in the OVA-1D-N group (P < 0.05) but not in the OVA-1D-I group. Both OVA-3D-I and OVA-3D-N groups showed higher responsiveness than their controls (P < 0.05). The nasal mucosa was infiltrated by eosinophic cells in all Ovalbumin immunized groups. Sneezing or nasal rubbing in allergic groups appeared more frequent than that in the control groups.

Conclusion: Penh can not be used as a surrogate for airway resistance. The invasive measurement is specific to lower airway. Penh measurement (done as a screening procedure), must be confirmed by a direct invasive measurement specific to lower airway in evaluating lower airway responsiveness.

Show MeSH